INHIBITORY EFFECT OF ETHANOLIC ROOT EXTRACT OF COIX LACHRYMAJOBI ON HYALURONIDASE AND L-AMINO ACID OXIDASE OF NAJA NAJA AND DABOIA RUSSELLI VENOM
Objective: This study was carried out to investigate the efficacy of inhibitory effect of ethanolic root extract (ERE) of plant Coix lachrymajobi (Poaceae) on hyaluronidase and L-amino acid oxidase (LAAO) of Daboia russelii and Naja naja venom.Â
Methodology: The ethanolic root extract of C. lachrymajobi is used to treat snake bite victims by traditional healers as folk medicine for centuries, was tested in-vitro to determine its ability to inhibit D. russelli and N. naja hyaluronidase and LAAO activities.Â In-vitro studies were carried out with different doses of ERE.
Results & Discussion: which effectively neutralized hyaluronidase and LAAO activities of both D. russelli and N. naja venom.Â ERE at doses of 2000Âµg successfully inhibited D. russelli (50 Âµg) hyaluronidase activity by 67.04% and LAAO activity by 77.86%. Â The hyaluronidase activity of N. naja venom (50 Âµg) was inhibited by 71.83% and LAAO activity was reduced by 74.83%.Â From the results, It is evident that the ERE of Coix lachrymajobi effectively neutralizes important toxic enzymes of the D. russelli and Naja naja venoms.Keywords: Daboia russelii; Naja naja; Coix lacryma-jobi; Hyaluronidase; L-amino acid oxidase
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