ANTIOXIDANT ACTIVITY AND HEPATOPROTECTIVE POTENTIAL OF BALANITES ROXBURGHII FRUITS
Objectives: The objective of the present study was to evaluate antioxidant activity and hepatoprotective potential of a hydroalcoholic extract of
Balanites roxburghii (BR) fruits.
Methods: Extraction was performed by triple maceration using ethanol:water as a solvent. The extracts were vaporated using rotavapor. Then
antioxidant capacities were tested using superoxide, hydroxyl, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. Determination of hepatoprotective
model was performed by thiocetamide induced liver models.
Results: The hydroalcoholic extract of BR fruits produced a dose-dependent percentage inhibition on tested free radicals, i.e., superoxide anion
(50% inhibition concentrations [IC50] value 190.50 Î¼g/ml), hydroxyl radical (IC50 value 266.30 Î¼g/ml), and DPPH radical (IC50 value 175 Î¼g/ml). After
treatment with silymarin and hydroalcoholic extract of BR in respective groups, they had showed good protection against thioacetamide induced liver
Conclusion: The results of the present investigation clearly indicate the free radical scavenging activity and hepatoprotective potential of a
hydroalcoholic extract of BR fruits and this activity is comparable with that of the standard drugs ascorbic acid and silymarin.
Keywords: Balanites roxburghii, Antioxidant activity, Hepatoprotective activity, Ascorbic acid.
1. Halliwell B. Free radicals, antioxidants, and human disease: Curiosity, cause, or consequence? Lancet 1994;344:721-4.
2. Nadkarni AK, Nadkarni KM. Indian Materia Medica. Bombay: Popular Prakashan; 1976. p. 1166.
3. Chopra RN, Nayar SL, Chopra IC. Glossary of Indian Medicinal Plants. New Delhi: CSIR; 1956. p. 92.
4. Kirtikar KR, Basu BD. Indian Medicinal Plants. Deheradun: International Book Distributors; 1933. p. 31823-4.
5. McCord JM, Fridovich I. Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J Biol Chem 1969;244:6049-55.
6. Elizabeth K, Rao MN. Oxygen radical scavenging activity of curcumin. Int J Pharm 1990;58:237-40.
7. Braca A, Fico G, Morelli I, De Simone F, TomÃ¨ F, De Tommasi N. Antioxidant and free radical scavenging activity of flavonol glycosides from different Aconitum species. J Ethnopharmacol 2003;86(1):63-7. 8. Viswanath KM, Queseshi AA, Ramachandra Settee S. Hepatoprotective activity of flowers of Calotropis aprocera in paracetamol induced hepatic injury in rats. Scientific Abstracts 57 I.P.C. EP.44:261; 2005.
9. Reitman S, Frankel S. Determination of serum glutamate oxaloacetate and glutamate pyruvic acid transaminase. Am J Clin Pathol 1957;28:56-63.
10. King EJ, Armstrong AR. Determination of serum and bile phosphatase activity. Can Med Assoc J 1957;31:56-63.
11. Jendrassik L, GrÃ³f P. Simplified photometric methods for the determination of Blutbilirubins . Biochem J 1938;297:81-9.
12. Rao BG, Rao YV, Rao TM. Hepatoprotective and antioxidant capacity of Melochia corchorifolia extracts. Asian Pac J Trop Med 2013;6(7):537-43.
13. Rao TM, Rao GB, Rao YV. Antioxidant activity of Spilanthes acmella extracts. Int J Phytopharmacol 2012;3(2):216-20.
14. Ethadi S, Pragada R, Battu G. Evaluation of anti-inflammatory and hepatoprotective activities of different extracts of Cleome chelidonii root in albino rats. Int J Pharm Bio Sci 2013;4(4):111-9.
15. El-Gazzar UB, El-Far AH, Abdel Maksoud HA. The ameliorative effect of Phoenix dactylifera extract on carbon tetrachloride hepatotoxicity in New Zealand rabbits. J Appl Sci Res 2009;5(9):1082-7.
16. Poole A, Leslie GB. A Practical Approach to Toxicological Investigation. Cambridge: Cambridge University Press; 1989. p. 44-86.
17. Ringler DH, Dabich L. Haematology and clinical biochemistry. In: Barker HJ, Lindsey JR, Weisbroth SH, editors. The Laboratory Rat. Vol. I. London: Academic Press; 1979. p. 105-18.
18. Khatua S, Roy T, Acharya K. Antioxidant and free radical scavenging capacity of phenolic extract from Russula laurocerasi. Asian J Pharm Clin Res 2013;6(4):156-60.
19. Paloi S, Acharya K. Evaluation of antioxidative activity and chemical composition of ethanolic extract from Amanita vaginata (Bull.) Lam.: An in vitro study. Asian J Pharm Clin Res 2014;7(2):88-92.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.