• SHADAB KHAN Department of Pharmaceutics, M. C. E. Society’s Allana College of Pharmacy, Pune, India
  • GHAZALA MULLA Department of Physiology, Z. V. M. Unani Medical College and Hospital, Pune, India
  • KIRAN BHISE Department of Pharmaceutics, M. C. E. Society’s Allana College of Pharmacy, Pune, India



Herbal Anti-psoriatic Nanoparticulate Cream, Cell line study, Advanced Preclinical Trials


Objective: The lack of possible cure and associated disadvantages of allopathic medicines in the topical treatment of psoriasis has led to extensive research for anti-psoriatic activities of plant-based drugs. The present study was aimed to develop topical cream formulation containing the Nanostructured Lipid Carriers of Azadirachta indica leaves extract (AE), Lawsonia inermis leaves extract (LE) and fruit extract of Mallotus philippensis (ME) and assessing the antipsoriatic activity of prepared cream formulation.

Methods: The Drug loaded NLCs were prepared via hot homogenization technique and incorporated into the water in oil (w/o) cream base that was prepared by the emulsification method. Evaluation of cream formulation included advanced preclinical trials using Rat Ultraviolet Ray-B photodermatitis and Mousetail method, anti-lipid peroxidation assay, nitric oxide scavenging activity, cytotoxicity and DNA fragmentation on HaCat cell lines to ascertain antipsoriatic potential and in vitro drug diffusion.

Results: In spite of the low amount of the drug loading in NLCs their potency was much higher as displayed in Advanced Preclinical studies and cell line studies for psoriasis indicating the suitability of NLCs loaded creams for skin applications due to their various desirable effects on the skin. Occlusive properties of the prepared NLC on the skin provide an increase in drug penetration particularly via skin moisturization. The in vitro drug diffusion studies suggest the prolonged and almost complete release of AE, LE and ME from NLC based cream up to 24 h that was found to be 81.24±0.51%, 74.31±0.25% and 70.43±0.78% respectively. The literature survey supports the novelty of such topical anti-psoriatic polyherbal nanoparticulate cream.

Conclusion: It can be concluded that the AE, LE and ME loaded NLC based cream can be used for prolonged topical delivery of drugs for the management of Psoriasis.


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Raghupatruni Jhansi Laxmi, R Karthikeyan, P Srinivasa Babu, RVV Narendra Babu. Formulation and evaluation of antipsoriatic gel using natural excipients. J Acute Disease 2013;2:115-21.

Tejinder Kaur Marwaha. Formulation development of anti-psoriatic topical babchi oil emulgel. Res Rev: J Herbal Sci 2013;2:1-10.

Gazi Shaikh, Sadath Ali, SY Talmale, Ulhas S Surwase, Kadam Bhalchandra, Shaikh Luqman. Alternative medicine for psoriasis–a natural herbal ayurvedic treatment-a review. Int J Ayurvedic Herbal Med 2012;2:455–63.

Kaur T. Formulation development of anti-psoriatic topical babchi oil emulgel formulation development of anti-psoriatic topical babchi oil emulgel. Res Rev: J Herbal Sci 2017;2:1-14.

Chandrasekar R, Sivagami B. Alternative treatment for psoriasis-a review. Int J Res Dev Pharm Life Sci 2016;5:2188–97.

Ghazanavi, Khaklid. Amraz-e-Jildaur Ilaj-e-Nabvi. Farid Book Pvt Ltd; 2004. p. 123-136, 214-7, 257, 276.

Shashank Kumar, Pandey AK. Chemistry and biological activities of flavonoids: an overview. Sci World J 2013:1-16. Doi:10.1155/2013/162750

R Chaibi, M Romdhane, A Ferchichi, J Bouajila. Assessment of antioxidant, anti-inflammatory, anti-cholinesterase and cytotoxic activities of Henna (Lawsonia inermis) flowers. J Natural Products 2015;8:85-92.

Mayank Gangwar, RK Goel, Gopal Nath. Mallotus philippinensis muell. Arg (Euphorbiaceae): ethnopharmacology and phytochemistry review. BioMed Res Int 2014:1-13.

Li B, Ge ZQ. Nanostructured lipid carriers improve skin permeation and chemical stability of idebenone. AAPS PharmSciTech 2012;13:276-83.

Leelapornpisid P, Chansakaow S, Na-boon long S, Jantrawut P. Development of cream containing nanostructured lipid carriers loaded marigold flowers extract for anti-wrinkle application. Int J Pharm Pharm Sci 2014;6:5–9.

Musallam Almousallam, Claudia Moia, Huijun Zhu. Development of nanostructured lipid carrier for dacarbazine delivery. Int Nano Lett 2015;5:241–8.

Shadab Khan, Ghazala Mulla, Kiran Bhise. Antipsoriatic potential of polyherbal paste. Res Rev: J Herbal Sci 2013; 8:20-32.

Bhise K, Khan S, Mulla G. Topical anti-psoriatic nanoparticulate drug delivery system. Int J Appl Pharm 2020;12:76-85.

KP Rao, Khaliq K, Kharat SS, Sagare P, Sk P. Preparation and evaluation of o/w cream for skin psoriasis. Int J Pharma Bio Sci 2010;1:1.

Mahapatra Annada Prasad, Mishra Dipak Kumar, Panda Prabhudutta. Formulation and evaluation of cream prepared from corton spardiflorus morong and their wounf healing activity. Int J Res Ayurveda Pharm 2012;3:803-7.

Shankar R, Sarangi B, Gupta R, Pathak K. Formulation and characterization of polyherbal cream for skin manifestations. J Asian Assocation Schools Pharm 2016;5:360–6.

Razi M, Khan U, Hussain M, Masood S, Ur S, Nazir R. Formulation design and in vitro characterization of etoricoxib cream for the treatment of rheumatoid arthritis. Iranian J Pharm Sci 2014;10:93–104.

Divya Pingili, Deepa Amminbavi, Archana Awasthi, Faisal Mohd Shamim Khan. Formulation, evaluation and in votro antibacterial screening of herbal gel containing Manilkara hexandra (roxb) dubard leaf extract. Int J

Pharm Sci Res 2018;9:702–7.

Kotta Ktanthi Kumar, K Sasikanth, M Sabareesh, N Dorabu. Formulation and evaluation of diacerien cream. Asian J Pharm Clin Res 2011;4:93-8.

Helal DA, El-rhman DABD, Abdel-halim SA, El-nabarawi MA. Formulation and evaluation of fluconazole topical gel. Int J Pharm Pharm Sci 2012;4:12–4.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.

Panasenko OM, Evgina SA, Driomina ES. Hypochlorite induces lipid peroxidation in blood lipoproteins and phospholipid liposomes. Free Radical Biol Med 1995;19:133-40.

Yokozawa T, Wang ST, Chen PC, Hattori M. Inhibition of nitric oxide release by an aqueous extract of tinospora tuberculata. Phytother Res 2000;14:51-3.

T Mosmann. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.

Colombo I, Sangiovanni E, Maggio R, Mattozzi C, Zava S, Corbett Y. HaCaT cells as a reliable in vitro differentiation model to dissect the inflammatory/repair response of human keratinocytes. Medistors of Inflammation 2017:1–12.

Li Z, Yu J, Liu L, Wei Z, Ehrlich ES. Coxsackievirus A16 infection induces neural cell and non-neural cell apoptosis in vitro. PLOS One 2014;9:1-10.

Takada M, Noguchi A, Sayama Y, Kurohane Kaneko Y, Ishikawa T. Inositol 1,4,5-trisphosphate receptor-mediated initial Ca(2+) mobilization constitutes a triggering signal for hydrogen peroxide-induced apoptosis in INS-1 β-cells. Biol Pharm Bull 2011;34:954-8.

Singhal M, Kansara N. Cassia tora linn cream inhibits psoriasis in mouse tail model. Pharm Crops 2012;3:1-6.

Manmohan Singhaland Niraj Kansara. Cassia tora linn cream inhibits ultraviolet-B-induced psoriasis in rats. ISRN Dermatol; 2012. p. 16.

Nakaguma H, Kambara T, Yamamoto T. Rat ultraviolet ray B photodermatitis: an experimental model of psoriasis vulgaris. Int J Exp Pathol 1995;76:65–73.

Nagamani M, Prahaladu P, Vijayababu PVSS, Ashalata K, Kusuma Kumari P, Kumari KL. Lipid peroxidation product as a marker of oxidative stress in psoriasis-a case-control study in north coastal Andhra Pradesh. IOSR J Dent Med Sci 2015;14:2279–861.

Ignacio NRS, Ferreira PLJ, Almeida BM, Kubelka FC. Nitric oxide production by murine peritoneal macrophages in vitro and in vivo treated with Phyllanthus tenellus extracts. J Ethnopharmacol 2001;74:181-7.



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