AMELIORATING EFFECTS OF PYRROLOQUINOLINE QUINONE (PQQ) ON PTU INDUCED OXIDATIVE DAMAGE IN MICE KIDNEY

Abstract

Objective: The present study undertaken to investigate the hitherto unknown potential of pyrroloquinoline quinine (PQQ) has been evaluated in ameliorating PTU induced oxidative damage in adult mice kidney.

Material and Method: Five groups of 7 each healthy male mice were established. Group I animals receiving simple drinking water served as control, whereas those of group II, III, IV and V received either PTU (0.05percent in drinking water) alone  for 5 weeks or PTU +  three different concentrations of PQQ (1, 5 and 10 mg/kg/d, i.p. for 6 days), after which alterations in tissue lipid peroxidation (LPO) and in enzymatic activities of superoxide dismutase (SOD) and catalase (CAT) and in glutathione (GSH) content were evaluated in kidney. Simultaneously, concentrations of serum glucose, total cholesterol, glutamate oxaloacetate transaminase (SGOT), creatinine and urea were measured in serum.

Result: PTU administration enhanced the tissue LPO, serum SGOT, total cholesterol, creatinine and urea with a parallel decrease in serum glucose and tissue antioxidants such as SOD and CAT. However, when PTU treated animals received PQQ; these adverse effects were ameliorated, as it reduced the LPO, with a parallel increase in cellular antioxidants.

Conclusion: Out of three different doses of PQQ (1, 5 and 10 mg/kg), 10 mg/kg body weight was found to be the most effective and antiperoxidative in nature; Findings from this study revealed for the first time, that PQQ has the potential to ameliorate PTU-induced oxidative damage in kidney of mice, indicating the possible beneficial effect of the test compound in regulating PTU induced oxidative damages.

 

Keywords: PQQ, PTU, Kidney, Antioxidant, SGOT, Mice