A VALIDATED ANALYTICAL HPLC METHOD FOR THE QUANTIFICATION OF LINCOMYCIN HYDROCHLORIDE IN BULK AND SOLID DOSAGE FORM


P. Rajeev Kumar, Rekha Rajeevkumar

Abstract


Objective: Develop a simple isocratic reverse phase high performance liquid chromatographic method (RP-HPLC) and validate for the determination of lincomycin hydrochloride (LMH) in bulk and pharmaceutical preparations.

Methods: RP-HPLC quantification was carried out by using fine pack SIL RPC18 column. The mobile phase (methanol: water) was pumped at a flow rate of 1 ml/min in the ratio of 90:10 v/v and the eluents were monitored at 254 nm.

Results: The retention time of the drug was 3.73 min and produced at a linear response in the concentration range of 5-25µg/ml. The percentage RSD was found to be below 2%. The LOD and LOQ were found to be 0.854µg/ml and 0.258µg/ml respectively.

Conclusion: Validation of the method was performed for precision, accuracy, linearity, ruggedness, specificity and sensitivity to conform to ICH guidelines for valuation for analytical methods.


Keywords


Lincomycin hydrochloride (LMH), RP-HPLC, Validation, Recovery, Tablet dosage

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About this article

Title

A VALIDATED ANALYTICAL HPLC METHOD FOR THE QUANTIFICATION OF LINCOMYCIN HYDROCHLORIDE IN BULK AND SOLID DOSAGE FORM

Keywords

Lincomycin hydrochloride (LMH), RP-HPLC, Validation, Recovery, Tablet dosage

DOI

10.22159/ijap.2017v9i3.17327

Date

01-05-2017

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol 9, Issue 3, 2017 Page: 42-44

Online ISSN

0975-7058

Statistics

191 Views | 49 Downloads

Authors & Affiliations

P. Rajeev Kumar
Department of Pharmaceutics, School of Pharmacy, PRIST University, Manamai-Nallur, Chennai, Tamilnadu, India
India

Rekha Rajeevkumar
Department of Pharmaceutical Analysis, School of Pharmacy, PRIST University, Manamai-Nallur, Chennai, Tamilnadu, India
India


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