HISTOCYTO-ARCHITECHTURAL CHANGES OF WISTER RATS SPLEEN EXPOSED TO PHOTOGRAPHIC FIXER EFFLUENT
We investigated the effect of sub chronic low dose of radiographic fixer effluent on the cyto-architecture of Wister rat spleen. Objective: The extensive use of fixer chemicals in radiographic / radiological laboratories and the attendant discharge in to the environment of the fixer effluent especially in the developing world necessitated this study. Methods: Twentyfive (25) Wister rats that have not been used for any experiment were divided into three groups namely group 1 with 5 rats which served as control and received only water, group 2 had 10 rats and received 200mg/kg (8% LD50) of fixer effluent, while group 3 with 10 rats received 400mg/kg (16% LD50) of fixer effluent. The fixer effluents were orally administered daily for the 28 days study period. The rats were given free access to feeds and water. Two weeks study: mid way, on the 14th day of the study period (short term), 5 Wister rats each from group 2 and 3 which received (200mg/kg) and (400mg/kg) administration of fixer effluent respectively were anaesthetized, sacrificed and their spleens were harvested fixed in 10% formalin saline solution awaiting further histological preparations for short-term investigation. Four weeks study: On the 28th day of the study period (medium term), the rats in control group, and the remaining 5 rats each from groups 2 and 3 were anaesthetized and sacrificed while their spleens were collected fixed in 10% formalin saline solution for histological preparations and analysis. Results: Short term administration of (200mg/kg) fixer effluent showed inflammatory cells in the red and white pulp. The Medium term administration of (200mg/kg) fixer effluent showed infiltration of inflammatory cells in the red and white pulp and equally in the venous sinuses between the sinusoids and the parenchyma of the red pulp. Also, necrosis was recorded in the red pulp parenchyma. Short term administration of 400mg/kg fixer effluent to the rats showed slightly enlarged red and white pulp with inflammation and moderate necrosis of parenchyma of the red pulp. There was also distortion in the germinal center and trabecular. Medium term administration of 400mg/kg fixer effluent to the rats caused moderately enlarged red and white pulp with severe necrosis and lymphocytic infiltrations in the red pulp parenchyma and also infiltrations in the venous sinuses between the sinusoids and parenchyma of the red pulp. Conclusion, the fixer effluent caused deleterious effects to the cellular structure of the rat spleen.
Key words: Fixer effluent, Wister rats, Sub chronic, Sinusoids, Inflammation, lymphocytic infiltration.
1 Sowerby, A.L.M. (ed.). Dictionary of Photography: A Reference Book for Amateur and Professional Photographers. London: life Books Ltd. 1961 pp. 324â€“326.
2 Mebius, RE; Kraal, G. "Structure and function of the spleen". Nature reviews Immunology 2005; 5 (8): 606â€“616.
3 Swirski FK, Nahrendorf M, Etzrodt M, Wildgruber M, Cortez-Retamozo V, Panizzi P, Figueiredo JL, Kohler RH, Chudnovskiy A, Waterman P, Aikawa E, Mempel TR, Libby P, Weissleder R, Pittet MJ. Identification of splenic reservoir monocytes and their deployment to inflammatory sites. Science 2009; 325 (5940): 612 â€“ 626.
4 Jia, T; Pamer, EG. â€œImmunology, Dispensible but not irrelevantâ€. Science 2009; 325 (5940): 549â€“550.
5 Dietrich Lorke. A new approach to practical acute toxicity testing. Arch Toxicol 1983; 54: 275-287
6 Environmental Protection Agency (EPA). Effects of Hydrazine on organs and Tissues. 1992.
7 Mayden & D. J.A. Goldsmith. â€œSodium thiosulfate: new hope for the treatment of calciphylaxis,â€Seminars in Dialysis 2010; 23 (3): 258â€“262.
8 Gard E, Marwa. A. Abass. Differential effects of alprazolam and clonazepam on the immune system and blood vessels of non stressed male albino rats. Interdiscip Toxicol 2011; 4 (3): 132- 142.
9 Vladimir N Voloshin, Vladimir G Koveshnikov, Irina Voloshina. The morphology of the spleen in adult Albino rats after whole body exposure to low level toluene. Int. J. Anat Research 2014; 2 (2): 421-430.
10 Mohammad Shahz Chohan, Uruzj\Zehra, Saad Khilji, Mohammad Tahir Fahim Haider Jafari. Paraquate induced toxicity in of Albino mice. Ann. Inst. Med. Science 2011; 7 (1): 6-9
11 Grigoletto Radiographic processing effluents management. Radiol Bras 2011; 44(5):301â€“307.
12 Mansee T, Himanshu G, Dipty Singh, Ipseeta M, Ujjwala M, Geeta V, Joshi D S Histological and ultra structural effects of nanaparticles on rat testis following 90 days (chronic study) of repeated oral administration. Journal of nonotechnology 2014; 12 (1): 42-48
13 NTP Toxicology and Carcinogenesis Studies of Boric Acid (CAS No. 10043-35-3) in B6C3F1 Mice (Feed Studies). Tech Rep Ser 1987; Oct (324): 1-126
14 Turkez H and Geyikoglu F (2010). Boric acid: a potential chemoprotective agent against aflatoxin B (1) toxicity in human blood. Cytotechnogy 2010; 62 (2): 157-165
15 World Health Organization. Environmental Health Criteria 68: Hydrazine. Geneva, Switzerland. 1987.
16 Jaeschke Hartmurt (2002).Redox considerations in hepatic injury and inflammations. Antioxidants and redox signaling 2002; 4: 699 â€“ 700.
17 Sayed S, Ashan N, Kato M, Ohgami N, Rashid A, Akhand A A (2015) Protective effect of Phyllanthus emblica leaf extract on sodium arsenite-mediated adverse effects in mice. Nagoya J Med Science 2015; 77 (1-2): 145-153.
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