GAS CHROMATOGRAPHY-MASS SPECTROMETRY/MASS SPECTROMETRY ANALYSIS OF TERMINALIA CATAPPA L. NUT AND ANTIMICROBIAL ASSAY

  • KRISHNAVENI M
  • Krishna Kumari G
  • Kalaivani M
  • Ragina Banu C

Abstract

ABSTRACT
Objective: The present study was carried out with the main objective of studying Terminalia catappa nut phyto-constituents through GC-MS/MS, as
well as its antibacterial, antifungal activities.
Methods: The T. catappa nut collected was shade dried and extracted with ethanol, the obtained ethanol extract was used for the whole study- GCMS/
MS, antimicrobial activity.
Results: The results of GC-MS/MS showed 12 peaks. Among the 12 peaks obtained, the highest peak area percent of 48.58 for Propane, 1,1-diethoxyand
24.36% for t-Butyl hydrogen phthalate followed by 3-Isopropoxy-1,1,1,7,7,7-hexamethyl-3,5,5-tris (trimethylsiloxy) tetrasiloxane showing 12.80
as peak area percent and all the other compound was found to be moderate in peak area percent which was in the range of 3.52-1.10 except β-l-
Arabinopyranoside methyl, and Cyclopropanecarboxylic acid, oct-3-en-2-yl ester as its peak area percent was very low showing 0.08 and 0.13. The
antibacterial activity was good with Escherichia coli when compared to Staphylococcus aureus likewise, the antifungal activity was good with Candida
albicans on comparison with Aspergillus niger.
Conclusion: The naturally occurring non –nutrient plant chemicals called as phytochemicals especially phenolics – a natural antioxidant contained
in T. catappa nut would have contributed for its antibacterial, antifungal properties by interfering with the phospholipid bilayer of the cell membrane
and destroying the enzymes required for cellular processes ensuring its therapeutic potential. The variation in therapeutic potential depends on the
nutrient content of the soil and other climatic conditions. This confirms the need, validity for phytochemical characterization via analytical methods.
Keywords: Antimicrobial, Analytical, Gas chromatography-mass spectrometry/MS, Terminalia sp.

Author Biography

KRISHNAVENI M

Department of Biochemistry

Senir scale

References

REFERENCES
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Table 1: Components in ethanol extract of Terminalia catappa Nut
S.No.
RT
Name of the compound
MF
MW
PA%
1
4.74
Propane, 1,1‑diethoxy‑
C7H16O2
132
48.58
2
6.10
1H‑Pyrazole, 4,5‑dihydro‑3‑methyl‑1‑propyl‑
C7H14N2
126
2.93
3
8.36
3‑Isopropoxy‑1,1,1,7,7,7‑hexamethyl‑3,5,5‑tris (trimethylsiloxy) tetrasiloxane
C18H52O7Si7
576
12.80
4
8.72
Pentanoic acid
C5H10O2
102
2.87
5
9.91
β‑l‑Arabinopyranoside, methyl
C6H12O5
164
0.08
6
16.34
Dodecanoic acid, ethyl ester
C14H28O2
228
1.10
7
19.32
8‑Methyloctahydrocoumarin
C10H16O2
168
0.81
8
25.20
t‑Butyl hydrogen phthalate
C12H14O4
222
24.36
9
29.31
Cyclopropanecarboxylic acid, oct‑3‑en‑2‑yl ester
C12H20O2
196
0.13
10
30.95
tert‑Butyl 2‑aminophenylcarbamate ditms
C17H32N2O2Si2
352
3.52
11
32.34
Stigmastan‑3,5‑diene
C29H48
396
1.30
12
35.14
Cholane‑5,20 (22)‑diene‑3b‑phenoxy
C30H42O
418
1.53
MF: Molecular formulae, MW: Molecular weight, PA: Peak area percent
Fig. 1: Chromatogram
Table 2: Antimicrobial activity of Terminalia catappa Nut
Plant part used
Microbes tested
Zone of inhibition (mm)
Terminalia catappa Nut.
Escherichia coli
7
Staphylococcus aureus
6.5
Aspergillus niger
11
Candida albicans
18
170
Asian J Pharm Clin Res, Vol 8, Issue 4, 2015, 168-170
Krishnaveni et al.
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6. Krishnaveni M, Krishna Kumari G, Ragina Banu C, Kalaivani M. GC-MS/MS analysis of phytochemicals in Terminalia catappa L, antimicrobial assay. Indo Am J Pharm Res 2015;5(3):1250-4.
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How to Cite
M, K., K. Kumari G, K. M, and R. Banu C. “GAS CHROMATOGRAPHY-MASS SPECTROMETRY/MASS SPECTROMETRY ANALYSIS OF TERMINALIA CATAPPA L. NUT AND ANTIMICROBIAL ASSAY”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 4, July 2015, pp. 168-70, https://innovareacademics.in/journals/index.php/ajpcr/article/view/6279.
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