TOPICAL ANTI-PSORIATIC NANOPARTICULATE DRUG DELIVERY SYSTEM

  • KIRAN BHISE Department of Pharmaceutics, M. C. E. Society’s Allana College of Pharmacy, Pune, India
  • SHADAB KHAN Department of Pharmaceutics, M. C. E. Society’s Allana College of Pharmacy, Pune, India
  • GHAZALA MULLA Department of Physiology, Z. V. M. Unani Medical College and Hospital, Pune, India

Abstract

Objective: Development of effective drug delivery in the treatment of psoriasis is the major challenge for its successful management. To develop and assess the potential of Nanostructured Lipid Carriers (NLCs) enriched with the powdered leaves extracts of Azadirachta indica (AE), Lawsonia inermis (LE) and fruit extract of Mallotus philippensis (ME) in the management of psoriasis.


Methods: Drug loaded NLCs were prepared via hot homogenization technique by adopting 23 factorial design with factors X1 as the concentration of lipids, X2 concentration of surfactants and X3 being the number of homogenization cycle. The responses Y1 and Y2 were particle size and zeta potential. The optimized batch was obtained from Surface response plot and was evaluated for zeta potential, % entrapment efficiency, % drug loading, Scanning Electron Microscopy(SEM), % in vitro diffusion of drugs from the NLCs, anti-lipid peroxidation and nitric oxide scavenging activities, cytotoxicity on HaCat cell lines, Mouse Tail and Rat ultraviolet ray B photodermatitis models for Psoriasis.


Results: The optimized batch of NLCs was found within the nanosized range with a relatively low polydispersity index and zeta potential of-20mV. The %EE for an optimized batch of NLCs was found to be 98.97±0.83%, 96.99±0.56% and 99.25±0.55% and the %DL of 21.84±0.15%, 8.55±045%, and 87.91±0.38% respectively for AE, LE and ME.


The SEM images showed the spherical vesicular structures of drugs loaded NLCs. The in-vitro diffusion of drugs from the NLCs followed initial burst release thereafter sustained release for 24 h. The AE, LE and ME loaded NLCs proved to possess anti-lipid peroxidation and nitric oxide scavenging activities, cytotoxicity on HaCat cell lines, DNA fragmentation on HaCat cell lines which are biomarkers in the pathogenesis of psoriasis. The results of Mouse Tail and Rat ultraviolet ray B photodermatitis models for Psoriasis supported the anti-psoriatic potential of AE, LE and ME loaded NLCs.


Conclusion: AE, LE and ME loaded NLCs can be used for prolonged topical delivery to the psoriatic skin for an effective treatment.

Keywords: Psoriasis, Drug Extracts, Nanostructured Lipid Carriers, Evaluation

References

1. Y Madhusudan Rao, Shayeda. Cosmeceuticals. PharmaMed Press; 2012. p. 194-253.
2. Magina S, Julia Vide. Moderate to severe psoriasis treatment challenges through the era of biological drugs. An Bras Dermatol 2017;92:668-74.
3. Chandrasekar R, Sivagami B. Alternative treatment for psoriasis-a review. Int J Res Dev Pharm L Sci 2016;5:2188–97.
4. Lowes MA, AM Bowcock, JG Krueger. Pathogenesis and therapy of psoriasis. Nature 2007;445:866-73.
5. Patricia M. Witman, topical therapies for localized psoriasis. Myo Clin Proc 2001;76:943-9.
6. Raza K, Singh B, Dogra S. Novel drug delivery systems in the topical treatment of psoriasis: rigors and vigors. Indian J Dermatol Venereol Leprol 2010;76:612-21.
7. Agarwal R, Katare OP, Vyas SP. Preparation and in vitro evaluation of liposomal/niosomal delivery systems for antipsoriatic drug dithranol. Int J Pharm 2001;228:43-52.
8. Ali MF, Salah M, Rafea M, Saleh N. Liposomal methotrexate hydrogel for the treatment of localized psoriasis: preparation, characterization and laser targeting. Med Sci Monit 2008;14:66-74.
9. Abdelbary AA, Abou Ghaly MH. Design and optimization of topical methotrexate loaded niosomes for enhanced management of psoriasis: Application of Box-Behnken design, in vitro evaluation, and in vivo skin deposition study. Int J Pharm 2015;485:235-43.
10. Trapasso E, Cosco D, Celia C, Fresta M, Paolino D. Retinoids: new use by innovative drug-delivery systems. Expert Opin Drug Delivery 2009;6:465-83.
11. Umalkar DG, Rajesh KS. Formulation and evaluation of liposomal gel for the treatment of psoriasis. Int J Pharma Bio Sci 2013;4:22-32.
12. Purushothamrao K, Khaliq K, Sagare P, Patil SK, Kharat SS, Alpana K. Formulation and evaluation of vanishing cream for scalp psoriasis. Int J PharmaSciTech 2010;4:32-31.
13. Su YH, Fang JY. Drug delivery and formulations for the topical treatment of psoriasis. Expert Opin Drug Delivery 2008;5:235-49.
14. Pradhan M, Singh D, Singh MR. Novel colloidal carriers for psoriasis: Current issues, mechanistic insight, and novel delivery approaches. J Controlled Release 2013;170:380-95.
15. Suresh PK, Singh P, Saraf S. Novel topical drug carriers as a tool for the treatment of psoriasis: progress and advances. Afr J Pharm Pharmacol 2013;7:138-47.
16. Dubey V, Mishra D, Dutta T, Nahar M, Saraf DK, Jain NK. Dermal and transdermal delivery of an anti-psoriatic agent via ethanolic liposomes. J Controlled Release 2007;123:148-54.
17. Zhang YT, Shen LN, Wu ZH, Zhao JH, Feng NP. Comparison of ethosomes and liposomes for skin delivery of psoralen for psoriasis therapy. Int J Pharm 2014;471:449-52.
18. Sah AK, Jain SK, Pandey RS. Microemulsion based hydrogel formulation of methoxsalen for the effective treatment of psoriasis. Asian J Pharm Clin Res 2011;4:140-5.
19. Behera J, Keservani RK, Yadav A, Tripathi M, Chadoker A. Methoxsalen loaded chitosan coated microemulsion for effective treatment of psoriasis. Int J Drug Delivery 2010; 2:159-67.
20. Lin YK, Huang ZR, Zhuo RZ, Fang JY. The combination of calcipotriol and methotrexate in nanostructured lipid carriers for topical delivery. Int J Nanomed 2010;5:117-28.
21. Lapteva M, Mondon K, Möller M, Gurny R, Kalia YN. Polymeric micelle nanocarriers for the cutaneous delivery of tacrolimus: a targeted approach for the treatment of psoriasis. Mol Pharm 2014;11:2989-3001.
22. Fang JY, Fang CL, Liu CH, Su YH. Lipid nanoparticles as vehicles for topical psoralen delivery: solid lipid nanoparticles (SLN) versus nanostructured lipid carriers (NLC). Eur J Pharm Biopharm 2008;70:633-40.
23. Pople PV, Singh KK. Development and evaluation of colloidal modified nano lipid carrier: application to topical delivery of tacrolimus, Part II–in vivo assessment, drug targeting, efficacy, and safety in treatment for atopic dermatitis. Eur J Pharm Biopharm 2013;84:72-83.
24. Pinto MF, Moura CC, Nunes C, Segundo MA, Costa Lima SA, Reis S. A new topical formulation for psoriasis: development of methotrexate-loaded nanostructured lipid carriers. Int J Pharm 2014;477:519-26.
25. Raza K, Singh B, Lohan S, Sharma G, Negi P, Yachha Y. Nano-lipoidal carriers of tretinoin with enhanced percutaneous absorption, photostability, biocompatibility, and anti-psoriatic activity. Int J Pharm 2013;456:65-72.
26. Surya Tej KVM, Moin A, Gowda DV, Anjali, Karunakar G, Patel NP. Nanostructured lipid carrier-based drug delivery system. J Chem Pharm Res 2016;8:627–43.
27. Gungor S, Rezigue M. Nanocarriers mediated topical drug delivery for psoriasis treatment. Curr Drug Metab 2017;18:454-68.
28. Gazi Shaikh, Sadath Ali, SY Talmale, Ulhas S Surwase, Kadam Bhalchandra, Shaikh Luqman. Alternative medicine for psoriasis–natural herbal ayurvedic treatment-a review. Int J Ayurvedic Herbal Med 2012;2:455–63.
29. Kaur T. Formulation development of anti-psoriatic topical babchi oil emulgel formulation development of anti-psoriatic topical babchi oil emulgel. Res Rev: J Herbal Sci 2017;2:1-14.
30. Chandrasekar R, Sivagami B. Alternative treatment for psoriasis-a review. Int J Res Dev Pharm Life Sci 2016;5:2188–97.
31. Naik MR, Bhattacharya A, Behera R, Agrawal D, Dehury S, Kumar S. Study of the anti-inflammatory effect of Azadirachta indica seed oil (Azadirachta indica) on infected albino rats. J Herbal Res Rev 2014;1:66–9.
32. Wiem A, Smail A, Wissem M, Faleiro M, Miguel M. Antioxidant, anti-inflammatory and anti-acetylcholinesterase activities of leaf, flower, and seed aqueous extracts of lawsonia inermis from tunisia. Int J Pharm Pharm Sci 2014;6:445–52.
33. Annavarapu T Rao, Renuka P, Akhil P, Divya P, P DP. Evaluation of the anti-inflammatory activity of combination of ethanol extracts of azadirachta Indica (Azadirachta indica) and lawsonia inermis (Lawsonia inermis). Asian J Pharm Clin Res 2016;9:9–11.
34. Gangwar M, Gautam MK, Ghildiyal S, Nath G, Goel RK. Pharmacological evaluation of mallotus philippinensis (Lam.) muell. Arg. fruit hair extract for anti-inflammatory, analgesic and hypnotic activity. J Intercult Ethnopharmacol 2016;5:14–21.
35. Ghazanavi, Khaklid. Amraz-e-Jildaur Ilaj-e-Nabvi. Farid Book Pvt Ltd; 2004. p. 123-136, 214-217, 257, 276.
36. Jayakumar Jerobin, Pooja Makwana, RS Suresh Kumar, Rajiv Sundaramoorthy, Amitava Mukherjee, Natarajan Chandrasekaran. Antibacterial activity of neem nanoemulsion and its toxicity assessment on human lymphocytes in vitro. Int J Nanomed 2015;10:77-86.
37. V Vijayan, Shaik Aafreen, S Sakthivel, K Ravindra Reddy. Formulation and characterization of solid lipid nanoparticles loaded Neem oil for topical treatment of acne. J Acute Disease 2013;2:282-6.
38. Tatiane Pasquoto Stigliani, Estefânia VR Campos, Jhones L Oliveira, Camila MG Silva, Natalia Bilesky Jose, Mariana Guilger, et al. Nanocapsules containing neem (Azadirachta Indica) oil: development, characterization, and toxicity evaluation. Sci Reports 2017;7:1-12.
39. SCG Kiruba Daniel, N Mahalakshmib, J Sandhiya, Kasi Nehru, Muthusamy Sivakumar. Rapid synthesis of Ag nanoparticles using henna extract for the fabrication of photoabsorption enhanced dye-sensitized solar cell (PEDSSC). Adv Materials Res 2013;678:349-60.
40. Mahmood Barani, Mohammad Mirzaei, Masoud Torkzadeh Mahani, Mohammad Hadi Nematollahi. Lawsone-loaded niosome and its antitumor activity in MCF-7 breast cancer cell line: a nano-herbal treatment for cancer. DARU J Pharm Sci 2018;26:11–7.
41. Qiang Xia, Hongxia Wang. Preparation and characterization of co-enzyme Q10 loaded nanostructured lipid carriers as delivery system for cosmetic component. NSTI-Nanotech 2010;3:498-501.
42. Banker GS, Rhodes CT. Modern pharmaceutics. 3rd edition. Optimization techniques in pharmaceutical formulation and processing. Marcel Dekker; 1996. p. 607.
43. Uprit S, Kumar Sahu R, Roy A, Pare A. Preparation and characterization of minoxidil loaded nanostructured lipid carrier gel for effective treatment of alopecia. Saudi Pharm J 2013;21:379–85.
44. Fouad EA, Yassin AEB, Alajami HN. Characterization of celecoxib-loaded solid lipid nanoparticles formulated with tristearin and softisan 100. Trop J Pharm Res 2015;14:205–10.
45. Souto EB, Wissing SA, Barbosa CM, Müller RH. Development of a controlled release formulation based on SLN and NLC for topical clotrimazole delivery. Int J Pharm 2004;278:71–7.
46. Chander Prakash Dora, Shailendra Kumar Singh, Sanjeev Kumar. Development and characterization of nanoparticles of glibenclamide by solvent displacement method. Acta Poloniae Pharm Drug Res 2010;67:283-90.
47. Gaba B, Fazil M, Khan S, Ali A, Baboota S, Ali J. Nanostructured lipid carrier system for topical delivery of terbinafine hydrochloride. Bull Fac Pharm Cairo Univ 2015;53:147–59.
48. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95:351-8.
49. Panasenko OM, Evgina SA, Driomina ES. Hypochlorite induces lipid peroxidation in blood lipoproteins and phospholipid liposomes. Free Radical Biol Med 1995;19:133-40.
50. Yokozawa T, Wang ST, Chen PC, Hattori M. Inhibition of nitric oxide release by an aqueous extract of tinospora tuberculata. Phytother Res 2000;14:51-3.
51. T Mosmann. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:12-6.
52. Colombo I, Sangiovanni E, Maggio R, Mattozzi C, Zava S, Corbett Y, et al. HaCaT cells as a reliable in vitro differentiation model to dissect the inflammatory/repair response of human keratinocytes. Medistors of Inflammation 2017:1–12. Doi:10.1155/ 2017/ 7435621.
53. Li Z, Yu J, Liu L, Wei Z, Ehrlich ES. Coxsackie virus A16 infection induces neural cell and non-neural cell apoptosis in vitro. PLOS One 2014;9:1-10.
54. Takada M, Noguchi A, Sayama Y, Kurohane Kaneko Y, Ishikawa T. Inositol 1,4,5-trisphosphate receptor-mediated initial Ca(2+) mobilization constitutes a triggering signal for hydrogen peroxide-induced apoptosis in INS-1 ?-cells. Biol Pharm Bull 2011;34:954-8.
55. Singhal M, Kansara N. Cassia tora linn cream inhibits psoriasis in mouse tail model. Pharm Crops 2012;3:1-6.
56. Manmohan Singhaland Niraj Kansara. Cassia tora linn cream inhibits ultraviolet-b-induced psoriasis in rats. ISRN Dermatol 2012;16:6.
57. Nakaguma H, Kambara T, Yamamoto T. Rat ultraviolet ray B photodermatitis: an experimental model of psoriasis vulgaris. Int J Exp Pathol 1995;76:65–73.
58. Nagamani M, Prahaladu P, Vijayababu PVSS, Ashalata K, Kusuma Kumari P, Kumari KL. Lipid peroxidation product as a marker of oxidative stress in psoriasis-a case-control study in north coastal Andhra Pradesh. IOSR J Dent Med Sci Ver II 2015;14:2279–861.
59. Ignacio NRS, Ferreira PLJ, Almeida BM, Kubelka FC. Nitric oxide production by murine peritoneal macrophages in vitro and in vivo treated with Phyllanthus tenellus extracts. J Ethnopharmacol 2001;74:181-7.
60. Shantanu Kuchekar, Kiran Bhise. Formulation and development of antipsoriatic herbal gel-cream. J Sci Industrial Res 2012;71:279-84.
61. Rajan A. Case study on beta-blockers induced psoriasis. Int J Pharm Pharm Sci 2019;11:112-5.
62. Pai G, N Sashidharan. Biologic armamentarium in psoriasis. Asian J Pharm Clin Res 2006;9:65-72.
Statistics
118 Views | 118 Downloads
Citatons
How to Cite
BHISE, K., KHAN, S., & MULLA, G. (2020). TOPICAL ANTI-PSORIATIC NANOPARTICULATE DRUG DELIVERY SYSTEM. International Journal of Applied Pharmaceutics, 12(2), 76-85. https://doi.org/10.22159/ijap.2020v12i2.36536
Section
Original Article(s)