A COMPARATIVE STUDY OF ANTI-ANGIOGENIC ACTIVITIES OF MEDICINAL PLANTS AND ITS THERAPEUTIC POTENTIAL IN ANGIOGENESIS-DEPENDENT DISORDERS
Angiogenesis, the process of new vessel growth from pre-existing capillaries is involved in physiological and pathological process such as embryonic development, wound healing, diabetic retinopathy ,chronic inflammation, tumor growth and metastasis. Vascular endothelial growth factor (VEGF) induced angiogenesis plays an important role in the pathogenesis of diabetic retinopathy and cancer and anti VEGF factor agents are found to be effective . VEGF inhibitors from plant sources show promising result recently. So this study is designed to evaluate and compare the VEGF inhibitory effect of the herbs Osimum Sanctum, Andrographis paniculata, Syzygium cumini and Ginkgo biloba by using in vivo models.
Methods: Both Corneal neovascularization assay and Chick Chorioallantoic membrane assays were used for evaluation. The induced neovascularization was compared with control and extract treated groups.
Results: The acute and repeated dose toxicity tests showed that the extracts are safe on oral administration. And also the results showed significant antiangiogenic effect of individual extract and their combination in both in vivo models. However the combination of the extract showed excellent result (p <0.001) by reducing neovascularization in both models
Conclusion: The above results suggested that combination of the above extracts have promising future in the management of angiogenesis dependent disorders.
Key words: Osimum Sanctum, Andrographis paniculata, Syzygium cumini, Ginkgo biloba,Â Â Angiogenesis, chorioallantoic membrane, angiogenesis dependent disorders.
1. Adair TH, Montani JP.San Rafael (CA): Morgan & Claypool Life Sciences; 2010.
2. Neufeld. G,Cohen T, Gengrinovitch.S and Poltorak.Z vitch et al, Vascular endothelial growth factor (VEGF) and its receptors. FASEB J 1999;13:9-22.
3. Ann Hoeben, Bart Landuyt, Martin S. Highley, Hans Wildiers, Allan T. Van Oosterom, et al. Vascular Endothelial Growth Factor and Angiogenesis.Pharmacol Rev 2004; 56: 549-580.
4. Asahara T, Takahashi T, Masuda H, Kalka C, Chen D, et al. VEGF contributes to postnatal neovascularization by mobilizing bone marrow-derived endothelial progenitor cells. EMBO J 1999;18:3964-3972.
5. N. Gupta, S. Mansoor, A. Sharma, A. Sapkal, J. Sheth.P et al.Diabetic Retinopathy and VEGF.The Open Ophthalmology Journal 2013;7:4-10.
6. Eremina V, Quaggin SE et al.The role of VEGF-A in glomerular development and function. Curr Opin Nephrol Hypertens 2004;13:9-15.
7. Dai J, Rabie AB et al .VEGF: an essential mediator of both angiogenesis and endochondral ossification. J Dent Res 2007;86: 937-950.
8. Leung DW, Cachianes G, Kuang WJ, Goeddel DV, Ferrara N et al.Vascular endothelial growth factor is a secreted angiogenic mitogen. Science 1989;246:1306â€“1309.
9. D.C.Felmeden, A.D. Blann, G.Y.H.Lip et al.Angiogenesis; basic pathophysiology and implications for disease. Europian Heart Journal.2003;24:586-603.
10. Homayouni.M et al. Vascular Endothelial Growth Factors and Their Inhibitors in Ocular Neovascular Disorders. J Ophthalmic Vis Res 2009; 4(2):105â€“114.
11. Hicklin DJ, Ellis LM et al.Role of the vascular endothelial growth factor pathway in tumor growth and angiogenesis.J Clin Oncol 2005;23:1011-1027.
12. Soo-Jin Jeong,Wonil Koh, Eun-Ok Lee, Hyo-Jung Lee, Hyo-Jeong Lee,,Hyunsu Bae, et al.Antiangiogenic Phytochemicals and Medicinal Herbs; Phytother. Res 2010;25(1):1-10.
13. Mahapatra Arun Kumar, Nisha Kumari Ojha,Abhimanyu Kumar et al. Prospective Role of Indian Medicinal Plants in Inhibiting Vascular Endothelial Growth Factor (VEGF) mediated Pathological Angiogenesis. J Homeop Ayurv Med 2013; 2(2):121.
14. The organization for Economic â€“co-operation and Developmnt (OECD) .Test no 423.Acute Oral Toxicity-Acute toxic class method, in OECD guidelines for testing of chemicals section 4: Health effects: OECD publishing:2002
15. The organization for Economic â€“co-operation and Developmnt (OECD). Test no.408.Repeated 90 day oral toxicity study in Rodents I n OECDguidelines for testing of chemicals section 4: Health effects:OECD publishing:1998
16. Carolyn A. Staton, Stephen M. Stribbling,â€ , Simon Tazzyman, Russell Hughes, Nicola J. Brownâ€ and Claire E. Lewis et al. Current methods for assaying angiogenesis in vitro and in vivo. Int. J. Exp. Path 2004; 85: 233â€“248.
17. Zhongshu Tang, Fan Zhang, Yang Li A et al. Mouse Model of the Cornea Pocket Assay for Angiogenesis Study..Journal of Visual Experiments 2011;54:3077.
18. Nguyen M, Yoo Y.C, Folkman J et al. Quantitation of angiogenesis and antiangiogenesis in chick embryo chorioallentoic membrane.Microvascular research1994;47;37-40.
19. Ning Cheung, Ian Y. Wong ,Tien Y.,Wong et al. Ocular Anti-VEGF Therapy for Diabetic Retinopathy: Overview of Clinical Efficacy and Evolving Applications; Diabetes Care 2014;37:900â€“905.
20. Miller K, Wang M, Gralow J, et al. Paclitaxel plus bevacizumab versus paclitaxel alone for metastatic breast cancer. N Engl J Med 2007;357:2666â€“2676.
21. Jie Ma and David J. Waxman et al. Combination of Anti-angiogenesis with Chemotherapy for More Effective Cancer Treatment. Mol Cancer Ther 2008;7(12): 3670â€“3684.
22. Han-Chung Wu1, Chia-Ting Huang, and De-Kuan Chang et al. Anti-Angiogenic Therapeutic Drugs for Treatment of Human Cancer. Journal of cancer molecules. 2008;4(2):37-45.
23. S.M. Sagar MD,D. Yance MH and R.K. Wong MD et al. Natural health products that inhibit angiogenesis: a potential source for investigational new agents to treat cancerâ€”Part 1. Current Oncology.2006;13(1):14-26.
24. Gordon M. Cragg, David J et al. Newman.Plants as a source of anti-cancer agents. Journal of Ethnopharmacology 2005,100:72â€“79.
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