IN VIVO ACUTE TOXICITY, ANTIBACTERIAL, ANTIAQUATIC FUNGAL, ANTHELMINTHIC ACTIVITY OF LACTOBACILLUS PLANTARUM KP894100 AND LACTOBACILLUS ACIDOPHILUS KP942831
Objective: This study observed the antibacterial, antifungal (aquatic and pathogenic fungi), and anthelmintic properties of Lactobacillus plantarum
(LP) KP894100 and Lactobacillus acidophilus (LC) KP942831, isolated from a dairy product.
Methods: Lactic acid bacteria traditionally used to improve human health. For in vitro antibacterial, antifungal, and antihelminthic studies of
intracellular cell-free extract (ICFE) from LP and LC were produced separately by using filtration methods. ICFE was freeze-dried then resuspended
in citrate phosphate buffer. This ICFE is further used for the antimicrobial and anthelmintic assay. In the antimicrobial assay, ICFE were tested against
pathogenic bacteria, i.e., Bacillus subtilis, Escherichia coli, Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas
stutzeri, Pseudomonas aeruginosa, and aquatic fungal species including: Aspergillus clavatus, Pythium aphanidermatum Saprolegnia parasitica,
Candida albicans, Aspergillus fumigatus, Fusarium oxysporum, Alternaria alternata, Curvularia sp., Mucor sp., Rhizopus stolonifer, Aspergillus niger, and
Penicillium chrysogenum. In the anthelmintic assay, young stages of Pheretima posthuma (Indian earthworms) were used. For the analysis of acute
toxicity assay different graded doses (100, 250, 500, 1000 mg/kg bw) of ICFE of LP and LC was administered in Wistar albino rats, respectively, and
the control group received distilled water.
Result: The ICFE of both Lactobacillus strains showing strong antibacterial and weak antifungal activity against aquatic fungi except, S. parasitica, and
C. albicans. The ICFE shows 100% paralysis and killing efficacy against P. posthuma in 48-72 hrs. In the acute toxicity test, LP and LC did not produce
any toxic signs or death at the maximum concentration 1000 mg/kg bw.
Conclusion: ICFE of LP and LC possess anthelmintic activity against P. posthuma with only strong antibacterial activity. Both the lactobacillus strains
have strong antibacterial activity and have potential activity against P. posthuma helminthes.
Keywords: Antimicrobial, Antifungal, Aquatic fungi, Anthelmintic activity, Pheretima posthuma.
of Thespesia Lampas (Cav.). Asia J Pharm Clin Res 2009;2(2):2-3.
2. Bundy DA. Immunoepidemiology of intestinal helminthic infections 1.
The global burden of intestinal nematode disease. Trans R Soc Trop
Med Hyg 1994;88(3):259-61.
3. Tagboto S, Townson S. Antiparasitic properties of medicinal plants and
other naturally occurring products. Adv Parasitol 2001;50:199-295.
4. Sondhi SM, Shahu R, Archana M. Anthelmintic activity of Platycladus
orientalis leaves extract. Indian Drugs 1994;31(7):317-20.
5. Joerger RD. Alternatives to antibiotics: Bacteriocins, antimicrobial
peptides and bacteriophages. Poult Sci 2003;82(4):640-7.
6. Temmerman R, Pot B, Huys G, Swings J. Identification and antibiotic
susceptibility of bacterial isolates from probiotic products. Int J Food
7. Reis JA, Paula AT, Casarotti SN, Penna AL. Lactic acid bacteria
antimicrobial compounds: Characteristics and applications. Food Eng
8. de Moreno de LeBlanc A, Matar C, PerdigÃ³n G. The application of
probiotics in cancer. Br J Nutr 2007;98 Suppl 1:S105-10.
9. Thirabunyanon M, Hongwittayakorn P. Potential probiotic lactic
acid bacteria of human origin induce antiproliferation of colon
cancer cells via synergic actions in adhesion to cancer cells and
short-chain fatty acid bioproduction. Appl Biochem Biotechnol
10. Maeda M, Shibata A, Biswas G, Korenaga H, Kono T, Itami T, et al.
Isolation of lactic acid bacteria from kuruma shrimp (Marsupenaeus
japonicus) intestine and assessment of immunomodulatory role of a
selected strain as probiotic. Mar Biotechnol (NY) 2014;16(2):181-92.
11. Udhayashree N, Senbagam D, Senthilkumar B, Nithya K, Gurusamy R.
Production of bacteriocin and their application in food products. Asia
Pac J Trop Biomed 2012;2:406-10.
12. Rhee SJ, Lee JE, Lee CH. Importance of lactic acid bacteria in Asian
fermented foods. Microb Cell Fact 2011;10 Suppl 1:S5.
13. Laparra JM, Sanz Y. Interactions of gut microbiota with functional food
components and nutraceuticals. Pharmacol Res 2010;61(3):219-25.
14. Rafter J. Lactic acid bacteria and cancer: Mechanistic perspective. Br J
Nutr 2002;88 Suppl 1:S89-94.
15. Report of a Joint FAO/WHO Working on Group on Drafting Guidelines
for the Evaluation of Probiotics in the Food. London, Ontario, Canada;
16. Wadher KJ, Mahore JG, Umekar MJ. Probiotics: Living medicines
in health maintenance and disease prevention. Int J Pharm Bio Sci
17. Nirmal SA, Malwadkar G, Laware RB. Anthelmintic activity of
Pongamia glabra. Songklanakarin J Sci Tech 2007;29(3):755-7.
18. Herich R, Levkut M. Lactic acid bacteria, probiotics and immune
system. Vet Med 2002;47(6):169-80.
19. De Man JC, Rogosa M, Sharp ME. A medium for the cultivation of
Lactobacilli. J Appl Bacteriol 1960;23(1):130-5.
20. Chiu YH, Lu YC, Ou CC, Lin SL, Tsai CC, Huang CT, et al.
Lactobacillus plantarum MYL26 induces endotoxin tolerance
phenotype in Caco-2 cells. BMC Microbiol 2013;13:190.
21. Zhou JS, Shu Q, Rutherfurd KJ, Prasad J, Gopal PK, Gill HS.
Acute oral toxicity and bacterial translocation studies on potentially
probiotic strains of lactic acid bacteria. Food Chem Toxicol 2000;
22. Wolf BW, Garleb KA, Ataya DG, Casas IA. Safety and tolerance of
Lactobacillus reuteri in healthy adult male subjects. Microbiol Ecol
Health Dis 1995;8:41-50.
23. Hladikova Z, Smetankova J, Greif G, Greifova M. Antimicrobial
activity of selected lactic acid cocci and production of organic acids.
Acta Chimica Slovaca 2012;1(5):80-5.
24. Oliveira RB, de L Oliveira A, GlÃ³ria MB. Screening of lactic acid
bacteria from vacuum packaged beef for antimicrobial activity. Braz J
25. Magnusson J, SchnÃ¼rer J. Lactobacillus coryniformis subsp.
Coryniformis strain Si3 produces a broad-spectrum proteinaceous
antifungal compound. Appl Environ Microbiol 2001;67(1):1-5.
26. Ahirwal L, Mehta A, Mehta P, John J, Singh S. Anthelmintic
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