DEVELOPMENT AND EVALUATION OF ORAL SUSTAINED-RELEASE RANITIDINE DELIVERY SYSTEM BASED ON BACTERIAL NANOCELLULOSE MATERIAL PRODUCED BY KOMAGATAEIBACTER XYLINUS

  • Thanh Xuan Nguyen Director of Institute of Scientific Research and Applications (ISA), Hanoi Pedagogical University 2 (HPU2)
  • Mung Van Pham
  • Cuong Ba Cao

Abstract

Objective: The short biological half-life (2-3 h) and low bioavailability (50 %) of ranitidine (RAN) following oral administration favor development of a controlled release system. This study was aimed to develop and in vitro evaluate oral sustained-release RAN delivery system based on the bacterial nanocellulose material (BNM) produced by Komagataeibacter xylinus (K. xylinus) from selected culture media.


Methods: BNMs are biosynthesized by K. xylinus in the standard medium (SM) and coconut water (CW). RAN was loaded in BNMs by the absorption method. The structural and physicochemical properties of BNMs and BNMs-RAN were evaluated via swelling behavior, FTIR and FESEM techniques. Moreover, the effect of BNMs on RAN release profile and release kinetics was analyzed and evaluated.


Results: The amount of loaded RAN or entrapment efficacy for  BNM-CW is higher than for BNM-CW. The BNM-SM-RAN and BNM-CW-RAN exhibited a decreased initial burst release system followed by a prolonged RAN release up to 24 h in relation to the commercial tablets containing RAN. The RAN release from these formulations was found higher in the SGF medium than that of in SIF medium. RAN release from these formulations was found to follow Korsmeyer-Peppas model and diffusion sustained drug release mechanism. The sustained release of RAN from BNM-SM-RAN was slower than for RAN from BNM-CW-RAN, but the mechanism of sustained RAN release was the same.


Conclusion: Oral sustained-release RAN delivery system based on BNMs was successfully prepared and evaluated for various in vitro parameters. The biopolymers like BNM-SM and BNM-CW could be utilized to develop oral sustained RAN release dosage form.

Keywords: Bacterial nanocellulose material (BNM); Komagataeibacter xylinus (K. xylinus); Oral sustained-release; Ranitidine delivery system

Author Biographies

Thanh Xuan Nguyen, Director of Institute of Scientific Research and Applications (ISA), Hanoi Pedagogical University 2 (HPU2)

Thanh Xuan Nguyen is a main lecturer and a director of Institute of Scientific Research and Applications at Hanoi Pedagogical University 2 (HPU2). He received a Bachelor of Science in Biology and Chemistry in 2000 from HPU2, Vietnam. He was awarded the second prize of the Vietnamese scientific and technical creative competition in 1999 of the Vietnam Fund for Supporting Technological Creations (VIFOTEC), and the second prize of the Student Scientific Research in 1999 of the Ministry of Education and Training (MOET), Vietnam. He also received a Master of Science in Human and Animal Physiology in 2005 from the Hanoi National University of Education, Vietnam. In 2010, he won a full scholarship and became a PhD student of Biopharmaceutical Engineering at Huazhong University of Science and Technology (HUST), China. He received his PhD degree from HUST, in 2014. His research interests focus on Biopharmaceutical Engineering, application of bio-pharmaceutical engineering (new materials, bio-nanomaterials, multifunctional materials, and so on) to design and manufacture the intelligent carrier systems for drug delivery and the functionally active substances in the body.

Mung Van Pham

Mung Van Pham is a researcher and a lecturer of Hai Duong Central College of Pharmacy, 324 Nguyen Luong Bang, Hai Duong 170000, Vietnam.

Cuong Ba Cao

Cuong Ba Cao is a main lecturer and a researcher of Institute of Scientific Research and Applications (ISA), Hanoi Pedagogical University 2 (HPU2); Xuan Hoa, Phuc Yen, Vinh Phuc 280000, Vietnam.

References

1. Sharma C, Bhardwaj NK. Bacterial nanocellulose: Present status, biomedical applications and future perspectives. Mater Sci Eng C 2019;104:1-18.
2. Potzinger Y, Kralisch D, Fischer D. Bacterial nanocellulose: The future of controlled drug delivery. Ther Deliv 2017;8:753-61.
3. Muller A, Ni Z, Hessler N, Wesarg F, Muller FA, Kralisch D, et al. The biopolymer bacterial nanocellulose as drug delivery system: investigation of drug loading and release using the model protein albumin. J Pharm Sci 2013;102:579-92.
4. Huang L, Chen X, Nguyen TX, Tang H, Zhang L, Yang G. Nano-cellulose 3D-networks as controlled-release drug carriers. J Mater Chem B 2013;1:2976-84.
5. Moritz S, Wiegand C, Wesarg F, Hessler N, Muller FA, Kralisch D, et al. Active wound dressings based on bacterial nanocellulose as drug delivery system for octenidine. Int J Pharm 2014;471:45-55.
6. Wiegand C, Moritz S, Hessler N, Kralisch D, Wesarg F, Muller FA, et al. Antimicrobial functionalization of bacterial nanocellulose by loading with polihexanide and povidone-iodine. J Mater Sci Mater Med 2015;26:1-14.
7. Ullah H, Badshah M, Makila E, Salonen J, Shahbazi MA, Santos HA, et al. Fabrication, characterization and evaluation of bacterial cellulose-based capsule shells for oral drug delivery. Cellulose 2017;24:1445-54.
8. Badshah M, Ullah H, Khan SA, Park JK, Khan T. Preparation, characterization and in-vitro evaluation of bacterial cellulose matrices for oral drug delivery. Cellulose 2017;24:5041-52.
9. Subtaweesin C, Woraharn W, Taokaew S, Chiaoprakobkij N, Sereemaspun A, Phisalaphong M. Characteristics of curcumin-loaded bacterial cellulose films and anticancer properties against malignant melanoma skin cancer cells. Appl Sci 2018;8:1-15.
10. Morais ES, Silva NHCS, Sintra TE, Santos SAO, Neves BM, Almeida IF, et al. Anti-inflammatory and antioxidant nanostructured cellulose membranes loaded with phenolic-based ionic liquids for cutaneous application. Carbohydr Polym 2019;206:187-97.
11. Marabathuni VJ, Deveswaran R, Bharath S, Basavaraj BV, Madhavan BV. Design and optimization of multiparticulate gastroretentive delivery system of ranitidine hydrochloride. Int J Pharm Pharm Sci 2012;4:597-603.
12. Patel R, Bathe RS, Khobragade D, Jadhav S. Formulation and evaluation of gastroretentive beads of ranitidine hydrochloride. Int J Pharm Pharm Sci 2014;6:237-42.
13. Maharjan R, Subedi G. Formulation and evaluation of floating in situ gel of ranitidine using natural polymers. Int J Pharm Pharm Sci 2014;6:205-9.
14. Dixit GR, Chavhan JI, Upadhye KP, Misra S. Formulation and characterization of mucoadhesive buccal film of ranitidine hydrochloride using sterculia Foetida gum as polymer. Asian J Pharm Clin Res 2015;8:68-71.
15. Etman ME, Mahmoud EH, Galal S, Nada AH. Floating ranitidine microparticulates: Development and in vitro evaluation. Int J App Pharm 2016;8:1-9.
16. Rajeswari S, Kudamala S, Murthy KVR. Development, formulation and evaluation of a bilayer gastric retentive floating tablets of ranitidine HCl and clarithromycin using natural polymers. Int J Pharm Pharm Sci 2017;9:164-77.
17. Sahu VK, Sharma N, Sahu PK, Saraf SA. Formulation and evaluation of floating-mucoadhesive microspheres of novel natural polysaccharide for site specific delivery of ranitidine hydrochloride. Int J App Pharm 2017;9:15-9.
18. Maraie NK, Salman ZD, Yousif NZ. Design and characterization of oroslippery buoyant tablets for ranitidine hydrochloride. Asian J Pharm Clin Res 2018;11:143-9.
19. Darbasizadeh B, Motasadizadeh H, Foroughi-Nia B, Farhadnejad H. Tripolyphosphate-crosslinked chitosan/poly (ethylene oxide) electrospun nanofibrous mats as a floating gastro-retentive delivery system for ranitidine hydrochloride. J Pharm Biomed Anal 2018;153:63-75.
20. Torne SR, Sheela A, Sarada NC. Ranitidine controlled release anti-reflux suspension for gastro-oesophageal reflux disease and it’s in vitro evaluation. Int J App Pharm 2019;11:74-81.
21. Jafar M, Mohsin AA, Khalid MS, Alshahrani AM, Alkhateeb FS, Alqarni AS. Ranitidine hydrochloride stomach specific bouyant microsponge: Preparation, in-vitro characterization, and in-vivo anti-ulcer activity. J Drug Deliv Sci Technol 2020;55:1-8.
22. Xu J, Tan X, Chen L, Li X, Xie F. Starch/microcrystalline cellulose hybrid gels as gastric-floating drug delivery systems. Carbohydr Polym 2019;215:151-9.
23. Hestrin S, Schramm M. Synthesis of cellulose by Acetobacter xylinum, 2. Preparation of freeze-dried cells capable of polymerizing glucose to cellulose. Biochem J 1954;58:345-52.
24. Zhang Y, Huo M, Zhou J, Zou A, Li W, Yao C, Xie S. DDSolver: An add-in program for modeling and comparison of drug dissolution profiles. AAPS J 2010;12:263-71.
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Nguyen, T., Pham, M., & Cao, C. (2020). DEVELOPMENT AND EVALUATION OF ORAL SUSTAINED-RELEASE RANITIDINE DELIVERY SYSTEM BASED ON BACTERIAL NANOCELLULOSE MATERIAL PRODUCED BY KOMAGATAEIBACTER XYLINUS. International Journal of Applied Pharmaceutics, 12(3). Retrieved from https://innovareacademics.in/journals/index.php/ijap/article/view/37218
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