A VALIDATED ANALYTICAL HPLC METHOD FOR THE QUANTIFICATION OF LINCOMYCIN HYDROCHLORIDE IN BULK AND SOLID DOSAGE FORM

  • P. Rajeev Kumar Department of Pharmaceutics, School of Pharmacy, PRIST University, Manamai-Nallur, Chennai, Tamilnadu, India
  • Rekha Rajeevkumar Department of Pharmaceutical Analysis, School of Pharmacy, PRIST University, Manamai-Nallur, Chennai, Tamilnadu, India

Abstract

Objective: Develop a simple isocratic reverse phase high performance liquid chromatographic method (RP-HPLC) and validate for the determination of lincomycin hydrochloride (LMH) in bulk and pharmaceutical preparations.

Methods: RP-HPLC quantification was carried out by using fine pack SIL RPC18 column. The mobile phase (methanol: water) was pumped at a flow rate of 1 ml/min in the ratio of 90:10 v/v and the eluents were monitored at 254 nm.

Results: The retention time of the drug was 3.73 min and produced at a linear response in the concentration range of 5-25µg/ml. The percentage RSD was found to be below 2%. The LOD and LOQ were found to be 0.854µg/ml and 0.258µg/ml respectively.

Conclusion: Validation of the method was performed for precision, accuracy, linearity, ruggedness, specificity and sensitivity to conform to ICH guidelines for valuation for analytical methods.

Keywords: Lincomycin hydrochloride (LMH), RP-HPLC, Validation, Recovery, Tablet dosage

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How to Cite
Kumar, P. R., & Rajeevkumar, R. (2017). A VALIDATED ANALYTICAL HPLC METHOD FOR THE QUANTIFICATION OF LINCOMYCIN HYDROCHLORIDE IN BULK AND SOLID DOSAGE FORM. International Journal of Applied Pharmaceutics, 9(3), 42-44. https://doi.org/10.22159/ijap.2017v9i3.17327
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