PROTEOMIC ANALYSIS OF DIFFERENT EXTRACTS FROM AMARANTH (AMARANTHUS TRICOLOR) GRAINS.

  • REEMA SRIVASTAVA BANARAS HINDU UNIVERSITY
  • BIJOY K. ROY BANARAS HINDU UNIVERSITY

Abstract

Amaranth seed has a high nutritional value due to the balanced composition of its protein fractions (i.e albumins, globulins, prolamins and glutelins). These were isolated by sequential extractions. The amount of albumins, globulins, prolamins and glutelins were 2.90, 2.20, 0.47 and 5.73 g/100g of seed flour. Upon electrophoresis, albumins were found to be most polymorphic, while prolamins were made up of fewer and less abundant components. Gel electrophoresis analysis revealed the occurrence of glutelins as the major proteins in the seed. Amaranth protein isolates were prepared by (a) extraction at different alkaline pHs and precipitation at pH 5 and (b) extraction at pH 9 and precipitation at different pHs. The isolates were compared with protein fractions. Acidification at pH 5 and precipitation at lower pHs lead to denaturation of the proteins which was partially reversed by returning to pH 7.

Author Biographies

REEMA SRIVASTAVA, BANARAS HINDU UNIVERSITY

DEPARTMENT OF BOTANY AND ASSTT PROF

BIJOY K. ROY, BANARAS HINDU UNIVERSITY

DEPTT OF BOTANY AND PROF

References

1. Mujica A, and Jacobsen SE. The genetic resources of Andean grain amaranths (Amaranthus caudatus L., A. cruentus and A. hypochondriacus L.) in America. Plant genetic resources Newsletter .2003 ;133: 41-44.
2. Costea MG, Waines and Sanders A. Structure of the pericarp in some Amaranthus L. (Amaranthaceae) species and its taxonomic significance. Aliso. 2001b; 20: 51–60.
3. Dehmer KJ. Molecular diversity in the genus Amaranthus. In: Knüpffer H, Ochsmann J, Schriften zu Genetischen Ressourcen, Band 22. Bonn. Rudolf Mansfeld and Plant Genetic Resources. 2003. 208–215.
4. Costea M, Weaver SE and Tardif FJ. The biology of Canadian weeds. 130. Amaranthus retroflexus L., A. powellii S. Watson and A. hybridus L. Canadian Journal of Plant Science. 2004; 84: 631–668.
5. Gorinstein S, Delgado-Licon E, Pawelzik E, Heriyati Permandy H, Weisz M, Trakhtenberg S. Characterization of soluble amaranth and soybean protein based on fluorescence, hydrophobicity, electrophoresis, amino acid analysis, circular dichroism, and differential scanning calorimetry measurements. Journal of Agricultural and Food Chemistry. 2001; 49: 5595–5601.
6. Abugoch, LE, Martinez NE and Anon MC. Influence of the extracting solvent upon the structural properties of amaranth (Amaranthus hypochondriacus) glutelin. Journal of Agriculture and Food Chemistry. 2003; 51: 4060–4065.
7. Tomoskozi S, Gyenge L, Pelceder A, Varga J Abonyi T and Lasztity R, Functional properties of protein preparations from amaranth seeds in model system, Eur Fods Res Technol. 2008; 226: 1343-1348.
8. Martinez EN and Anon MC. Composition and structural characterization of Amaranth protein isolates. An electrophoretic and calorimetric study. J.Agric. Food Chem. 1996 ;44: 2523-2530.
9. Scilingo AA, Ortiz SEM, Martinez EN, Anon MC. Amaranth protein isolates modified by hydrolytic and thermal treatments. Relationship between structure and solubility. Food Res Int. 2002; 35: 855-862.
10. Lowry OH, Ronserough NJ, Farr AL and Randell RJ. Protein measurement with the folin reagent. J. Biol. Chem. 1951 ;193: 265-275.
11. Sammour RH. Proteins of linseed (Linum usitatissimum L.), extraction and characterization by electrophoresis. Bot. Bull. Acad. Sin. 1999; 40:121-126.
12. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 ;227: 680-685.
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How to Cite
SRIVASTAVA, R., and B. K. ROY. “PROTEOMIC ANALYSIS OF DIFFERENT EXTRACTS FROM AMARANTH (AMARANTHUS TRICOLOR) GRAINS.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 6, no. 7, Aug. 2013, pp. 37-39, https://innovareacademics.in/journals/index.php/ajpcr/article/view/77.
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