DEVELOPMENT AND CHARACTERIZATION OF NOVEL HERBAL FORMULATION (POLYMERIC MICROSPHERES) OF SYZYGIUM CUMINI SEED EXTRACT


Ranu Biswas, Kalyan Kumar Sen

Abstract


Objective: The purpose of the present investigation was to develop and characterize a novel herbal formulation (polymeric microspheres) of Syzygium cumini seed extract.

Methods: The extract-loaded microspheres using biological macromolecule ethyl cellulose (EC) was prepared by o/w emulsion solvent evaporation technique using polyvinyl alcohol (PVA) emulsifier. The effect of various process and formulation variables (stirring speed, evaporation time, drug/polymer ratio and organic/aqueous phase ratio) on the properties of microspheres was evaluated.

Results: Micromeritic properties indicated good flow properties, and scanning electron microscopy (SEM) confirmed the spherical nature of the prepared microspheres. The particle size and entrapment efficiency were varied between 34.25 to 176.25 µm and 10.51 to 42% depending upon the variables. All the formulations showed minimal drug release in an acidic environment (pH 1.2) confirming the prevention of drug release in the stomach and enteric nature of the polymer. Sustained drug release has been observed in alkaline dissolution media (pH 7.4) after 12 h of drug release study except for formulation F7 which contains a lower concentration of polymer. The fourier transform infrared spectroscopy (FTIR) analysis indicated the compatibility of the extract with the polymer. The absence of extract-polymer interaction was indicated by the differential scanning calorimetry (DSC) thermogram. x-ray diffraction (XRD) analysis revealed the amorphous nature of the extract in the microspheres which in pure form exhibits a crystalline structure.

Conclusion: The findings of this present study suggest that microsphere formulation was a promising carrier for novel delivery of herbal drugs.


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References


Parth S, Verma S, Misri P. Global need for novel herbal drug formulations. Int J Pharm Phytopharm Res 2016;8 Suppl 9:1535-44.

Kumar A, IIavarasan R, Jayachandran T, Deecaraman M, Aravindan P, Padmanabhan N, et al. the Anti-diabetic activity of Syzygium cumini and its isolated compound against streptozotocin-induced diabetic rats. J Med Plants Res 2008;2 Suppl 9:246-9.

Farswan M, Mazumder PM, Parcha V. Modulatory effect of an isolated compound from Syzygium cumini seeds on biochemical parameters of diabetes in rats. Int J Green Pharm 2014;3:128-33.

Banerjee A, Maji B, Mukherjee S, Chaudhuri K, Seal T. In vitro anti-diabetic and antioxidant activities of ethanol extract of Tinospora sinensis. Int J Curr Pharm Res 2017;9 Suppl 2:42-7.

Prabakaran C, Shanmugavel G. Antidiabetic activity and phytochemical constituents of syzygium cumini seeds in puducherry region, South India. Int J Pharm Phyto Res 2017;9 Suppl 7:985-9.

Mukherjee PK, Harwansh RK, Bhattacharyya S. Bioavailability of herbal products: approach toward improved pharmacokinetics. Evidence-Based Validation Herbal Med 2015;10:217-45.

Kumar K, Raj AK. Development and evaluation of floating microspheres of curcumin. Trop J Pharm Res 2012;11 Suppl 5:713-9.

Hazra M, Mondal DD, Mondal T, Bhuniya S, Ghosh M. Designing polymeric microparticulate drug delivery system for hydrophobic drug quercetin. Saudi Pharm J 2015;23 Suppl 4:429-36.

Akash MSH, Iqbal F, Raza M, Rehman K, Ahmed S, Shahzad Y, et al. Characterization of ethyl cellulose and hydroxypropyl methylcellulose microspheres for controlled release of flurbiprofen. J Pharm Drug Delivery Res 2013;2 Suppl 1:3-10.

Guo W, Quan P, Fang L, Cun D, Yang M. Sustained release donepezil loaded PLGA microspheres for injection: preparation, in vitro and in vivo study. Asian J Pharm Sci 2015;10:405-14.

Nighute AB, Bhise SB. Preparation and evaluation of rifabutin loaded polymeric microspheres. Res J Pharm Technol 2009;2 Suppl 2:371-4.

Natarajan V, Natarajan K, Madan B, Sehgal PK. Formulation and evaluation of quercetin polycaprolactone microspheres for the treatment of rheumatoid arthritis. J Pharm Sci 2010;100:195-204.

Subedi G, Shrestha AK, Shakya S. Study of effects of different factors in formulation of micro and nanospheres with solvent evaporation technique. Open Pharm Sci J 2018;5:182-95.

Sharma M, Kohli S, Dinda A. In vitro and in vivo evaluation of repaglinide loaded floating microspheres prepared from different viscosity grades of HPMC polymer. Saudi Pharm J 2015;23:675-82.

Chitnis KS, Palekar SB, Koppar DR, Mestry DY. Evaluation of Syzygium cumini Linn. Seed formulations available in the market using spectrophotometric and chromatographic techniques. Int J Pharm Sci Res 2012;3 Suppl 2:556-60.

Agrawal GR, Wakte P, Shelke S. Formulation, physicochemical characterization and in vitro evaluation of human insulin-loaded microspheres as a potential oral carrier. Prog Biomater 2017;6:125-36.

Kohli S, Sharma M, Pal A. Ethylcellulose floating microspheres of antidiabetic agent: in vitro and in vivo evaluation. Int J Appl Pharm 2017;9 Suppl 1:44-9.

Revathi S, Dhanaraju MD. Fabrication and effect of process variables of sitagliptin microspheres. Asian J Pharm Clin Res 2018;11 Suppl 4:291-7.

Navneet S, Madan P, Lin S. Effect of process and formulation variables on the preparation of parenteral paclitaxel-loaded biodegradable polymeric nanoparticles: a co-surfactant study. Asian J Pharm Sci 2016;11:404-16.

Vyslouzil J, Dolezel P, Kejdusova M, Maskova E, Masek J, Lukac R, et al. Influence of different formulations and process parameters during the preparation of drug-loaded PLGA microspheres evaluated by multivariate data analysis. Acta Pharm 2014;64:403-17.

Hoa LTM, Chi NT, Nguyen LH, Chien DM. Preparation and characterization of nanoparticles containing ketoprofen and acrylic polymers prepared by an emulsion solvent evaporation method. J Exp Nano Sci 2011;7 Suppl 2:189-97.




About this article

Title

DEVELOPMENT AND CHARACTERIZATION OF NOVEL HERBAL FORMULATION (POLYMERIC MICROSPHERES) OF SYZYGIUM CUMINI SEED EXTRACT

DOI

10.22159/ijap.2018v10i5.28624

Date

08-09-2018

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol 10, Issue 5 (Sep-Oct), 2018 Page: 226-234

Online ISSN

0975-7058

Authors & Affiliations

Ranu Biswas
Department of pharmaceutics, BCDA College of Pharmacy and Technology, Kolkata-700127, WB, India
India

Kalyan Kumar Sen
Department of pharmaceutics, Gupta College of Technological Sciences, Asansol-713301, WB, India


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