ANTIBACTERIAL EVALUATION OF METHANOLIC RHIZOME EXTRACT FROM AN IN VIVO AND IN VITRO GROWN PTERIDOPHYTE, DRYNARIA QUERCIFOLIA (LINN.) J SMITH

  • Ranjan Padhy
  • Santosh Kumar Dash

Abstract

ABSTRACT
Objective: Drynaria quercifolia (Linn.) J. Smith has been used in many herbal folklore medicines in Ganjam - Gajapati districts of Odisha state.
Study pertains to its in vivo and in vitro antibacterial activities were undertaken to scientifically validate one of such therapeutic claim raised in the
literatures, foreseeing the vulnerable state of the species.
Methods: Methanolic extract from both the in vivo grown plant and in vitro grown callus were prepared and tested for their antibacterial efficacy
against a wide range of bacterial pathogens concerned. Both agar well diffusion and disk diffusion methods were followed for evaluation of
antibacterial activities. Development of callus was done using modified MS media supplemented with 20/gl sucrose and varied concentrations of
auxins and cytokinins and the extract was prepared thereof. Antibacterial potency of both the extracts was measured in terms of zone of inhibition
and statistical analysis was performed using Graph pad prism 6 software.
Results: Methanolic extracts from both in vivo and in vitro samples had broad spectrum antibacterial activity on series of bacteria; however, bacterial
species like Salmonella typhi, Shigella flexinneri, Streptococcus pneumoniae were resistant to both the samples. Further, the in vitro sample showed a
higher degree of inhibition compared to the in vivo sample, was revealed.
Conclusion: Study explored that the plant species is a potential source of antibacterial activity suggesting its in vitro culture for conservation and to
obtain higher degree of antibacterial efficacy as well.
Keywords: In vitro culture, Therapeutic claim, Folklore medicine, In situ/ex-situ conservation.
INTRODUCTION

References

REFERENCES
1. Tsuchiya H, Sato M, Miyazaki T, Fujiwara S, Tanigaki S, Ohyama M, et al. Comparative study on the antibacterial activity of phytochemical flavanones against methicillin-resistant Staphylococcus aureus. J Ethnopharmacol 1996;50(1):27-34.
2. Mason TL, Wasserman BP. Inactivation of red beet beta glucan synthase by native and oxidized phenolic compounds. Phytochemistry 1987;26:2197-202.
3. Ya C, Gaffney SH, Lilley TH, Haslam E. Carbohydrate – polyphenol complexation. In: Hemingway RW, Karchesy JJ, editors. Chemistry and Significance of Condensed Tannins. New York: Plenum Press; 1988. p. 553.
4. Scortichini M, Pia RM. Preliminary in vitro evaluation of the antimicrobial activity of terpenes and terpenoids towards Erwinia amylovora (Burrill). J Appl Bacteriol 1991;71:109-12.
5. Gören N, Woerdenbag HJ, Bozok-Johansson C. Cytotoxic and antibacterial activities of sesquiterpene lactones isolated from Tanacetum praeteritum subsp. praeteritum. Planta Med 1996;62(5):419-22.
6. Sharma BD, Vyas MS. Ethnobotanical studies on the ferns and fern-allies of Rajasthan. Bull Bot Surv India 1985;27:90-1.
7. Banerjee RD, Sen SP. Antibiotic activity of pteridophytes. Econ Bot 1980;34(3):284-98.
8. Samy RP, Ignacimuthu S. Antibacterial activity of some folklore medicinal plants used by tribals in Western Ghats of India. J Ethnopharmacol 2000;69(1):63-71.
9. Gutmann L, Billot-Klein D, Williamson R, Goldstein FW, Mounier J, Acar JF, et al. Mutation of Salmonella paratyphi A conferring cross-resistance to several groups of antibiotics by decreased permeability and loss of invasiveness. Antimicrob Agents Chemother 1988;32(2):195-201.
10. Mohanasundari C, Natarajan D, Srinivasan K, Umamaheswari SA, Ramachandran A. Antibacterial properties of Passiflora foetida (L) - A common exotic medicinal plant. Afr J Biotechnol 2007;6(23):2650-3.
11. Kokate CK. Practical Pharmacognosy. 4th reprinted ed. New Delhi: Vallabh Prakasan 1999. p. 107, 124.
12. Tynan JL, Conner AJ, Macknight RC, Poulter RT. Miconazole: An effective antifungal agent for plant tissue culture. Plant Cell Tissue Organ Cult 1993;32(3):293-301.
13. Murashige T, Skoog TA. Revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 1962;15:473-97.
14. Bauer AW, Kirby MD, Sherris JC, Turck M. Antibiotic susceptibility testing by standardized single disc diffusion method. Am J Clin Pathol 1966;45:493-6.
15. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12(4):564-82.
16. Tanira MO, Bashir AK, Dib R, Goodwin CS, Wasfi IA, Banna NR. Antimicrobial and phytochemical screening of medicinal plants of the United Arab Emirates. J Ethnopharmacol 1994;41(3):201-5.
17. Ashour SM and Kheralla ZH. Study of antimicrobial activity by some medicinal plants. Egypt J Microbiol 1995;30:445-69.
18. Azu NC, Onyeagba RA, Nworie O, Kalu J. Antibacterial activity of Allium cepa Onion and Zingiber officinale (ginger) on Staphylococcus aureus and Pseudomonas aeruginosa isolated from high vaginal swap. Internet J Trop Med 2007;3(2):110-5.
19. Kandhasamy M, Arunachalam KD, Thatheyus AJ. Drynaria quercifolia (L.) J. Sm: A potential resource for antibacterial activity. Afr J Microbiol Res 2008;2:202-5.
20. Choi KT, Ahn IO, Park JC. Production of ginseng saponin in tissue culture of ginseng (Panax ginseng C. A. Mayer). Russ J Plant Physiol 1994;41:784-8.
21. Takahashi D, Fjita Y. Cosmetic materials. In: Komamine A, Misawa M, Dicosmo F, editors. Plant Cell Culture in Japan. Tokyo: CMC Co Ltd.; 1991. p. 72-8.
Statistics
182 Views | 303 Downloads
How to Cite
Padhy, R., and S. Kumar Dash. “ANTIBACTERIAL EVALUATION OF METHANOLIC RHIZOME EXTRACT FROM AN IN VIVO AND IN VITRO GROWN PTERIDOPHYTE, DRYNARIA QUERCIFOLIA (LINN.) J SMITH”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 4, July 2015, pp. 130-8, https://innovareacademics.in/journals/index.php/ajpcr/article/view/6072.
Section
Original Article(s)