EFFECTIVITY OF 2.1% MINOCYCLINE OINTMENT AND 24% ETHYLENEDIAMINETETRAACETIC ACID GEL AS A ROOT SURFACE CONDITIONING MATERIAL (A STUDY OF FIBRIN CLOT ADHESION WITH SCANNING ELECTRON MICROSCOPE)

  • ADITYO WIDARYONO Department of Periodontics, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia.
  • YUNIARTI SOEROSO Department of Periodontics, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
  • SRI LELYATI Department of Periodontics, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia

Abstract

Objective: A stable fibrin clot adhesion on the root surface of the teeth is the start of periodontal regeneration. We aimed to observe the difference
between fibrin clot adhesion on root surfaces after root surface conditioning using minocycline and ethylenediaminetetraacetic acid (EDTA).
Methods: We divided 30 root surfaces into three groups – namely, the minocycline group, the EDTA group, and the saline group as a control. We
dropped fresh human blood on the root surface and allowed it to coagulate. We evaluated the fibrin clots using a scanning electron microscope.
Results: We found no significant differences between the minocycline and the EDTA applications (p=0.759).
Conclusions: EDTA and minocycline demonstrated equal capability in producing fibrin clots on the root surface.

Keywords: Periodontal regeneration, Root surface conditioning, Fibrin clot

References

1. Cobb CM. Non-surgical pocket therapy: Mechanical. Ann Periodontol
1996;1:443-90.
2. Drisko CH. Nonsurgical periodontal therapy. Periodontol
2000 2001;25:77-88.
3. Novak M, Novak K. Chronic periodontitis. In: Newman M, Takei H,
Klokkevold P, Carranza F, editors. Carranza’s Clinical Periodontology.
10th ed. St. Louis: Saunders; 2006. p. 494-9.
4. Pitaru S, Soldinger M, Madgar D, Metzger Z. Bacterial endotoxin
inhibits migration, attachment, and orientation of human gingival
fibroblasts in vitro and delays collagen gel contraction. J Dent Res
1987;66:1449-55.
5. Carranza F, Henry H, Cochran DL. Reconstructive periodontal surgery.
In: Newman M, Henry H, Klokkevold P, Carranza F, editors. Carranza’s
Clinical Periodontology. Philadelphia, PA: WB Saunders Co.; 2006.
p. 968-90.
6. Armitage G. Biologic Basis of Scaling and Root Planing: Biologic
Basis of Periodontal Maintenance Therapy. Berkeley: Praxis; 1980.
p. 78-115.
7. Polimeni G, Xiropaidis AV, Wikesjö UM. Biology and principles of
periodontal wound healing/regeneration. Periodontol 2000 2006;41:30-47.
8. Grzesik WJ, Narayanan AS. Cementum and periodontal wound healing
and regeneration. Crit Rev Oral Biol Med 2002;13:474-84.
9. Baker DL, Stanley Pavlow SA, Wikesjö UM. Fibrin clot adhesion
to dentin conditioned with protein constructs: An in vitro proof-ofprinciple
study. J Clin Periodontol 2005;32:561-6.
10. Polson AM, Frederick GT, Ladenheim S, Hanes PJ. The production of
a root surface smear layer by instrumentation and its removal by citric
acid. J Periodontol 1984;55:443-6.
11. Polson AM, Proye MP. Fibrin linkage: A precursor for new attachment.
J Periodontol 1983;54:141-7.
12. Hanes PJ, Polson AM, Ladenheim S. Cell and fiber attachment
to demineralized dentin from normal root surfaces. J Periodontol
1985;56:752-65.
13. Leite FR, Sampaio JE, Zandim DL, Dantas AA, Leite ER, Leite AA,
et al. Influence of root-surface conditioning with acid and chelating
agents on clot stabilization. Quintessence Int 2010;41:341-9.
14. Suchetha A, Darshan BM, Prasad R, Ashit GB. Root biomodification a
boon or bane? Indian J Oral Sci 2011;2:251-5.
15. Pitaru S, Melcher AH. Organization of an oriented fiber system in vitro
by human gingival fibroblasts attached to dental tissue: Relationship
between cells and mineralized and demineralized tissue. J Periodontal
Res 1987;22:6-13.
16. Heijl L, Sculean A. Application of enamel matrix proteins in intrabony
defect: A biology based regenerative treatment. In: Sculean A, editor.
Periodontal Regenerative Therapy. Berlin: Quintesscene; 2011. p. 90-102.
17. de Vasconcellos LM, Ricardo LH, Balducci I, de Vasconcellos LG,
Carvalho YR. Histological analysis of effects of 24% EDTA gel for
nonsurgical treatment of periodontal tissues. J Oral Sci 2006;48:207-14.
18. Wikesjo U, Polimeni G, Xiropaidis A, Stavropoulos A. Periodontal
wound healing/regeneration. In: Sculean A, editor. Periodontal
Regenerative Therapy. Berlin: Quintessence; 2011. p. 25-46.
19. Perno M. Pharmacotherapy in Periodontal Therapy. Access to Care
Position Paper. Access ADHA; 2001. p. 1-12.
20. Zhang H, Shang S, Li C. The use of Minocycline-Hcl Ointment for Root
Conditioning in Periodontal Surgery: A Preliminary Study. Hong Kong:
Paper Presented At: The 9th Asian Pacific Society of Periodontology
Meeting; 2011.
21. Greenwald RA, Golub LM, Lavietes B, Ramamurthy NS, Gruber B,
Laskin RS, et al. Tetracyclines inhibit human synovial collagenase
in vivo and in vitro. J Rheumatol 1987;14:28-32.
22. Atilla G, Baylas H. Effect of various demineralizing agents on mineral
contents of cementum surfaces (an electron probe analysis). J Marmara
Univ Dent Fac 1996;2:515-9.
23. Leite FR, Moreira CS, Theodoro LH, Sampaio JE. Blood cell attachment
to root surfaces treated with EDTA gel. Braz Oral Res 2005;19:88-92.
24. Minocha T, Rahul A. Comparison of fibrin clot adhesion to dentine
conditioned with citric acid, tetracycline, and ethylene diamine tetra
acetic acid: An in vitro scanning electron microscopic study. J Indian
Soc Periodontol 2012;16:333-41.
25. Oshel P. HMDS and specimen drying for SEM. Micros Today
1997;97:16.
26. Bansal P, Bansal P. Evaluation of fibrin clot adhesion as a precursor
for new attachment following root conditioning. Pak Oral Dent J
2010;30:176-83.
27. Blomlöf J, Lindskog S. Root surface texture and early cell and
tissue colonization after different etching modalities. Eur J Oral Sci
1995;103:17-24.
28. Theodoro LH, Sampaio JE, Haypek P, Bachmann L, Zezell DM,
Garcia VG, et al. Effect of Er: YAG and diode lasers on the adhesion of
blood components and on the morphology of irradiated root surfaces.
J Periodontal Res 2006;41:381-90.
29. Shetty B, Dinesh A, Seshan H. Comparitive effects of tetracyclines
and citric acid on dentin root surface of periodontally involved human
teeth: A scanning electron microscope study. J Indian Soc Periodontol
2008;12:8-15.
30. Somerman MJ, Foster RA, Vorsteg GM, Progebin K, Wynn RL. Effects
of minocycline on fibroblast attachment and spreading. J Periodontal
Res 1988;23:154-9.
31. Pitaru S, Gray A, Aubin JE, Melcher AH. The influence of the
morphological and chemical nature of dental surfaces on the migration,
attachment, and orientation of human gingival fibroblasts in vitro.
J Periodontal Res 1984;19:408-18.
32. Lee JY, Seol YJ, Park JR, Park YJ, Chung CP. Biological effects of
a root conditioning agent for dentin surface modification in vitro.
J Periodontal Implant Sci 2010;40:257-64.
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WIDARYONO, A., SOEROSO, Y., & LELYATI, S. (2019). EFFECTIVITY OF 2.1% MINOCYCLINE OINTMENT AND 24% ETHYLENEDIAMINETETRAACETIC ACID GEL AS A ROOT SURFACE CONDITIONING MATERIAL (A STUDY OF FIBRIN CLOT ADHESION WITH SCANNING ELECTRON MICROSCOPE). International Journal of Applied Pharmaceutics, 11(1), 59-63. https://doi.org/10.22159/ijap.2019.v11s1.166
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