EVALUATION OF MYRICETIN NANOPHYTOSOME WITH THIN-SONICATION LAYER HYDRATION METHOD USING ETHANOL AND ACETONE SOLVENTS
Keywords:Nano-phytosome, Myricetin, Thin layer hydration, Sonication
Objective: Nano-phytosome is a nanotechnology that is used to improve the bioavailability of active ingredients contained in plants by binding to active ingredients with phospholipids which have properties that resemble cell membranes. The active ingredient used in the nano-phytosome formulation is myricetin. Myricetin is a natural flavonoid compound that has antioxidant properties with low bioavailability and permeability. The purpose of this study was to determine the characterization of the nano-phytosome myricetin formulation with different solvents using ethanol and acetone.
Methods: Nano-phytosome was made using a thin-sonication hydration method by comparing the acetone and ethanol solvents as well as the variation of the myricetin: phosphatidylcholine: cholesterol ratio. Characterization of nano-phytosome includes particle size, polydisperse index, zeta potential, absorption efficiency and antioxidant activity, and TEM test.
Results: Characterization and evaluation of myricetin nano-phytosome using two different solvents, acetone and ethanol. The particle size of all formulas has a size between 10-1000 nm, the use of ethanol solvent produces the smallest particle size of 198.1 ± 1,74 nm and the lowest polydispersity index of 0.175 ± 0,020 in ethanol solvent.
Conclusion: The ethanol solvent is better compared to the acetone solvent, and the best formula is formula 4 with the ratio of myricetin: phosphatidylcholine: cholesterol (1: 1: 0.4).
Mahajan T, MC. A novel approach towards phytosomal flavonoids. Pharma Sci 2012;4:2079-121.
Guardia T, Rotelli AE, Juarez AO, Pelzer LE. Antiinflammatory properties of plant flavonoids. Effects of rutin, quercetin and hesperidin on adjuvant arthritis in rat. Il Farmaco 2001;56:683-37.
Wang L, Wang B, Li H, Lu H, Qiu F, Xiong L, et al. Quercetin, a flavonoid with anti-inflammatory activity, suppresses the development of abdominal aortic aneurysms in mice. Eur J Pharmacol 2012;690:133-41.
Hong C. Effect of stabilizing agents on the development of myricetin nanosuspension and its characterization: an in vitro and in vivo evaluation. Int J Pharm 2014;477:251-60.
Papakostas D, Rancan F, Sterry W, Peytavi UB, Vogt A. Review article: nanoparticles in dermatology. arch dermatol res. Springer Verlag Berlin Germany 2011;303:533–50.
Rasaie S. Nano phytosomes of quercetin: a promising formulation for the fortification of food products with antioxidants. Pharma Sci 2014;20:96-101.
Ramadon, Munim A. Utilization of nanotechnology in new drug delivery systems for natural material products. Indonesian J Pharm 2016;14:118-27.
Jain N, Gupta BP, Thakur N, Jain R, Banweer J, Jain D, et al. Phytosome: a novel drug delivery system for herbal medicine. Int J Pharm Sci Drug Res 2010;2:224‐8.
Junghanns AH, dan Müller RH. Nanocrystal technology, drug delivery and clinical applications. Int J Nanomed 2008;3:295-309.
Gupta RB, dan Kompella UB. Fundamentals of drug nanoparticles, naoparticles technology for drug delivery. Taylor Franscis New York 2006;13:1-20.
Ghanbarzadeh B. Nano-phytosome as a potential food-grade delivery system. Food Biosci 2016;15:126-35.
Freag MS. Lyophilized phytosomal nanocarriers as platforms for enhanced diosmin delivery: optimization and ex vivo permeation. Int J Nanomed 2013;8:2385-97.
Mehanna M, Elmaradny H, Samaha M. Mucoadhesive liposomes as ocular delivery system: physical, microbiological, and in vivo assessment. Drug Dev Ind Pharm 2010;36:108-18.
Meenakshi K, Parvat K, Ashwani S, Divya D, Mayank K, Nidhi S. Formulation, characterization and in vitro evaluation of tactically engineered proniosomes for successful oral delivery of ramipril. Pharm Lett 2015;7:93-7.
Yang T, Cui FD, Choi MK, Lin H, Chung SJ, Shim CK, et al. Liposome formulation of paclitaxel with enhanced solubility and stability. Drug Delivery 2007;14:301-8.
Kedare SB, Singh RP. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol 2011;48:412–22.
Nasr A, Gardouh A, Ghonaim H, Abdelghany E, Ghorab M. Effect of oils, surfactants and cosurfactant on phase behavior and physicochemical properties of self-nano emulsifying drug delivery system (SNEDDS) for irbesartan and olmesartan. Int J Appl Pharm 2016;8:13-24.
Mohanraj VJ, Chen Y. Nanoparticles-a review. J Pharm Res 2006;5:561-73.
Jonassen H. Polysaccharide based nanoparticle for drug delivery application [Thesis School of Pharmacy]. Faculty of Matematics and Natural Science, University of Oslo; 2014.
Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin. J Sci Technol 2004;26:211-9.
Redha A. Flavonoid: structure, antioxidative properties and their role in biological systems. J Belian 2010;9:196-202.