A REVIEW ON MATRIX ASSISTED LASER DESORPTION/INOZATION MASS SPECTROSCOPY

  • Ch Aparna* Andhra University
  • N Madhavi Latha Andhra University
  • P Supriya Andhra University
  • D Gowrisankar

Abstract

ABSTRACT
Matrix assisted laser desorption ionization mass spectroscopy (MALDI-MS) is the most important technique of MS to analyze polymer systems. It is
a special case of MS using specific sample preparation methods and low fluence laser desorption to create the analyte ions. This technique is based
upon an ultraviolet absorbing matrix. The matrix and the polymer are mixed at a molecular level in an appropriate solvent. The solvent helps prevent
aggregation of the polymer. The sample matrix mixture is placed on the sample probe tip, under vacuum conditions; the solvent is removed, leaving cocrystallized
polymer molecules homogenously dispersed within matrix molecules. When the pulsed laser beam is tuned to the appropriate frequency,
the energy is transferred to the matrix which is partially vaporized, carrying intact polymer into the vapor phase and charging the polymer chains in
the linear time of flight (TOF) analyzer. This review includes the detailed information of MALDI-MS, MALDI-TOF.
Keywords: Matrix assisted laser desorption ionization mass spectroscopy, Principle, Sample preparation techniques, Matrix assisted laser desorption
ionization - time of flight, Matrix assisted laser desorption ionization - mass spectrometric imaging, Applications.

Author Biographies

Ch Aparna*, Andhra University

Department of Pharmaceutical Analysis and Quality Assurance,

m.pharmacy,

Andhra university, pin code-530003

N Madhavi Latha, Andhra University

Department of Pharmaceutical Analysis and Quality Assurance,

m.pharmacy,

Andhra university, pin code-530003

P Supriya, Andhra University

Department of Pharmaceutical Analysis and Quality Assurance,

m.pharmacy,

Andhra university, pin code-530003

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How to Cite
Aparna*, C., N. Madhavi Latha, P. Supriya, and D. Gowrisankar. “A REVIEW ON MATRIX ASSISTED LASER DESORPTION/INOZATION MASS SPECTROSCOPY”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 5, Sept. 2015, pp. 28-33, https://innovareacademics.in/journals/index.php/ajpcr/article/view/6968.
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Review Article(s)