ANTIDIABETIC ACTIVITY OF ETHANOLIC EXTRACT OF EUGENIA POLYANTHA WIGHT LEAF FROM INDONESIA IN DIABETIC RAT WISTAR STRAIN INDUCED BY ALLOXAN

Abstract

Objective: The purpose of this study is to determine the antidiabetic activity of 96% ethanolic extract of Eugenia polyantha wight leaves.

Methods: Twenty-five male Wistar rats were divided into 5 groups. Group I was negative control (aquabidest),group II was positive control (Glibenclamide 0.45 mg/kg), group III, IV, and V were treated by the 96% ethanolic extract of leaves of Eugenia polyantha wight with doses of 312,5; 625; and 1250 mg/kg respectively. Before the treatment, All rats were induced by  alloxan with dose of 150 mg / kg by intra peritoneal. On 3^rd day after induction, rats with blood glucose level> 200 mg/dL were treated by drugs or extract appropriate to the group. On 10^th day, after blood sampling, all rats were killed and the pancreas were taken for histophatology examination. The blood glucose level on 10^th day was tested by  Kruskall- Wallis test followed by the Mann-Whitney with confidence level  95%.

Results:  The 96% ethanolic extract of leaves of Eugenia polyantha wight at doses of 312.5 mg/kg, 625 mg/kg and 1250 mg/kg body weight can reduce blood glucose levels in rat induced by alloxan. The 96% ethanolic extract of Eugenia polyantha wight at dose of 1250 mg/kg body weight potentially regenerate Langerhans  cell  induced by alloxan. This extract has potentials as antidiabetic agent

Conclusion: This study shows that the 96% ethanolic extract of  Eugenia polyantha wight can be developed as an antidiabetic agent