POCKET MEASUREMENT METHODS IN WISTAR RATS PERIODONTITIS INDUCED BY BACTERIA AND THE INSTALLATION OF SILK LIGATURE: AN EXPERIMENTAL STUDIES

  • IGA AYU DHARMAWATI Post-Graduate, Faculty of Medicine, Udayana University, Bali, Indonesia,
  • I. B. PUTRA MANUABA Analitic Laboratory, Udayana University, Bali, Indonesia
  • HASANUDDIN THAHIR Departement of Periodontology, Faculty of Dentistry, Hasanuddin University, Makasar, Indonesia
  • I. MADE BAKTA Faculty of Medicine, Udayana University, Bali, Indonesia
  • I. N. MANTIK ASTAWA Faculty of Medicine, Udayana University, Bali, Indonesia
  • DEWA MADE SUKRAMA Faculty of Medicine, Udayana University, Bali, Indonesia
  • I. WYN PUTU SUTIRTA YASA Faculty of Medicine, Udayana University, Bali, Indonesia
  • TJOK GDE BAGUS MAHADEWA Faculty of Medicine, Udayana University, Bali, Indonesia
  • NI LUH KARTINI Faculty of Agrothecnology, Udayana University, Bali, Indonesia

Abstract

Objective: The purpose of this study was to develop an experimental model of induction of periodontal disease in Wistar rats, using a combination of bacterial induction and binding of silk ligature with respect to pocket periodontal depth.


Methods: Experiments with a pre-posttest group design was applied. Five adult male Wistar rats from the Udayana University's Analytical Laboratory were included in the study. Measuring pocket depth in experimental animals using dental probes was previously administered. Then performed the installation of silk ligature and bacterial induction Porphyromonas gingivalis, on the mandibular anterior teeth. Release of silk ligature on day 7, without the action of debridement of plaque or calculus on rat tooth. Observation of the development of the testing animals on the 3d, 7d, and 11d. On the 11d, re-measurement of pocket depth was conducted.


Results: Periodontal tissue abnormalities with silk ligature placement and bacterial infiltration Porphyromonas gingivalis cause periodontal inflammation with periodontal pocket formation, with a mean depth of 3.32 mm, which was analyzed using Wilcoxon p<0.05.


Conclusion: In this study combining bacterial induction and the installation of silk ligature can shorten the induction of periodontal tissue disease characterized by the formation of pocket periodontal.

Keywords: Wistar rat periodontitis, Periodontal pocket,, Periodontal probe

References

1. Djais AI, Tope VY. Effectiveness of siwak salvadora persica extract to aggregatibacter actinomycetemcomitans as one of the pathogenic bacteria causing periodontal disease. J Dentomaxillofac Sci 2017;2:28-31.
2. Moiden S, Khan RA, Siddiqui A. Efficacy of metronidazole in combination with amoxicillin doxycycline in adult periodontitis. J Basic Appl Sci 2013;9:99-104.
3. Ionel A, Lucaciu O, Moga M, Buhatel D, Hea A, Tabaran F, et al. Periodontal disease induced in wistar rats experimental study. Int J Bioflux Soc 2015;7:90-5.
4. Graves DT, Kang J, Andriankaja O, Wada K, Rossa C Jr. Animal models to study host bacteria interactions involved in periodontitis. Front Oral Biol 2012;15:117-32.
5. Struillou X, Boutigny H, Soueidan A, Layrolle P. Experimental animal models in periodontology: a review. Open Dent J 2010;4:37-47.
6. OZ Helieh S, Puleo DA. Animal models for periodontal disease. J Biomed Biotechnol 2011;8:75485.
7. Yustina AR, Suardita K, Agustin W. Increased number of osteoclasts in periapical inflammation caused by induction of lipopolysaccharide porphyromonas gingivalis (a study of laboratories using mice). JBP 2012;14:1.
8. Utama DBS, Arina YMD, Amin MN. Effect of peppaya leaf extract on the number of lymphocyte cells in gingiva of male wistar mice experiencing periodontitis. e-Jurnal Pustaka Kesehatan 2014;2:22-7.
9. Caranza FA, Newman MG, Takei HH, Klokkavoid PR. Caranza’s cinical periodontology. 11th ed. Elsevier Saunders: St Louis Missouri; 2012.
10. Siregar IHY, Supardan I, Sulistijarso N. The effect of paste of sukun leaves extract (artocarpus altilis) towards fibroblast cell and collagen tissues on periodontitis. Jurnal Riset Kesehatan 2015;4786-92.
11. Jacob SP, Nath S. Rat gingival model for testing drugs influencing inflammation. IeJSME 2013;7:8-16.
12. Kusumawardani B, Arina Y MD, Purwandhono A. Regulation of caspase-3 expression to maintain fetal growth in Porphyromonas gingivalis-infected pregnant rats. J Dentomaxillofac Sci 2016;1:1-6.
13. Solanki G. A general overview of periodontal pockets. Int J Biomed Res 2012;3:127-30.
14. Cekiki A, Kantarci A, Hasturk H, Dyke TE. Inflammatory and immune pathways in the pathogenesis of the periodontal disease. Periodontol 2014;64:57-80.
15. Hajishengallis G. Immunomicrobial pathogenesis of periodontitis: keystones, pathobionts, and host response. Trends Immunol 2014;35:3-11.
16. Maysak J, Podzimek S, Sommerova P, Mi YL, Bartova J, Janatova T, et al. Porphyromonas gingivalis: major periodontopathic pathogen overview. J Immunol Res 2014. Doi:10.1155/ 2014/476068.
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DHARMAWATI, I. A., MANUABA, I. B. P., THAHIR, H., BAKTA, I. M., ASTAWA, I. N. M., SUKRAMA, D. M., YASA, I. W. P. S., MAHADEWA, T. G. B., & KARTINI, N. L. (2019). POCKET MEASUREMENT METHODS IN WISTAR RATS PERIODONTITIS INDUCED BY BACTERIA AND THE INSTALLATION OF SILK LIGATURE: AN EXPERIMENTAL STUDIES. International Journal of Applied Pharmaceutics, 11(4), 71-74. https://doi.org/10.22159/ijap.2019.v11s4.35296
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