EFFICACY OF IONIC LIQUID [MIM]BR-BASED MAE ON RESVERATROL AND PHENOLIC COMPOUNDS EXTRACTION FROM GNETUM GNEMON SEEDS AND THEIR DPP-4 INHIBITORY ACTIVITY


Arif Arrahman, Rezi Riadhi Syahdi, Hana Permatasari, Intan Fikri Purnama Sari, Abdul MunÍm

Abstract


Objective: This study aimed to determine the inhibitory activity of 1-butyl-3-methylimidazolium bromide ([bmim]Br) extracts of melinjo seeds
(Gnetum gnemon) on dipeptidyl peptidase-4 (DPP-4).
Methods: Melinjo seeds were extracted by a [bmim]Br microwave-assisted method using various extraction parameters and the inhibitory activity
of DPP-4 of all extracts was determined in 96-well microplates using Cayman inhibitor screening assay. Determination of trans-resveratrol content
was conducted using a reverse-phase high-performance liquid chromatography method. The total phenolic content was determined using a 96-well
microplate reader. Data analysis for the determination of the optimal extraction conditions was developed by response surface methodology.
Results: The extract obtained from the third run showed the highest inhibition (28.73%) against DPP-4 activity with the total phenolic content of
1.96 mg gallic acid equivalent/g the seed powder.
Conclusion: The analytical results revealed the following optimal conditions: Solvent concentration 1.5 M, liquid-solid ratio 23:1, and extraction time
15 min.


Keywords


Gnetum gnemon, [bmim]Br, Trans-resveratrol, Microwave-assisted extraction, Dipeptidyl peptidase-4, Phenolic content.

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About this article

Title

EFFICACY OF IONIC LIQUID [MIM]BR-BASED MAE ON RESVERATROL AND PHENOLIC COMPOUNDS EXTRACTION FROM GNETUM GNEMON SEEDS AND THEIR DPP-4 INHIBITORY ACTIVITY

Keywords

Gnetum gnemon, [bmim]Br, Trans-resveratrol, Microwave-assisted extraction, Dipeptidyl peptidase-4, Phenolic content.

DOI

10.22159/ijap.2018.v10s1.36

Date

20-12-2018

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol. 10, Special Issue 1 (Dec.), 2018 Page: 168-171

Online ISSN

0975-7058

Authors & Affiliations

Arif Arrahman
Department of Pharmaceutical-Medicinal Chemistry and Bioanalysis, Faculty of Pharmacy, Universitas Indonesia, Depok 16424,

Rezi Riadhi Syahdi
Department of Pharmacognosy-Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia.

Hana Permatasari
Department of Pharmacognosy-Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia.

Intan Fikri Purnama Sari
Department of Pharmacognosy-Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia.

Abdul MunÍm
Department of Pharmacognosy-Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia.


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