FAILING OF INFORMATION TRANSMISSION BY DORSAL HIPPOCAMPUS DUE TO MICROINJECTION OF COLCHICINE IN RAT’S CORTICAL AREA 1

  • Afsaneh Naseri
  • Manizheh Karami Shahed University
  • Mohammadreza Jalali Nadoushan

Abstract

ABSTRACT
Objective: Colchicine has been introduced recently as a neurotoxin with damage effect on neurons of hippocampal cortical area 1 (CA1). Effect of
colchicine, a plant derived neurotoxin on memory retrieval was explored experimentally by means of novelty seeking task in intact Wistar rats.
Methods: The subjects were cannulated by stereotaxic apparatus at coordinates adjusted for the CA1 area. After recovery, all animals experienced
the novelty seeking paradigm using an unbiased conditioning device. First, they were habituated with the conditioned place preference (CPP)
apparatus. They were then confined in one part of the CPP box for 3 consecutive days. Finally, the animals were microinjected colchicine (1-25 μg/rat)
intra‑hippocampal CA1 prior to testing. Control group was cannulated too, but, solely injected saline (1-μl/rat, intra-CA1). The time spent in the novel
part of the device and the motivational signs of the rats were measured. Furthermore, the possible cell injury effect of the toxin on the CA1 layer was
verified.
Results: The alkaloid caused significant novelty seeking behavior in the experimental animals though did not show a significant effect on the
compartment entering. The destruction effect of the neurotoxin on the treated rats’ dendrites spines was evidenced.
Conclusion: Based on this finding the information transmission by dorsal hippocampal pyramidal cells may impair with an administration of
neurotoxin colchicine, intra-CA1.
Keywords: Colchicine, Memory retrieval, Novelty seeking behavior, Cortical area 1, Pyramidal cell layer.

Author Biography

Manizheh Karami, Shahed University
Dept of Biology

References

REFERENCES
1. Mundy WR, Tilson HA. Neurotoxic effects of colchicine. Neurotoxicology 1990;11(3):539-47.
2. Goldschmidt RB, Steward O. Preferential neurotoxicity of colchicine for granule cells of the dentate gyrus of the adult rat. Proc Natl Acad Sci U S A 1980;77(5):3047-51.
3. Correia JJ, Lobert S. Physiochemical aspects of tubulin-interacting antimitotic drugs. Curr Pharm Des 2001;7(13):1213-28.
4. Lockwood AH. Molecules in mammalian brain that interact with the colchicine site on tubulin. Proc Natl Acad Sci U S A 1979;76(3):1184-8.
5. McNaughton BL, Barnes CA, Meltzer J, Sutherland RJ. Hippocampal granule cells are necessary for normal spatial learning but not for spatially-selective pyramidal cell discharge. Exp Brain Res 1989;76(3):485-96.
6. Lee I, Kesner RP. Differential roles of dorsal hippocampal subregions in spatial working memory with short versus intermediate delay. Behav Neurosci 2003;117(5):1044-53.
7. Karami M, Riahi N, Nadoushan MR. Injection of colchicine intra-hippocampal cortical area 1 enhances novelty seeking behavior. Indian J Pharmacol 2013;45(3):274-7.
8. Paxinos G, Watson C. The Rat Brain in Stereotaxic Coordinates. 5th ed. Digital version. Ontario: Academic Press, Harcourt Brace Jovanovich Publisher; 2005.
9. Karami M, Zarrindast MR, Sepehri H, Sahraei H. Role of nitric oxide in the rat hippocampal CA1 area on morphine-induced conditioned place preference. Eur J Pharmacol 2002;449(1-2):113-9.
10. Panda D, Goode BL, Feinstein SC, Wilson L. Kinetic stabilization of microtubule dynamics at steady state by tau and microtubule-binding domains of tau. Biochemistry 1995;34:11117-27.
11. Kumar A, Dogra S, Prakash A. Neuroprotective effects of Centella asiatica against intracerebroventricular colchicine-induced cognitive impairment and oxidative stress. Int J Alzheimers Dis 2009; pii: 972178.
12. Giuditta A, Chun JT, Eyman M, Cefaliello C, Bruno AP, Crispino M. Local gene expression in axons and nerve endings: The glia-neuron unit. Physiol Rev 2008;88(2):515-55.
13. Priel A, Tuszynski JA, Woolf NJ. Neural cytoskeleton capabilities for learning and memory. J Biol Phys 2010;36(1):3-21.
14. Csillik B, Knyihár E, Elshiekh AA. Degenerative atrophy of central terminals of primary sensory neurons induced by blockade of axoplasmic transport in peripheral nerves. Experientia 1977;33(5):656‑7.
15. Bensimon G, Chermat R. Microtubule disruption and cognitive defects: Effect of colchicine on learning behavior in rats. Pharmacol Biochem Behav 1991;38(1):141-5.
16. Nakagawa Y, Nakamura S, Kaśe Y, Noguchi T, Ishihara T. Colchicine lesions in the rat hippocampus mimic the alterations of several markers in Alzheimer’s disease. Brain Res 1987;408(1-2):57-64.
17. Laursen B, Mørk A, Kristiansen U, Bastlund JF. Hippocampal P3-like auditory event-related potentials are disrupted in a rat model of cholinergic degeneration in Alzheimer’s disease: Reversal by donepezil treatment. J Alzheimers Dis 2014;42(4):1179-89.
18. Gajate C, Barasoain I, Andreu JM, Mollinedo F. Induction of apoptosis in leukemic cells by the reversible microtubule-disrupting agent 2-methoxy-5-(2’,3’,4’-trimethoxyphenyl)-2,4,6-cycloheptatrien-1 -one: Protection by Bcl-2 and Bcl-X(L) and cell cycle arrest. Cancer Res 2000;60(10):2651-9.
19. Wesson DW, Donahou TN, Johnson MO, Wachowiak M. Sniffing behavior of mice during performance in odor-guided tasks. Chem Senses 2008;33(7):581-96.
20. Eigsti OJ, Dustin P Jr. Colchicine in Agriculture, Medicine, Biology and Chemistry. Ames, Iowa: The Iowa State College Press; 1955.
21. Rieder CL, Palazzo RE. Colcemid and the mitotic cycle. J Cell Sci 1992;102 (Pt 3):387-92.
22. Nakayama T, Sawada T. Involvement of microtubule integrity in memory impairment caused by colchicine. Pharmacol Biochem Behav 2002;71(1-2):119-38.
23. Partida-Sánchez S, Garibay-Escobar A, Frixione E, Parkhouse RM, Santos-Argumedo L. CD45R, CD44 and MHC class II are signaling molecules for the cytoskeleton-dependent induction of dendrites and motility in activated B cells. Eur J Immunol 2000;30(9):2722-8.
24. Walsh TJ, Schulz DW, Tilson HA, Schmechel DE. Colchicine-induced granule cell loss in rat hippocampus: Selective behavioral and histological alterations. Brain Res 1986;398(1):23-36.
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How to Cite
Naseri, A., M. Karami, and M. Jalali Nadoushan. “FAILING OF INFORMATION TRANSMISSION BY DORSAL HIPPOCAMPUS DUE TO MICROINJECTION OF COLCHICINE IN RAT’S CORTICAL AREA 1”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 4, July 2015, pp. 160-3, https://innovareacademics.in/journals/index.php/ajpcr/article/view/6228.
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