• SOWMIYA R Professor,Department of OCAS,Alagappa University..
  • Prasanna Kumar S
  • Deepak P
  • Ramkumar R
  • Balasubramani G
  • Aiswarya D
  • Perumal P


Objectives: To investigate the antiplasmodial activity of three different solvent extracts of Sargassum tenerrimum against Plasmodium falciparum.
Methods: The seaweed species of S. tenerrimum were collected from Rameshwaram, Southeast coast of India. The collected samples were dried and
extracted with three different polaritic (hexane, acetone, and ethylacetate) solvents and tested against P. falciparum parasite strain.
Results: Acetone extract exhibited better activity than the other two extracts. The inhibitory concentration
values of acetone S. tenerrimum were
found to be 27.82 and 18.14 µg/ml at 24-48 hrs, respectively. S. tenerrimum crude extracts were subjected for the phytochemical analysis, and it
showed the presence of steroids, alkaloids, flavonoids, tannins, glycosides, amino acids, and phenol compounds. The gas chromatography-mass
spectroscopy result reveals that the presence of 10 major and minor compounds in the S. tenerrimum extract. In that, cyclotrisiloxane hexamethyl
compounds might be responsible for the effective parasite suppression.
Conclusion: It can be concluded from the present study that the acetone extract of S. tenerrimum has strong antiplasmodial activity. Furthermore, the
study has been extended to the isolation of the possible active compounds that is responsible for the antiplasmodial properties.
Keywords: Antiplasmodial assay, Different polaritic solvents, Plasmodium falciparum, Sargassum tenerrimum.



Madara AA, Ajayi AJ, Salawu AO, Tijani AY. Anti-malarial

activity of ethanolic leaf extract of Piliostigma Thonningii Schum

(Caesalpiniacea) in mice infected with Plasmodium berghei. Afr J

Biotech 2010;9(23):3475-80.

World Health Organization. World Malaria Report. Geneva,

Table 1: (Continued)




19.845 14.735

O 412 29.394 40.593

Switzerland; 2014. Available from:

Corbett Y, Herrera L, Gonzalez J, Cubilla L, Capson TL, Coley PD,

et al. A novel DNA-based microfluorimetric method to evaluate

antimalarial drug activity. Am J Trop Med Hyg 2004;70(2):119-24.

WHO. Traditional Medicine Strategy. Geneva, Switzerland: WHO/

EDM/TRM; 2002.

Smith AJ. Medical and pharmaceutical applications of seaweed natural

products: A review. J Appl Phycol 2004;16:245-62.

Jeyanthi RL, Dhanalakshmi V, Sharmila S, Merina PD. Isolation of

phenolic compounds from marine algal extracts. Res J Pharm Biol

Chem Sci 2013;4(1):38-42.

Jeyanthi RL, Dhanalakshmi V, Sharmila S, Merina PD. Bactericidal

activity of Stechospermum sp. Res J Pharm Biol Chem Sci


Vairappan CS, Daitoh M, Suzuki M, Abe T, Masuda M. Antibacterial

halogenated metabolites from the Malaysian Laurencia species.

Phytochemistry 2001;58(2):291-7.

Thirumaran G, Vijayabaskar P, Anantharaman P. Antibacterial

and antifugal activities of brown marine macroalge (Dictyota

dichotoma) from the Gulf of Mannar biosphere reserve. Environ Ecol


Sahoo D. Common Seaweed of India. New Delhi, India: International

Publishing House; 2010.

Moore GE, Gerner RE, Franklin HA. Culture of normal human

leukocytes. JAMA 1967;199(8):519-24.

Trager W. The cultivation of Plasmodium falciparum: Applications

in basic and applied research on malaria. Ann Trop Med Parasitol


Azas N, Laurencin N, Delmas F, Di GC, Gasquet M, Laget M, et al.

Synergistic in vitro antimalarial activity of plant extracts used as

traditional herbal remedies in Mali. Parasitol Res 2002;88(2):165-71.

Rasoanaivo P, Ratsimamanga Urverg S, Ramanitrhasimbola D,

Rafatro H, Rakoto Ratsimamanga A. Screening of plant extracts from

Madagascar to search for antimalarial activity and effect potentiator

chloroquine. J Ethnopharmacol 1992;64:117-26.

Waako PJ, Katuura E, Smith P, Folb P. East African medicinal plants as

a source of lead compounds for the development of new antimalarial

drugs. Afr J Ecol 2007;45(1):102-6.

Harbone JB. Phytochemical Methods: A Guide to Modern Techniques

of Plant Analysis. London: Chapman & Hall; 1973. p. 279.

Trease GE, Evans WC. Pharmacognosy. 13

ed. London: ELBS/

Bailliere Tindall; 1989. p. 772-3.


Ghannadi A, Plubrukarn A, Zandi K, Sartavi K, Yegdaneh A. Screening

for antimalarial and acetylcholinesterase inhibitory activities of some

Iranian seaweeds. Res Pharm Sci 2013;8(2):113-8.

Ravikumar S, Ramanathan G, Gnanadesigan M, Ramu A,

Vijayakumar V. In vitro antiplasmodial activity of methanolic extracts

from seaweeds of South West Coast of India. Asian Pac J Trop Med


Asian J Pharm Clin Res, Vol 9, Issue 2, 2016, 101-106

Sowmiya et al.

Ravikumar S, Jacob Inbaneson S, Suganthi P, Gokulakrishnan R,

Venkateson M. In vitro antiplasmodial activity of ethanolic extracts

of seaweed macroalgae against Plasmodium falciparum. Parasitol Res


Yang RY, Li CY, Lin YC, Peng GT, She ZG, Zhou SN. Lactones

from a brown alga endophytic fungus (No. ZZF36) from the South

China Sea and their antimicrobial activities. Bioorg Med Chem Lett


Beula MJ, Sona SM, Prasannakumar S, Ravikumar S, Kumaran R.

Variable drug bio availability of native seaweeds in India for antimalarial

therapeutics. Drug Discov 2015;10(25):93-9.

Sherman PW, Billing J. Darwinian gastronomy: Why we use spices.

Bioscience 1999;49:453-63.

Li XC, Jaeob MR, Ding Y, Agarwal AK, Ismillie T, Khan SI,

et al. Capisterones A and B which enhance fluconazole activity in

Saccharomyces cerevisiae, from the marine green alga Penicillus

capitatus. J Nat Prod 2006;69:542-6.

McPhail KL, Correa J, Linington RG, Gonzalez J, Ortega-Barría E,

Capson TL, et al. Antimalarial linear lipopeptides from a Panamanian

strain of the marine cyanobacterium Lyngbya majuscula. J Nat Prod


Linington RG, Gonzalez J, Ureña LD, Romero LI, Ortega-Barría E,

Gerwick WH. Venturamides A and B: Antimalarial constituents of

the Panamanian marine Cyanobacterium Oscillatoria sp. J Nat Prod


Cowan MM. Plant products as antimicrobial agents. Clin Microbiol

Rev 1999;12:564-82.

Rosa SD, Iodice C, Khalaghdoust M, Oryan S, Rustaiyan A. Spatane

diterpenoids from the brown algae Stoechosperum marginatum

(Dictyotaceae). Phytochem 1999;51:1009-12.

Ozdemir G, Karabay NU, Dalay MC, Pazarbasi B. Antibacterial activity

of volatile component and various extracts of Spirulina platensis.

Phytother Res 2004;18(9):754-7.

Aaronson S. Important vegetable supplements. Stable foods. In: The

Cambridge World History Food. Cambridge: Cambridge University;

p. 231-249.

Lalia S. Comparative phytochemical investigation on a variety of

marine algae from Karachi coast. Thesis Doctor of Philosophy in

Botany. Faculty of Botany. University of Karachi: Pakistan; 2003.

p. 189-202.

Balamurugan M, Ganapathy selvam G, Thinakaran T, Siva kumar K.

Biochemical study and GC-MS analysis of Hypnea musciformis (Wulf)

Lamouroux. Am-Eurasian J Sci Res 2013;8(3):117-23.

Murata T, Miyase T, Muregi FW, Naoshima-Ishibashi Y, Umehara K,

Warashina T, et al. Antiplasmodial triterpenoids from Ekebergia

capensis. J Nat Prod 2008;71:167-74.



How to Cite

R, S., P. K. S, D. P, R. R, B. G, A. D, P. P, and R. . S. “IN VITRO ANTIPLASMODIAL ACTIVITY OF NATIVE INDIAN SEAWEED SARGASSUM SP.”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 2, Mar. 2016, pp. 101-6,



Original Article(s)