POTENTIAL ALPHA GLUCOSIDASE INHIBITOR FROM SELECTED ZINGIBERACEAE FAMILY

  • Patonah Hasimun Bandung School of Pharmacy
  • I Ketut Adnyana School of Pharmacy, ITB
  • Rizka Valentina Bandung School of Pharmacy
  • Euis Lisnasari bandung school of pharmacy

Abstract

ABSTRACT
Objective: This study aimed to evaluate Zingiberaceae family as potential sources of alpha-glucosidase inhibitors (AGIs).
Methods: A 16 species of selected Zingiberaceae family were macerated by ethanol 96%. The extracts were filtered and concentrated by rotary
evaporator. Phytochemical analysis carried out on the dry extract. AGI was assayed in 50 mM phosphate buffer at pH 6.8 with 5 mM p-nitrophenyl-αD-glucopyranoside
as
the substrate.
Alpha-glucosidase
activity
was
determined
by
measuring the release
of the yellow
p-nitrophenol
at 400
nm
using
a
spectrophotometer
UV-Vis,
and the percentage
of inhibition was
calculated.
Results and Conclusion: The Zingiberaceae family showed IC
against alpha-glucosidase varies range from 28.4 µg/ml to 269.2 µg/ml. Curcuma
longa L., Zingiber cassumunar Roxb., Curcuma heyneana Val. Et van zijp, Curcuma xanthorrhiza Roxb., Zingiber ottensii Val., showed the highest
inhibition against alpha-glucosidase, with IC
50
50
28.4, 49.0, 78.2, 78.9, and 79.0 µg/ml, respectively. The Zingiberaceae family is potential sources as an
alternative medicine to treated diabetes mellitus.
Keywords: Zingiberaceae, Alpha-glucosidase inhibitor, Diabetes mellitus, Phytochemistry content.

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How to Cite
Hasimun, P., I. K. Adnyana, R. Valentina, and E. Lisnasari. “POTENTIAL ALPHA GLUCOSIDASE INHIBITOR FROM SELECTED ZINGIBERACEAE FAMILY”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 9, no. 1, Jan. 2016, pp. 164-7, https://innovareacademics.in/journals/index.php/ajpcr/article/view/9622.
Section
Original Article(s)