ANTIBACTERIAL ACTIVITY OF TABERNAEMONTANA DIVARICATA (APOCYNACEAE) SECONDARY METABOLITES CAPPED SILVER AND GOLD NANOPARTICLES
Objective: To study the antibacterial activity of Tabernaemontana divaricata (Apocynaceae) secondary metabolites capped silver nanoparticles
(SNPs) and gold nanoparticles (AuNPs).
Methods: In the present investigation, SNPs and AuNPs were synthesized using an aqueous extract of T. divaricata leaves. Leaf aqueous extract
was mixed with 1 mM silver nitrate and chloroauric acid for the biosynthesis of nanoparticles, and the same was analyzed using ultraviolet-visible
(UV-Vis) spectrophotometer and particle size analyzer. The antibacterial activity of SNPs and AuNPs was determined against various bacterial cultures
including laboratory isolates using the agar well diffusion method.
Results: The results recorded from UV-Vis spectrum supported the biosynthesis and characterization of SNPs and AuNPs. The SNPs when compared
to AuNPs, showed the highest antibacterial activity against Gram-positive and Gram-negative bacteria.
Conclusion: The present study envisions on the biosynthesis of SNPs from T. divaricata plant which are emerging as antibacterial therapy in modern
Keywords: Antibacterial activity, Gold nanoparticles, Silver nanoparticles, Tabernaemontana divaricata.
of silver nanoparticles using Tabernaemontana divaricata and its
cytotoxic activity against MCF-7 cell line. Int J Pharm Pharm Sci
2. Pushpa B, Latha KP, Vaidya VP, Shruthi A, Shweatha A. Phytochemical
analysis and antimicrobial evaluation of leaves extract of
Tabernaemontana coronaria. J Chem Pharm Res 2012;4:3731-3.
3. Gopinath SM, Suneetha TB, Anand S. Evaluation of antibacterial
activity of Tabernaemontana divaricata leaves against the causative
organisms of Bovine mastitis. Int J Res Phytochem Pharmacol
4. Poornima K, Krishna R, Gopalakrishnan K. Toxicological evaluation
of ethanolic extract of Tabernaemontana coronaria. Asian J Trop Dis
5. Savithramma N, Rao LM. Antimicrobial activity of silver nanoparticles
synthesized by using medicinal plants. Int J Chem Technol Res
6. Xu C, Lin X, Ren H, Zhang Y, Wang S, Peng X. Analysis of outer
membrane proteome of Escherichia coli related to resistance to
ampicillin and tetracycline. Proteomics 2006;6(2):462-73.
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