THE PROTECTIVE EFFECT OF THYMOQUINONE AGAINST LEAD ACETATE INDUCED DNA DAMAGE AND ALTERATIONS IN TUMOR INITIATION GENES
objective: Several pollutants represent a significant ecological and public health concern due to their toxicity and their ability to accumulate in living
organisms in which lead is one of them. The present investigation was designated to assess the modulating effect of thymoquinone (TQ) against lead
acetate (LA) toxicity.
Methods: Several endpoints were considered to design this study such as: The gene expression of tumor initiation genes (cytochrome P450 3A
[CYP3A], cyclooxygenase 2 [COX2], BAX and Bcl
), DNA damage and alterations in the levels of glutathione (GSH), lipid oxidation (malondialdehyde
[MDA]), and protein oxidation (protein carbonyl [PC]) in male rats. About 60 male rats were used in this study which allocated in six groups (10 animal
each) and treated with LA (200 mg/kg diet), TQ (5 and 10 mg/kg b.wt.), and LA + TQ.
Results: The results revealed that LA induced significant DNA damage and alteration in the expression of CYP3A, COX2, BAX, and Bcl
as well as
induced changes in GSH content and MDA and PC levels in male rats. Meanwhile, TQ was decreased significantly the toxic effect of LA in male rats
which decreased the alterations in the gene expression and DNA damage as well as GSH content and MDA and PC levels.
Conclusion: The results suggested that TQ treatment confers protection against toxicity inflicted by LA and support the contention that TQ protection
is achieved by its ability as a scavenger for free radicals generated by LA.
Keywords: Thymoquinone, Lead acetate, Gene expression, DNA damage, Rats.
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