A FORMULATION OF ORALLY DISINTEGRATING SECANG (CAESALPINIA SAPPAN L.) TABLETS AS AN ANTIOXIDANT WITH HYDROXYPROPYL CELLULOSE AS A MASKING AGENT

  • Resmi Mustarichie Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Indonesia
  • DRADJAD PRIAMBODO Department of Pharmaceutics (Apothecary Programme), Faculty of Pharmacy, Universitas Padjadjaran

Abstract

Objective: The objective of this research was to formulate Orally Disintegrating Tablets (ODT) preparations for secang (Caesalpinia sappan L.) wood extract as antioxidants that met the requirements as a pharmaceutical preparation.


Methods: Three ODT formulas were made using the composition of the extract of secang wood, HPC-H, kollidon® CL, Avicel® PH 101, mannitol, acesulfame, magnesium stearate, citric acid, and menthol. The bitter taste of the extract of wood secang was covered with the Hydroxy Propyl Cellulose High Substitution (HPC-H) masking agent. Variations in HPC-H concentration were 4%, 4.5%, and 5%. The evaluation of tablet print mass tested included water content, flow velocity, and resting angle, real density, incompressible density, and compressibility. Evaluation of tablets tested included weight uniformity, uniformity of size, hardness, friability, and disintegration time. The tablet antioxidant activity testing was carried out by the Diphenylpicylhydrazyl (DPPH) method.


Results: The results showed that all ODT formulas of secang wood extract were fulfilling the requirements as a tablet preparation according to Indonesian Pharmacopoeia IV. IC50 values ​​obtained from antioxidant testing on the three ODT formulas for a secang wood extract for formula I was 3.614 ppm, formula II was 3.464 ppm, formula III was 3.173 ppm, and the wood extract was 3.757 ppm.


Conclusion: The results obtained in this research work clearly indicated ODT formulas of secang wood extract fulfilled Indonesian Pharmacopea IV requirement and belong to the category of very strong antioxidants because they have an IC50 value of less than 50 ppm.

Keywords: Secang wood, Caesalpinia sappan, Antioxidant, Orally Disintegrating Tablets (ODT), DPPH

References

1. Singh RP, Sharad S, Kapur S. Free radicals and oxidative stress in neurodegenerative diseases: relevance of dietary antioxidants. JIACM 2004;5:218-25.
2. Scheibmeir HD, Christensen K, Whitaker SH, Jegaethesan J, Clancy R, Pierce JD. A review of free radicals and antioxidants for critical care nurses. Intensive Critical Care Nurs 2005;21:24-8.
3. Vardar Unlu G, Candan F, Sokmen A, Daferera D, Polissiou M, Sokmen M, et al. Antimicrobial and antioxidant activity of the essential oil and methanol extracts of Thymus pectinatus fisch. et mey. Var. pectinatus (Lamiaceae). J Agric Food Chem 2003;51:63-7.
4. Saefudin S, Pasaribu G, Sofnie S, Basri E. Effect of sappan wood (Caesalpinia sappan L) extract on blood glucose level in white rats. Indonesian J Forestry Res 2014;1:109–15.
5. Batubara I, Matsunaga T. Use of Indonesian medicinal plants against acne. Rev Agric Sci 2013;1:11-30.
6. Utari FD, Sumirat S, Djaeni M. Antioxidant production from secang wood extract (Caesalpinia sappan L.) using low humidity dryers. J Aplikasi Teknologi Pangan 2017;6:1-4.
7. Kumala S, Yuliani Y, Tulus D. The effect of giving steeping secang wood (Caesalpinia sappan L.) on mouse infected bacteria Escherichia coli. J Farmasi Indonesia 2009;4:188-98.
8. Hirani JJ, Rathod DA, Vadalia KR. Orally disintegrating tablets: a review. Trop J Pharm Res 2009;8:161-72.
9. Fauzi AM. Antioxidant activity of extracts and fractions of secang (Caesalpinia sappan L.) bark extract affected by temperature and pH using DPPH (Diphenylpicrylhydrazyl) method. Thesis. Bandung: Faculty of Pharmacy, Universitas Padjadjaran; 2012.
10. Badami S, Moorkoth S, Rai SR, Kannan E, Bhojraj S. Antioxidant activity of Caesalpinia sappan heartwood. Biol Pharm Bull 2003;26:1534-7.
11. Kusuma SAF, Mita SR, Firdayani I, Mustarichie R. Study on the antibacterial activity of fruit extracts of klutuk banana (Musa balbisiana Colla) against Shigella dysenteriae ATCC 13313. Asian J Pharm Clin Res 2017;10:220-3.
12. Mustarichie R, Ramdhani D, Iskandar Y. Characteristics and alopecia activity of pakis gajah (Angiopteris evecta (G. Forst) Hoffm.) growing in galunggung mountainside, West Java. Asian J Pharm Clin Res 2017;10:337-40.
13. Departemen Kesehatan RI. Indonesian pharmacopoeia. 4th ed. Jakarta: Departemen Kesehatan RI; 1995.
14. Liang AC, Chen LH. Fast-dissolving intraoral drug delivery systems. Exp Opinion 2001;11:981-6.
15. Nagar P, Singh K, Chauhan I, Verma M, Yasir M, Khan A, et al. Orally disintegrating tablets: formulation, preparation techniques, and evaluation. J Appl Pharm Sci 2011;1:35-45.
16. Badami S, Moorkoth S, Rai SR, Kannan E, Bhojraj S. Antioxidant activity of Caesalpinia sappan heartwood. Biol Pharm Bull 2003;26:1534-7.
17. Nirmal NP, Panichayupakaranant P. Antioxidant, antibacterial, and anti-inflammatory activities of standardized brazilin-rich Caesalpinia sappan extract. Pharm Biol 2014;53:1339-43.
18. Lim Y. Hedonic scaling: a review of methods and theory. Food Quality Preference 2011;22:733-47.
19. Chandra JH. Screening of antimicrobial activity and phytochemical analysis of Caesalpinia sappan L. J Chem Pharm Res 2013;5:171-5.
20. Kaur H, Amini MH, Prabhakar PK, Suttee A, Singh A. Phytochemical screening and antimicrobial activity of Caesalpinia sappan L. Leaves. Int J Pharm Pharm Res 2016;8:1064-9.
21. Srinivasan R, Selvam GG, Karthik S, Mathivanan K, Baskaran R, Karthikeyan M. In vitro antimicrobial activity of Caesalpinia sappan L. Asian Pacific J Trop Biomed 2012;2:S136-S139.
22. Departemen Kesehatan Republik Indonesia. Indonesian Herbal Pharmacopoeia (Indonesian: Farmakope Herbal Indonesia, Edisi I). Jakarta: Departemen Kesehatan Republik Indonesia; 2008.
23. Badan Standarisasi Nasional-BSN, SNI 7387-2009. Jakarta: Heavy Metal Pollution Requirements (in Indonesian); 2009.
24. Regulation of the Minister of Health of Indonesia (Indonesian: Peraturan Menteri Kesehatan Republik Indonesia); 2013.
25. General guidelines for methodologies on research and evaluation of traditional medicine. Geneva. World Health Organization, (WHO/EDM/TRM/2000.1); 2000.
26. Standar Nasional Indonesia (SNI). SNI 7387:2009 Maximum limit of heavy metal contamination in food (Indonesian: Batas Maksimum Cemaran Logam Berat Dalam Makanan). Jakarta: Badan Standardisasi Nasional; 2009.
27. NISSO HPC. High viscosity grade of hydroxypropyl cellulose (HPC-H) for hydrophilic matrix, sustained release formulation. Available from: www.nissoexcipients.com/PDF/Matrix_AppNote_CR2012AAPS.pdf [lAST accessed on 10 Dec 2018]
28. BASF. Kollidon® CL-F. Available from: https:// pharmaceutical.basf.com/en/Drug-Formulation/Kollidon-CL-F.html. [Last accessed on 14 Dec 2018].
29. Franc A, Kurhajec S, Pavlokova S, Sabadkova D, Muselik J. Influence of concentration and type of microcrystalline cellulose on the physical properties of tablets containing cornelian cherry fruits. Acta Pharm 2017;67:187–202.
30. Drugs.com. Mannitol. Available from: https://www.drugs.com/inactive/mannitol-142.html [Last accessed on10 Jan 2019]
31. Int'l Food Information Council Foundation. Everything You Need to Know About Acesulfame Potassium. Available from: https://www.nutritionexpress.com/article+index/vitamins+supplements+a-z/showarticle.aspx?id=120 [Last accessed on 05 Jan 2019].
32. Making Cosmetics. Magnesium Stearate, USP. Available from: https://www.makingcosmetics.com/Magnesium-Stearate-USP_p_297.html. [Last accessed on 30 Jan 2019]
33. Moncel B. Citric acid and how it's used in your food. Available from: https://www.thespruceeats.com/what-is-citric-acid-1328465 [Last accessed on 25 Jan 2019]
34. PubChem. Menthol. Available from: https://pubchem. ncbi.nlm.nih.gov/compound/menthol [Last accessed on 25 Jan 2019].
35. Aulton ME. Pharmaceutics: The Science of Dosage Form Design. Longman Group. Churchill Livingstone. New York, Part Four. Dosage Form Design and Manufacture; 2002.
36. Departemen Kesehatan Republik Indonesia. Indonesian Pharmacopoeia (Indonesian: Farmakope Indonesia), 3rd). Jakarta: Departemen Kesehatan Republik Indonesia; 1979.
37. FDA. Guidance for Industry: Orally Disintegrating Tablets; 2008. Available from: www.fda.gov/OHRMS/DOCKTES/ 98fr/FDA-2007-D-0365-gdl.pdf. [Last accessed on 15 Dec 2018].
38. Molyneux P. The use of stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol 2004;26:211-9.
39. Badami S, Moorkoth S, Rammanoharsingh S, Kannan E, Bhojraj S. Antioxidant activity of Caesalpinia sappan heartwood. Biol Pharm Bull 2003;26:1534-7.
40. Nirmal NP, Panichayupakaranant P. Antioxidant, antibacterial, and anti-inflammatory activities of standardized brazilin-rich Caesalpinia sappan extract. J Pharm Biol 2015;53:1339-43.
41. Kimestri AB, Indratiningsih I, Widodo W. Microbiological and physicochemical quality of pasteurized milk supplemented with sappan wood extract (Caesalpinia sappan L.), Int Food Res J 2018;25:392-8.
42. Noviza D, Suharti N, Lucida H, Pinto PB. Formulation of liposome using sappanwood (Caesalpinia Sappan L.) ethyl acetate fraction as an activating compound. Orient J Chem 2018;34:1611-8.
43. Febrianto N, Djumarti D, Rizki VM. Development of cardamom herbal coffee beverages: a study of physicochemical characteristics and consumer perception towards sensory properties. Pelita Perkebunan 2015;31:49-58.
44. Ong NE, Djarkasi GSS, Moningka JCS. Consumer level receipt level to sirsak (Annona muricata Linn) leaves tea drink Annona muricata linn (Indonesian: Tingkat Penerimaan Konsumen Terhadap Minuman Herbal Teh Daun Sirsak (Annona muricata Linn.), COCOS; 2016. p. 7.
45. Adinsi L, Akissoe NH, Dalod e-Vieira G, Anihouvi VB, Fliedel G, Mestres C, et al. Sensory evaluation and consumer acceptability of a beverage made from malted and fermented cereal: case ofgowe from Benin. Food Sci Nutr 2015;3:1-9.
Statistics
51 Views | 66 Downloads
Citatons
How to Cite
Mustarichie, R., & PRIAMBODO, D. (2019). A FORMULATION OF ORALLY DISINTEGRATING SECANG (CAESALPINIA SAPPAN L.) TABLETS AS AN ANTIOXIDANT WITH HYDROXYPROPYL CELLULOSE AS A MASKING AGENT. International Journal of Applied Pharmaceutics, 11(4), 236-241. https://doi.org/10.22159/ijap.2019v11i4.32663
Section
Original Article(s)