ANTIBACTERIAL ACTIVITY OF CELERY LEAVES (APIUM GRAVEOLENS L.) FORMULATED IN TOOTHPASTE AGAINST STREPTOCOCCUS MUTANS
Objective: The objective of this research was to determine the antibacterial activity of the toothpaste from an extract of celery leaves on Streptococcus
Methods: The toothpaste was formulated with various concentrations of celery leaves, F1 with concentration of extract (6.25%), F2 (12.5%), and
F3 (25%). Each formula was tested the physical characteristics and antibacterial activity toward S. mutans. The antibacterial activity was determined
by the agar well diffusion method using brain heart infusion agar plates. Furthermore, the antibacterial activities were assessed by the presence or
absence of inhibition zones after the plates were incubated at 37°C for 24 h.
Results: The results from this test illustrate that all toothpastes under study at various concentrations of celery leaves extract exhibited antibacterial
activity. Maximum inhibition zone in antibacterial activity test was shown by F2 (12.5%). Therefore, we can use these toothpastes as natural
antibacterial on prevention of dental caries caused S. mutans.
Conclusion: The toothpaste from an extract of celery leaves showed significant antibacterial activity against S. mutans.
Ingredients and their Use. Oslo: Oslo University of Andidatus; 2003.
2. Silva DD, Gonçalo Cda S, Sousa Mda L, Wada RS. Aggregation of
plaque disclosing agent in a dentifrice. J Appl Oral Sci 2004;12:154-8.
3. Forssten SD, Björklund M, Ouwehand AC. Streptococcus mutans,
caries and simulation models. Nutrients 2010;2:290-8.
4. Nugraha AW. The Plaques Everywhere. Microbial Article. Yogyakarta:
Faculty of Pharmacy, USD; 2008. p. 1-3.
5. Perry DA, Beemsterboer P, Taggart EJ, Saunders WB. Periodontology
for the Dental Hygienist. St. Louis: Saunders Elsevier; 2007. p. 249-50.
6. Okpalugo J, dan Ibrahim K. Toothpaste formulation efficacy in reducing
oral flora. Trop J Pharm Res 2009;27:72.
7. Majidah D, Fatmawati DWA, Gunadi A. Antibacterial Power of Celery
Leaf Extract (Apium graveolens L) against the Growth of Streptococcus
mutans as an Alternative Mouthwash. New Jersey: Printice-HalI; 2014.
8. Nairn JG. Solutions, emulsions, suspensions and extracts. In:
Gennaro A, Marderosian AD, Hanson GR, Medwick T, Popovich NG,
Schnarre RL, et al. editors. Remington: The Science and Practice of
Pharmacy. 20th ed. Philadelphia, P.A: Lippincott Williams and Wilkins;
9. Irobi ON, Moo-Young M, Anderson WA, Daramola SO. Antimicrobial
activity of bark extracts of Bridelia ferruginea (Euphorbiaceae).
J Ethnopharmacol 1994;43:185-90.
10. Almawlah YH, Hadeel A, Al-Jelawi SO. Antibacterial activity of three
plant extracts against multidrug resistance pseudomonas aeruginosa.
Asian J Pharm Clin Res 2017;10:193-7.
11. Kamaliyah I. Comparison of the Effectiveness of Toothpaste Extract
Formulations of Red Betel Leaves (Piper crocatum Ruiz and Pav)
Between PGA, PEG and HPLC Bases Against Streptococcus mutans
and Lactobacilllus [Thesis]. Faculty of Pharmacy, Universitas
Muhammadiyah Purwokerto; 2015.
12. Nursal FK, Indriani O, Dewantini LA. Use of Na CMC as a Gelling
agent in Toothpaste Formulations of 70% Ethanol Extract of Guava
Leaves (Psidium guajava L). Jakarta: Department of Pharmacy,
13. Sujono TA, Wahyuni US. Effect of Aloe vera leave decocta
(Aloe vera L) against the blood glucose levels of the rabbits who are
burdened glucose. J Sci Technol Res 2005;6;26-34.
14. Panda S, Bandyopadhyay PK. Chemical information from GC-MS
studies of methanolic leaf extract of Andrographis paniculata and Datura
metel and their antibacterial activity against isolated pseudomonas
aeruginosa (pb112) strain. Int J Pharm Bio Sci 2013;4:909-15.
15. Nwaogu LA, Alisi CS, Ibegbulem CO, Igwe CU. Phytochemical and
antimicrobial activity of ethanolic extract of Landolphia owariensis
leaf. Afr J Biotechnol 2007;6:890-93.
16. Iwu MW, Duncan AR, Okunji CO. New antimicrobials of plant
origin. In: Janick J editor. Perspectives on New Crops and New uses.
Alexandria: ASHS Press; 1999. p. 457-62.
17. Cushnie TP, Lamb AJ. Antimicrobial activity of flavonoids. Int J
Antimicrobial Agents 2005;26:343-56.
18. Al-Bayati FA, Al-Mola HF. Antibacterial and antifungal activities of
different parts of Tribulus terrestris L. Growing in Iraq. J Zhejiang Univ
Sci B 2008;9:154-9.
19. Arabski M, Wegierek-Ciuk A, Czerwonka G, Lankoff A, Kaca W.
Effects of saponins against clinical E. coli strains and Eukaryotic cell
line. J Biomed Biotechnol 2011;2012:6.
20. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol
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