IN VITRO ASSESSMENT OF ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF GREEN SYNTHESIZED SILVER NANOPARTICLES FROM DIGITARIA RADICOSA LEAVES

  • Kalaiyarasu T Research scholar, Department of Biochemistry, Periyar University, Salem, Tamilnadu -11.
  • Karthi N Research scholar, Department of Biochemistry, Periyar University, Salem, Tamilnadu -11.
  • Sharmila Gowri V Research scholar, Department of Biochemistry, Periyar University, Salem, Tamilnadu -11.
  • Manju V Assistant Professor, Department of Biochemistry, Periyar University, Salem, Tamilnadu-11.

Abstract

Green metallic nanoparticles were creating a new era in the field of green nanotechnology and its applications. Methanolic leaf extract of rare, endemic medicinally important herb, Digitaria radicosa used for the synthesis of silver nanoparticles (SNPs). UV Visible spectrophotometric analysis confirmed the synthesis of green silver nanoparticles indicated by the peak observed at 442nm due to the excitation of surface plasmon resonance in the silver nanoparticles. FT-IR spectroscopic analysis showed the availability of functional groups which may involve in the silver nanoparticles synthesis. X-Ray Diffraction pattern illustrated the characteristic peaks of (111), (122), (231) facets of the centre crystalline and cubic face centred nature of silver nanoparticles. SEM analysis showed that synthesized green silver nanoparticles were of spherical in shape and size of around 90 nm. The free radical scavenging activity of silver nanoparticles were evaluated in vitro by using DPPH scavenging activity, metal chelating activity, reducing power assay and hydrogen peroxide scavenging assays. The antibacterial activity against food borne pathogens such as S. aureus and E .coli were determined by disc diffusion method. The results confirmed that these synthesized green silver nanoparticles identified to have significant in vitro antioxidant potential and good antibacterial activity.

Keywords:

Green Silver nanoparticles, SNPs, Digitaria radicosa leaf extract, UV Visible spectrophotometry, XRD, FT IR, SEM, in vitro antioxidant assays, antibacterial activity.

Author Biographies

Kalaiyarasu T, Research scholar, Department of Biochemistry, Periyar University, Salem, Tamilnadu -11.
Research scholar
Karthi N, Research scholar, Department of Biochemistry, Periyar University, Salem, Tamilnadu -11.
Research scholar
Sharmila Gowri V, Research scholar, Department of Biochemistry, Periyar University, Salem, Tamilnadu -11.
Research scholar
Manju V, Assistant Professor, Department of Biochemistry, Periyar University, Salem, Tamilnadu-11.
Assistant Professor

References

1. Gavhane J., Padmanabhan P., Kamble S.P. and Jangle S.N., Synthesis of silver nanoparticles using extract of neem leaf and Triphala and evaluation of their antimicrobial activites, Int J Pharm Bio Sci., 2012, 3(3), 88-100.
2. Balagurunathan R., Radhakrishnan M., Babu Rajendran R. and Velmurugan D., Biosynthesis of gold nanoparticles by actinomycete Streptomyces viridogens strain HM10 Indian, J. Biochem.Biophy., 2011, 48:331-335.
3. Parasharu K., Saxena and Srivastava A., Bioinspired Synthesis of Silver Nanoparticles, Digest Journal of Nanomaterials and Biostructures., 2009,Vol. 4, No.1,159-166.
4. Mukherjee P., Ahmad A., Mandal D., et al., Fungus-mediated synthesis of silver nanoparticles and their immobilization in the mycelial matrix: a novel biological approach to nanoparticle synthesis, Nano Letters., 2001,1(10),515-519.
5. Askari S., Halladj R. and Nasernejad B., Characterization and preparation of sonochemically synthesized silver–silica nanocomposites. Mater. Sci. Pol., 2009, 2, 27.
6. Gardea Torresday, J.L., Gomez, E., Peralta-Videa, J., Parsons, J.G.,Troiani, H. and Santiago., Alfalfa sprouts, a natural source for the synthesis of silver nanoparticles., Langmuir The ACS journal of surfaces and colloids., 2003, 19:1357-1361.
7. Iravani S., Green synthesis of metal nanoparticles using plants, Green Chemistry, 2011, vol. 13, no. 10, 2638–2650.
8. Korbekandi H., Iravani S, and Abbasi S.,Production of nanoparticles using organisms production of nanoparticles using organisms, Critical Reviews in Biotechnology, 2009,vol. 29, no. 4,279-306.
9. Parashar V., Parashar R., Sharma B. and A. C. Pandey., Digest Journal of Nanomaterials and Biostructures, 2009, 4(1), 45- 50.
10. Sharma V.K., Yngard R.A, Lin, Y., Silver nanoparticles: green synthesis and their antimicrobial activities. Adv. Colloid Interface Sci., 2009, 145, 83-96.
11. Chaloupka K., Malam Y. and Seifalian, AM., Nanosilver as a new generation of nanoproduct in biomedical applications. Trends Biotechnol., 2010, 28, 580-588.
12. Prow T.W., Grice J.E., Lin L.L., Faye R., Butler M., Becker W., Wurm E.M.T., Yoong C., Robertson T.A., Soyer H.P. and Roberts M.S., Nanoparticles and microparticles for skin drug delivery, Adv. Drug Deliv. Rev., 2011, 63, 470-491.
13. Chaudhry Q. and Castle L., Food applications of nanotechnologies: an overview of opportunities and challenges for developing countries, Trends Food Sci. Tech., 2011, (22), 595-603.
14. Nair R., Varghese S.H., Nair B.G., Maekawa T., Yoshida Y. and Sakthi Kumar D., Nanoparticulate material delivery to plants, Plant Sci., 2010, 179, 154-163.
15. Kelly F.M. and Johnston J.H., Colored and functional silver nanoparticle wool fiber composites, ACS Appl. Mater. Interfaces., 2011,3,1083-1092.
16. Dankovich T.A. and Gray D.G., Bactericidal paper impregnated with silver nanoparticles for point-of-use water treatment, Environ. Sci. Technol., 2011, 45, 1992 -1998.
17. Niraimathi, KL, Sudha, V, Lavanya, R, Brindha, P: Biosynthesis of silver nanoparticles using Alternanthera sessilis (Linn.) extract and their antimicrobial, antioxidant activities, Colloid. Surface B., 2013, 102, 288-291.
18. Sankar R., Karthik A., Prabu A., Karthik S., Shivashangari K.S. and Ravikumar V., Origanum vulgare mediated biosynthesis of silver nanoparticles for its antibacterial and anticancer activity, Colloid. Surface B., 2013, 108, 80-84.
19. Boca S.C., Potara M., Gabudean A.M., Juhem A., Baldeck P.L., Astilean S., Chitosan coated triangular silver nanoparticles as a novel class of biocompatible, highly effective photothermal transducers for in vitro cancer cell therapy, Cancer Lett., 2011, 31, 131-140.
20. Bar H., Bhui D.H., Sahoo P.G., Sarkar P., De P.S. and Misra A., Green synthesis of silver nanoparticles using latex of Jatrapha curcas, Colloids Surf A Physicochem Eng Asp., 2009, 339:134-139.
21. Dubey S.P., Lahtinen M. and Sillanpaa M., Tansy fruit mediated greener synthesis of silver and gold nanoparticles, Process Biochem., 2010, 45:1065-1071.
22. Sathishkumar M, Sneha K, Won S.W., Cho C.W., Kim S. and Yun Y.S., Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity, Colloids Surf B., 2009, 73:332-338.
23. Satyavani K., Ramanathan T. and Gurudeeban S., Plant Mediated Synthesis of Biomedical Silver Nanoparticles by Using Leaf Extract of Citrullus colocynthis, Res J Nanosci Nanotechnol., 2011, 1:95-101.
24. Daizy P., Mangifera Indica leaf-assisted biosynthesis of well dispersed silver nanoparticles. Spectrochimica Acta Part A., 2011, 78:327-331.
25. Hemali Padalia, Pooja Moteriya and Sumitra Chanda., Green synthesis of silver nanoparticles from marigold flower and its synergistic antimicrobial potential, Arabian Journal of Chemistry,Volume 8, Issue 5, 2015,732–741.
26. Prabhu and Poulose, Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects. International Nano Letters 2012 2:32.
27. Amit Kumar M., Abhishek K., and Uttam Chand B., Free Radical Scavenging and Antioxidant Activity of Silver Nanoparticles Synthesized from Flower Extract of Rhododendron dauricum, Nano Biomed. Eng. 2012, 4(3), 118-124.
28. Abdel-Aziz M.S., Shaheen M.S., El-Nekeety A.A. and Abdel-Wahhab M.A., Antioxidant and antibacterial activity of silver nanoparticles biosynthesized using Chenopodium murale leaf extract, Journal of Saudi Chemical Society 2013, 1234-1239.
29. Inbathamizh L, Mekalai Ponnu T, Jancy Mary E., In vitro evaluation of antioxidant and anticancer potential of Morinda pubescens synthesized silver nanoparticles, Journal of Pharmacy Research, 2013, 32-38.
30. Kim J.S, Kuk E, Yu K.N, Kim J.H, Park S.J., Lee H.J and et al Antimicrobial effects of silver nanoparticles, Nanomed. Nanotechnol. Biol. Med. 3 (2007) 95–101.
31. Feng Q.L, Wu J, Chen G.Q, Cui F.Z, Kim T.N, Kim J.O. Mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J. Biomed. Mater., 52 (2000), 662–668.
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How to Cite
T, K., K. N, S. G. V, and M. V. “IN VITRO ASSESSMENT OF ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF GREEN SYNTHESIZED SILVER NANOPARTICLES FROM DIGITARIA RADICOSA LEAVES”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 9, no. 1, Jan. 2016, pp. 297-02, https://innovareacademics.in/journals/index.php/ajpcr/article/view/9715.
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