QUALITATIVE PHYTOCHEMICAL SCREENING AND EVALUATION OF IN-VITRO ANTIOXIDANT ACTIVITY OF CALLICARPA ARBOREA ROXB, AN ETHNOMEDICINAL PLANT OF MIZORAM, NORTHEAST INDIA
Objective: The objective of the present study was to evaluate the phytochemical constitution and antioxidant activity of methanolic extract of dried leaves of Callicarpa arborea Roxb, an ethnomedicinal plant of Mizoram, Northeast India
Methods: Qualitative analysis of phytochemical constituents was performed by the well known tests protocol available in the literature on the methanolic leaves extract of the plant. Antioxidant activity was studied through DPPH and reducing power using BHT and ascorbic acid respectively as standards. The total phenolic and total flavonoid content were determined using Gallic acid and Quercetin respectively as standards.
Results: The phytochemical screening revealed the presence of saponin, steroids, glycosides, flavonoids and tannins. The methanol extract exhibited IC50 values of 2.393 Âµg/ml in DPPH assay. The reducing power of the extract was lower than that of the standard ascorbic acid. The total phenolic content and flavonoid content was 130.6 mg/g and 83.9 mg/g of gallic acid and quercetin equivalent respectively.
Conclusion: The results suggest that methanolic leaves extract of Callicarpa arborea has promising antioxidant activity and could serve as potential source of natural antioxidants.Â
Keywords: Callicarpa arborea, Phytochemicals, Antioxidant activity, Diphenyl-1-picrylhydrazyl, Phenol and flavonoid.
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