STUDY OF ANTI-ANAEMIC EFFECT OF SCHREBERA SWIETENIOIDES ROXB. IN RAT MODELS
Objective: Ethnobotanical survey of Schrebera swietenioides Roxb revealed the bark of the tree to be useful in anemia. The objective of this study was
to study the ant-anemic effect of methanolic extract of root bark of S. swietenioides Roxb. against phenylhydrazine induced anemic rat model.
Methods: The methanolic extracts of Leaf, Stem bark, and Root bark were prepared by soxhlation. Phytochemical analysis of the extracts was performed
using standard testing procedures. The total phenolic content (TPC) of Schrebera leaf extract, Schrebera stem bark extract and Schrebera root bark
extract (SRE) was determined by Folinâ€“Ciocalteu method. Hemolytic anemia was induced in male Wistar rats by intraperitoneal administration
of phenylhydrazine HCl (PHZ) at doses of 40 mg/kg body weight/day for 3 consecutive days. Anemic rats were orally treated with SRE at doses of
200 and 350 mg/kg body wt/day. The rats were analyzed for hematological parameters such as hemoglobin (Hb), red blood cell count (RBC) and
hematocrit or packed cell volume (PCV) on day 4 and 14.
Results: Phytochemical screening of the extracts indicated the presence of carbohydrates, saponins, sterols, polyphenols, tannins, and flavonoids.
Folinâ€“Ciocalteu method of testing for TPC demonstrated SRE to be rich in total phenols with a value of 266 mg GAE/g of dry extract. Anemia was
induced successfully in Groups II, III, IV, and V, which was indicated by a mean reduction of 51.6% in RBC count; 53.85% in Hb content, and 54.9% in
PCV. Analysis of hematological parameters on day 14 showed that SRE significantly (p<0.05) improved Hb, RBC count, and PCV at a dose of 350 mg/kg
Conclusion: This study, not only substantiates the folklore use of the root bark of S. swietenioides, but also suggests its inclusion in the treatment of
anemia as it exhibited significant anti-anemic activity.
Keywords: Schrebera swietenioides, Anemia, Hematological parameters, Phenyl hydrazine, Hematocrit.
anaemia and measures taken to curb the problem. Food Nutr
2. Holden J, Acomb C. Anaemia. In: Walker R, Whittlesea C, editors.
Clinical Pharmacy and Therapeutics. New York: Churchill Livingstone;
2007. p. 699-701.
3. Powers A, Silberstein LE. Autoimmune haemolytic anaemia. In:
Hoffman R, editor. Haematology: Basic Principles and Practice. 5
Philadelphia, PA., USA: Elsevier/Churchill Livingstone; 2009.
4. Beutler E. Haemolytic anaemia due to chemical and physical agents.
In: Beutler E, Coller BS, Lichtman MA, Kipps TJ, Seligsohn U,
editors. Williams Hematology. 6th ed. New York: McGraw-Hill; 2001.
5. Kozlov VA, Zhuravkin IN, Coleman RM, Rencricca NJ. Splenic
plaque-forming cells (PFC) and stem cells (CFU-s) during acute
Asian J Pharm Clin Res, Vol 8, Issue 5, 2015, 307-310
Pingali et al.
phenylhydrazine-induced enhanced erythropoiesis. J Exp Zool
6. Yeshoda KM. Phenylhyrazine anaemia in rats. Curr Sci 1942;11:360-3.
7. Silja VP, Samitha Verma K, Mohanan KV. Ethnomedicinal plant
knowledge of the mullu kuruma tribe of Wayanad district of Kerala.
Indian J Tradit Knowl 2008;7(4):604-12.
8. Omolo OJ, Chhabra SC, Nyagah G. Determination of iron content in
different parts of herbs used traditionally for anaemia treatment in East
Africa. J Ethnopharmacol 1997;58(2):97-102.
9. Falade CO, Oladoyinbo SO, Elemile TT, Ajayi IO, Fawole OI,
Oladepo O, et al. How well equipped are healthcare facilities to manage
childhood malaria? The situation in selected local government areas in
South Western Nigeria. Afr J Med Med Sci 2006;35(3):329-35.
10. Oladiji AT, Jacob TO, Yakubu MT. Anti-anaemic potentials of
aqueous extract of Sorghum bicolor (L.) moench stem bark in rats.
J Ethnopharmacol 2007;111(3):651-6.
11. Agbor GA, Oben JE, Ngogang JY. Haematinic activity of Hibiscus
cannabinus. Afr J Biotechnol 2005;4:833-7.
12. Diallo A, Gbeassor M, Vovor A, Eklu-Gadegbeku K, Aklikokou K,
Agbonon A, et al. Effect of Tectona grandis on phenylhydrazineinduced
anaemia in rats. Fitoterapia 2008;79(5):332-6.
13. Kirtikar KR, Basu BD. Indian Medicinal Plants. Vol. II. Dehradun:
International Book Publisher; 1993. p. 1531-2.
14. Manda H, Rao BK, Yashwant, Kutty GN, Swarnkar SK. Antioxidant,
anti-inflammatory and antipyretic activities of ethyl acetate fraction of
ethanolic extract of Schrebera swietenioides roxb. Root. Intl J Toxicol
Pharm Res 2009;1(1):7-11.
15. Bagali RS, Jalalpure SS. Evaluation of antidiabetic and antioxidant
effect of Schrebera swietenioides fruit ethanolic extract. Pharm Lett
16. Harbone JB. Pytochemical Methods: A Guide to Modern Techniques of
Plant Analysis. 2nd ed. London: Chapman and Hall; 1988. p. 55-6.
17. Alhakmani F, Kumar S, Khan SA. Estimation of total phenolic content,
in-vitro antioxidant and anti-inflammatory activity of flowers of
Moringa oleifera. Asian Pac J Trop Biomed 2013;3(8):623-7.
18. Ghosh MN. Toxicity Studies, Fundamentals of Experimental
Pharmacology. 2nd ed. Calcutta: SC and RC Book Agencies; 1984.
19. Mir AH, Sexena M, Malla MY. An acute oral toxicity study of
methanolic extract from Tridex procumbens in Sprague Dawleyâ€™s rats
as per OECD guidelines 423. Asian J Plant Sci Res 2013;3(1):16-20.
20. Rifkind RA, Danon D. Heinz body anemia â€“ An ultrastructural study. I.
heinz body formation. Blood 1965;25:885-96.
21. Suzuki Y. The development of a sensitive micronucleus test: An
in vitro method using cultured bone marrow cells. Jikeikai Med J
22. Adamson JW, Longo DL. Hematologic alterations. In:
Braumwald E, Fauci AS, Kasper DL, Hauser SL, Longo DL,
Jameson JL, editors. Harrisonâ€™s Principles of Internal Medicine.
New York: McGraw Hills; 2001.
23. Biswas S, Bhattacharyya J, Dutta AG. Oxidant induced injury of
erythrocyte-role of green tea leaf and ascorbic acid. Mol Cell Biochem
24. Turashkar A, More S, Sheikh R, Gadhpayle J, Bhongde SI.
Inhibitory potential of Picrorhiza kurroa royle ex. benth extracts on
phenylhydrazine induced reticulocytosis in rats. Asian J Pharm Clin
25. Ndem JI, Otitoju O, Akpanaiabiatu MI, Uboh FE, Uwah AF,
Edet OA. Haematoprotective property of Eremomastax speciosa
(Hochst.) on experimentally induced anaemic wistar rats. Ann Biol Res
26. Waghmare AN, Tembhurne SV, Sakarkar DM. Anti-anaemic potential
of Murraya koenigii fruit extracts in phenylhydrazine induced anaemic
rats. IJAPR 2015;6(05):124-7.
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