COMMIPHORA MUKUL EXTRACT AND GUGGULSTERONE EXHIBIT ANTITUMOUR ACTIVITY THROUGH INHIBITION OF CYCLIN D1, NF-Κß AND INDUCTION OF APOPTOSIS IN ORAL CANCER CELLS

  • Vijaya Bharti 1.All india Institute of Medical Sciences 2. Singhania University, Jhunjhunu, Rajasthan
  • Umesh D Gupta JALMA Institute of Leprosy and Other Mycobacterial Diseases, Agra
  • Satya N Das All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110029

Abstract

ABSTRACT
Comiphora mukul, a promising medicinal plant and its constituent Guggulsterone (GS) is used in Ayurveda since decades. This study was aimed to
investigate the anticancer potential of C. mukul and GS on oral cancer cell lines (SCC-4, KB). MTT assay was used to determine tumour cell proliferation,
propidium iodide labeling and annexin V- binding, followed by flow cytometry was used to determine cell cycle and apoptosis of tumor cells after
treatment. Expression of regulatory proteins such as NF-κß, cyclin D1, p53 and vascular endothelial growth factor was determined by western blot.
C. mukul and GS significantly inhibited tumor cell growth, caused cell cycle arrest and apoptosis in both tumor cells. Such activities appeared to be
due to inhibition of NF-κß, cyclin D1and restoration of p53. Overall our data suggests that C. mukul and GS may be developed as chemopreventive and
chemotherapeutic drug for oral cancer.
Keywords: Commiphora mukul, Oral cancer, Antitumor, Cell cycle, Apoptosis, NF-κß, Cyclin D1, P53.

Author Biographies

Vijaya Bharti, 1.All india Institute of Medical Sciences 2. Singhania University, Jhunjhunu, Rajasthan
Department of Biotechnology
Umesh D Gupta, JALMA Institute of Leprosy and Other Mycobacterial Diseases, Agra
Head, Experimental Animal Facility
Satya N Das, All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110029
Professor, Department of Biotechnology

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How to Cite
Bharti, V., U. D. Gupta, and S. N. Das. “COMMIPHORA MUKUL EXTRACT AND GUGGULSTERONE EXHIBIT ANTITUMOUR ACTIVITY THROUGH INHIBITION OF CYCLIN D1, NF-Κß AND INDUCTION OF APOPTOSIS IN ORAL CANCER CELLS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 4, July 2015, pp. 291-5, https://innovareacademics.in/journals/index.php/ajpcr/article/view/6713.
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