MICROEMULSION FORMULATION OF ALOE VERA GEL AND APIUM GRAVEOLENS ETHANOL EXTRACT FOR OPTIMIZING HAIR GROWTH PROMOTION
Objective: Follicular penetration of hair promotion substances to the bulge region is needed to potentiate cell proliferation and hair shaft lengthening.
Nano-size globule is one of important delivery systems applied for hair follicle targeting. This research aims to explore the effect of microemulsion
on hair promoting activities of a mixture of hydrophilic substances of Aloe vera gel and semipolar substances of Apium graveolens ethanolic extract.
Methods: The clear water in oil (W/O) and oil in water (O/W) microemulsions were developed by constructing phase diagram of various ratios
of Crodamol GTCC, Croduret 50 SS, glycerin/alcohol, and water. A. vera (1%) and celery extract (2%) were incorporated during microemulsion
preparation. The microemulsions were applied over shaved skin areas of the backs of Wistar rats, and the average length of 10 hair samples was
determined at day 7, 14, and 21 compared to the un-treated area. The weight of hair/cm2 area was also analyzed at day 21. Hair growth promotion of
the microemulsions was compared to the other groups of rats applied by A. vera in water (AVW), celery extract in water and oil (CEW and CEO), and
combination of A. vera and celery extract in water (AVCEW).
Result: The W/O and O/W microemulsion consisted of globules at the average size of 61.7 and 81.5 nm, respectively, which showed stable size
following six cycles of freeze-thaw. The W/O microemulsion increased hair growth dramatically started at day 7 (220%) and day 14 (275%) and
slowed-down at day 21 (163%) with similar profile shown by AVW (74.5, 65.8, 31.7%, respectively). On the contrary, the hair growth promotion of
O/W microemulsion delayed to day 14 but maintained till day 21 (143.7, 214.5, 171.9 at day 7, 14, and 21, respectively) with similar profile shown
by CEW and CEO. Hair densities were also doubled in the areas treated by both microemulsion types than in the areas treated by a combination of
AVCEW in water.
Conclusion: Incorporation of hydrophilic and semipolar substances of hair growth promoter in nano-size globules of W/O and O/W microemulsions
lead to pilosebaceous targeting and optimize the growth promotion activities.
Keywords: Aloe vera, Apium graveolens, Water in oil microemulsion, Oil in water microemulsion, Hair growth, Pilosebaceous.
1. Shelton RM. Aloe vera. Its chemical and therapeutic properties. Int J Dermatol 1991;30(10):679-83.
2. Boudreau MD, Beland FA. An evaluation of the biological and toxicological properties of Aloe barbadensis (Miller) Aloe vera. J Environ Sci Health 2006;24(1):103-54.
3. Aburjai T, Natsheh FM. Plants used in cosmetics. Phytother Res 2003;17(9):987-1000.
4. Syamsuhidayat SS. Inventaris Tanaman Obat Indonesia. Vol. I. Jakarta, Balitbang: Kesehatan, Depkes RI; 1991. p. 62, 476.
5. Huh S, Lee J, Jung E, Kim SC, Kang JI, Lee J, et al. A cell-based system for screening hair growth-promoting agents. Arch Dermatol Res 2009;301(5):381-5. 6. Wosicka H, Cal K. Targeting to the hair follicles: Current status and potential. J Dermatol Sci 2010;57(2):83-9.
7. Knorr F, Lademann J, Patzelt A, Sterry W, Blume-Peytavi U, Vogt A. Follicular transport route â€“ Research progress and future perspectives. Eur J Pharm Biopharm 2009;71(2):173-80.
8. Lawrence MJ, Rees GD. Microemulsion-based media as novel drug delivery systems. Adv Drug Deliv Rev 2000 6;45(1):89-121.
9. Li L, Jiang H, Wu H, Zeng S. Simultaneous determination of luteolin and apigenin in dog plasma by RP-HPLC. J Pharm Biomed Anal 2005;37(3):615-20.
10. Li LP, Jiang HD. Determination and assay validation of luteolin and apigenin in human urine after oral administration of tablet of Chrysanthemum morifolium extract by HPLC. J Pharm Biomed Anal 2006;41(1):261-5.
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