EFFECT OF LATEX FROM MUSA PARADISIACA VAR. SAPIENTUM AND CARICA PAPAYA L. ON PROLIFERATION AND MIGRATION FIBROBLAST CELL NIH3T3

  • ELZA SUNDHANI Cancer and Stem Cells Research Center, Universitas Muhammadiyah Purwokerto, Jl. Raya Dukuhwaluh PO. Box 202 Purwokerto 53182, Central Java, Indonesia, Department of Pharmacology and Clinical Pharmacy Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Jl. Raya Dukuhwaluh PO. Box 202 Purwokerto 53182, Central Java, Indonesia
  • ENDAH NUR IROHIM Cancer and Stem Cells Research Center, Universitas Muhammadiyah Purwokerto, Jl. Raya Dukuhwaluh PO. Box 202 Purwokerto 53182, Central Java, Indonesia. Department of Pharmacology and Clinical Pharmacy Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Jl. Raya Dukuhwaluh PO. Box 202 Purwokerto 53182, Central Java, Indonesia
  • RUMIYANA HARTININGSIH Cancer and Stem Cells Research Center, Universitas Muhammadiyah Purwokerto, Jl. Raya Dukuhwaluh PO. Box 202 Purwokerto 53182, Central Java, Indonesia. Department of Pharmacology and Clinical Pharmacy Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Jl. Raya Dukuhwaluh PO. Box 202 Purwokerto 53182, Central Java, Indonesia
  • ERZA GENATRIKA Department of Pharmacology and Clinical Pharmacy Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Jl. Raya Dukuhwaluh PO. Box 202 Purwokerto 53182, Central Java, Indonesia.
  • NUNUK ARIES NURULITA Cancer and Stem Cells Research Center, Universitas Muhammadiyah Purwokerto, Jl. Raya Dukuhwaluh PO. Box 202 Purwokerto 53182, Central Java, Indonesia. Department of Pharmacology and Clinical Pharmacy Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Jl. Raya Dukuhwaluh PO. Box 202 Purwokerto 53182, Central Java, Indonesia. Department of Pharmaceutical Chemistry Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Jl. Raya Dukuhwaluh PO. Box 202 Purwokerto 53182, Central Java, Indonesia.

Abstract

Objective: The objective of this research was to screen phytochemical constituents and determine the activity of latex from Musa paradisiaca
var. sapientum and Carica papaya L. to the process of wound healing in NIH3T3 fibroblasts cells through observations of the proliferation and
migration of cells.
Materials and Methods: Screening phytochemical compounds of latex from M. paradisiaca var. sapientum and C. papaya L. used chemical reagent.
Cytotoxic activity using 3-(4,5-dimethylthiazol-2-yl)-2.5-diphenyl tetrazolium bromide (MTT) method. The proliferation test used the doubling time
method at a susceptible incubation time of 0, 24, 48, and 72 h with a concentration of 25 μg/mL–250 μg/mL. The migration test was carried out using
the scratch wound healing method with a concentration of 25 μg/mL–250 μg/mL in the time range of 0, 12, 24, and 48 h.
Results: Phytochemical compounds contained in the latex from M. paradisiaca var. sapientum (saponin and tannin) and C. papaya L. (saponin and
alkaloid). The cytotoxic assay results showed that no toxic effect for NIH3T3 fibroblasts cells (IC50 >1000 μg/mL). Cell proliferation and migration
test results showed an increase in NIH3T3 fibroblast cell proliferation and migration process compared to controls. The concentration of 250 μg/mL
of latex from M. paradisiaca var. sapientum and C. papaya L. is the best to increase of proliferation and migration process of NIH3T3 fibroblast cells.
Conclusion: This study concludes that the latex of M. paradisiaca var. sapientum and C. papaya L. has the potential to increase proliferation and
migration activity of NIH3T3 cells.

Keywords: Musa paradisiaca var. sapientum, Carica papaya L., NIH3T3, Cell migration, Proliferation

References

1. Ajani RS, dan Ogunbiyi KI. Carica papaya latex accelerates wound
healing in diabetic Wistar rats. Eur J Med Plants 2015;9:1-12.
2. Anief M. Topical Drug Formulations by Skin Diseases. Yogyakarta:
UGM Press; 1997.
3. Fauzan F, Mardiyan KE, Widjiati W. Administering topical leaf extract
of papaya (Carica papaya L) on murine experimental animals (Mus
musculus) pregnant increases collagen density vaginal tissue. Majalah
Obstet Ginekol 2015;23:118-27.
4. Pongsipulung RG, Paulina YV, dan Yos B. The Formulation and
Testing of Pisang Ambon Extract Ointment (Musa paradisiaca var.
Sapientum (l.) Against the Open Sores on the Skin of a White Male
Wistar Strain Rats (Rattus norvegicus). Manado: Studi Farmasi FMIPA
UNSRAT. Farmasi POLTEKES; 2012.
5. Okoli RI, Aigbe O, Ohaju O, Mensah JK. Medicinal herbs used for
managing some common ailments among esan people of Edo state,
Nigeria. Pak J Nutr 2007;6:490-6.
6. Wijayanto. The Difference in Speed the Healing of Wounds With Sayat
Brand of Green Tea 6 Concentration, Povidone Iodine and 4gr% on
Mice. Malang: Universitas Muhammadiyah Malang; 2009.
7. Oky M, Manjas M, Putra AE, Agus S. Influence of fluid filtrate culture
fibroblasts (CFF) of wound healing; experimental research on strain
Wistar Rattus norvegicus. Andalas J Health 2012;1:1-13.
8. Kumar B, Vijayakumar M, Govindarajan R, Pushpangadan P.
Ethnopharmacological approaches to wound healing exploring
medicinal plants of India. J Ethnopharmacol 2007;114:103-13.
9. Suratman, Sumiwi SA, dan Gozali D. Influence of extracts of antana in
the form of ointments, ktim, and Jelly towards healing burns. Cermin
Dunia Kedokt 1996;108:31-6.
10. Robinson T. Kandungan Organik Tumbuhan Tinggi. Translated:
Padmawinata, K. Bandung: Penerbit ITB; 1995.
11. Padmasari PD, Astuti KW, dan Warditiani NK. Phytochemical screening
70% ethanol extract of the rhizome bangle (Zingiber purpureum Roxb.).
J Farmasi Udayana 2013;2:1-4.
12. Mahatriny NN, Payani NP, Oka IB, Astuti KW. Phytochemical
screening of ethanol extracts of leaves of Papaya (Carica papaya L.)
was obtained from the area of Ubud, Gianyar, Bali. J Farmasi Udayana
2014;13:11-2.
13. Cancer Chemoprevention Research Center. Cytotoxic Test of MTT
Method; 2013. Available from: http://www.ccrc.farmasi.ugm.ac.id.
[Last accessed on 2018 Jul 12].
14. Ira D. Cytotoxic test of 70% ethanol extract of herba cemplukan
(Physalis angulata Linn.) against WiDr cells. Journal Kimia Valensi
2008;1:149-56.
15. Mader SS. Human Biology. Lowa: McGraw Hill; 2000.
16. Kusumawardhani AD, Kalsum U, dan Rini IS. Effect of betel leaves
extracts ointment (Piper betle Linn.) on the number of fibroblast in IIA
degree burn wound on rat (Rattus norvegicus) Wistar strain. Majalah
Kesehatan FKUB 2015;2:16-28.
17. Listiyani AR. The Effect of the Giving of the Latex from
Musa paradisiaca var. Sapientum in the Process of Wound Healing in
Mice (Musculus albinus). Thesis. Bogor: Fakultas Kedokteran Hewan,
Institut Pertanian; 2006.
18. Priosoeryanto BP, Huminto H, Wientarsih I, Estuningsih S. Banana Tree
Stem Sap Activity in the Process of Wound Healing and Kosmetiknya
Effects on animals. Lembaga Penelitian dan Pemberdayaan Masyarakat.
Bogor: Institut Pertanian Bogor; 2006.
19. Gurung S, Skalko-Basnet N. Wound healing properties of carica
papaya latex: In vivo evaluation in mice burn model. J Ethnopharmacol
2009;121:338-41.
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SUNDHANI, E., IROHIM, E. N., HARTININGSIH, R., GENATRIKA, E., & NURULITA, N. A. (2019). EFFECT OF LATEX FROM MUSA PARADISIACA VAR. SAPIENTUM AND CARICA PAPAYA L. ON PROLIFERATION AND MIGRATION FIBROBLAST CELL NIH3T3. International Journal of Applied Pharmaceutics, 11(5), 31-35. https://doi.org/10.22159/ijap.2019.v11s5.T0035
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