PHYTOCHEMISTRY AND ANTIOXIDANT ACTIVITY OF SOURSOP (ANNONA MURICATA) LEAVES

  • FONA QORINA Medical Student, Faculty of Medicine, Universitas Indonesia,
  • ADE ARSIANTI Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia
  • QOTRUNNADA FITHROTUNNISA Medical Student, Faculty of Medicine, Universitas Indonesia
  • NADZILA ANINDYA TEJAPUTRI Medical Student, Faculty of Medicine, Universitas Indonesia

Abstract

Objective: Soursop (Annona muricata) is a tropical plant which has been utilized as a folk medicine to treat many diseases including cancer, inflammation and parasitic infection. In this study, we investigated its phytochemistry properties and antioxidant activity against free radicals.


Methods: Annona muricata leaves were extracted in three different solvents: ethanol, ethyl acetate and n-hexane. Afterwards, a phytochemistry test and the thin layer chromatography (TLC) method were used to evaluate bioactive compounds contained in the three different extracts. Antioxidant activity from the semi-polar (ethyl acetate) and polar (ethanol) solvents were evaluated by the DPPH (1,1-diphenyl-2-picrylhydrazyl) method, and the antioxidant activity is expressed by IC50 value. The results were compared to ascorbic acid as a positive control.


Results: The phytochemistry test showed that the extracts were positive for flavonoids, steroids, alkaloids, glycosides and tannins. Moreover, TLC analysis revealed that there were three chemical compounds contained in the extracts. The results from the DPPH method were that ethanolic extract was shown to have the most potent antioxidant activity with an IC50 value of 35.51 ppm.


Conclusion: The ethanolic extract of Annona muricata could be developed as the next promising natural antioxidant source.

Keywords: Antioxidants, DPPH method, Phytochemiscals, Soursop

References

1. Sies H, Jones D. Oxidative stress. In: Fink G. editor. Encyclopedia of stress. 2nd ed. San Diego: Elsevier; 2007. p. 45–8.
2. Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant defense. WAO J 2012;5:9–19.
3. Mahjoub S, Roudsari JM. Role of oxidative stress in pathogenesis of metabolic syndrome. Casp J Intern Med 2012;3:386–96.
4. Ichiishi E, Li XK, Iorio EL. Oxidative stress and diseases: clinical trials and approaches. Oxid Med Cell Longev 2016:1–3. Doi: 10.1155/2016/3458276
5. Muawanah M, Ahmad A, Natsir H. Antioxidant activity and toxicity polysaccharide extract from red algae Eucheuma cottonii and Eucheuma spinosum. Mar Chim Acta 2016;17:15–23.
6. Mohan S, Nandhakumar L. Role of various flavonoids: hypotheses on novel approach to treat diabetes. J Med Hypotheses Ideas 2014;8:1–6.
7. Istasse T, Jacquet N, Berchem T, Haubruge E, Nguyen BK, Richel A. Extraction of honey polyphenols: method development and evidence of cis isomerization. Anal Chem Insights 2016;16:49–57.
8. Quilez AM, Fernandez Arche MA, Garcia Gimenez MD, De la Puerta R. Potential therapeutic applications of the genus annona: local and traditional uses and pharmacology. J Ethnopharmacol 2018;225:244–70.
9. Pai BHM, Rajesh G, Shenoy R, Rao A. Anti-microbial efficacy of soursop leaf extract (Annona muricata) on oral pathogens: an in vitro study. J Clin Diagnostic Res 2016;10:ZC01–ZC04.
10. Astirin OP, Prayitno A, Artanti AN, Fitria MS, Witianingsih DA, Pranatami DA, et al. The expression of p53 and hsp70 proteins after treatment with Annona muricata linn leaf for activating apoptotic and lead to homeostasis program of raji cells technical report abstract. Int J Cancer Oncol 2014;2:1–6.
11. Olakunle S, Onyechi O, James O. Toxicity, anti-lipid peroxidation, in vitro and in vivo evaluation of antioxidant activity of Annona muricata ethanol stem bark extract. Am J Life Sci 2014;2:271–7.
12. Moghadamtousi SZ, Rouhollahi E, Karimian H, Fadaeinasab M, Firoozinia M, Abdulla MA, et al. The chemopotential effect of Annona muricata leaves against azoxymethane-induced colonic aberrant crypt foci in rats and the apoptotic effect of acetogenin annomuricin E in HT-29 cells: a bioassay-guided approach. PLoS One 2015;10:1–28.
13. George VC, Kumar DN, Suresh PK, Kumar RA. Antioxidant, DNA protective efficacy and HPLC analysis of Annona muricata (soursop) extracts. J Food Sci 2015;52:2328–35.
14. Barchan A, Bakkali M, Arakrak A, Pagan R, Laglaoui A. The effects of solvents polarity on the phenolic contents and antioxidant activity of three mentha species extracts. Int J Curr Microbiol Appl Sci 2014;3:399–412.
15. Radji M, Kurniati M, Kiranasari A. Comparative antimycobacterial activity of some Indonesian medicinal plants against multi-drug resistant mycobacterium tuberculosis. J Appl Pharm Sci 2015;5:19–22.
16. Coria Tellez AV, Montalvo Gonzalez E, Yahia EM, Obledo Vazquez EN. Annona muricata: a comprehensive review on its traditional medicinal uses, phytochemicals, pharmacological activities, mechanisms of action and toxicity. Arab J Chem 2018;11:662-91.
17. Gavamukulya Y, Wamunyokoli F, El-Shemy HA. Annona muricata: is the natural therapy to most disease conditions including cancer growing in our backyard? A systematic review of its research history and future prospects. Asian Pac J Trop Med 2017;10:835–48.
18. Moghadamtousi SZ, Fadaeinasab M, Nikzad S, Mohan G, Ali HM, Kadir HA. Annona muricata (Annonaceae): a review of its traditional uses, isolated acetogenins and biological activities. Int J Mol Sci 2015;16:15625–58.
19. Wulandari L, Lestyo. Kromatografi Lapis Tipis; 2016. p. 1–182.
20. Moon JK, Shibamoto T. Antioxidant assays for plant and food components. J Agric Food Chem 2009;57:1655–66.
21. Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol 2004;26:211-9.
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QORINA, F., ARSIANTI, A., FITHROTUNNISA, Q., & TEJAPUTRI, N. A. (2019). PHYTOCHEMISTRY AND ANTIOXIDANT ACTIVITY OF SOURSOP (ANNONA MURICATA) LEAVES. International Journal of Applied Pharmaceutics, 11(6). https://doi.org/10.22159/ijap.2019.v11s6.33524
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