THE RELATIONSHIP BETWEEN GDF-9 AND BMP-15 SERUM AND FOLLICULAR FLUID AND THE QUALITY OF OOCYTES IN WOMEN WHO UNDERGO AN IVF CYCLE

  • ZAINAL ARIFIN Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Yasmin IVF Clinic Center, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia
  • KANADI SUMAPRADJA Human Reproduction, Infertility and Family Planning Program Research Center, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Yasmin IVF Clinic Center, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia
  • UPIK ANGGRAENI Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Yasmin IVF Clinic Center, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia
  • NAYLAH MUNA Human Reproduction, Infertility and Family Planning Program Research Center, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
  • BUDI WIWEKO Human Reproduction, Infertility and Family Planning Program Research Center, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Yasmin IVF Clinic Center, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia

Abstract

Objective: The quality of the embryo is greatly influenced by the quality of the oocytes; oocyte-secreted factors (OSFs), which include GDF-9 and BMP-15, play an important role in folliculogenesis. This study was to determine the relationship between GDF-9 and BMP-15 serum within the follicular fluid in order to predict the quality of oocytes in women undergoing In vitro Fertilization (IVF).


Methods: We collected 30 samples of blood serum and 30 samples of follicular fluid on the day of ovum pickup (OPU), and examined GDF-9 and BMP-15 using ELISA kits. Analysis by Pearson and a partial-correlation was conducted to analyze the correlation between the concentration of GDF-9 and BMP-15 in serum and follicular fluid with general physiological parameters, such as maturation and fertilization rates.


Results: The mean age of the subjects was 35,0(26,0-39,0) years. There was no statistical correlation between GDF-9 serum and follicular fluid (p=0.245); but there was a correlation between BMP-15 serum and follicular fluid (p=0.001). Average level of GDF-9 in the follicular fluid was 163,0 pg/ml (48,0-537), and average level in the serum was 260.33 pg/ml±121,82; average levels of BMP-15 in the follicular fluid was 58.30pg/ml±31,54 and average levels of BMP-15 in the serum was 74.20 pg/ml (1,0–610).


Conclusion: There were no correlations between levels of GDF-9 serum-FF and BMP-15-FF, and maturation rates and fertilization rates. There was a negative correlation between BMP-15 serum and maturation rates.

Keywords: BMP-15, Fertilization rate, Follicular fluid, GDF-9, Maturation rate

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ARIFIN, Z., SUMAPRADJA, K., ANGGRAENI, U., MUNA, N., & WIWEKO, B. (2020). THE RELATIONSHIP BETWEEN GDF-9 AND BMP-15 SERUM AND FOLLICULAR FLUID AND THE QUALITY OF OOCYTES IN WOMEN WHO UNDERGO AN IVF CYCLE. International Journal of Applied Pharmaceutics, 12(3). Retrieved from https://innovareacademics.in/journals/index.php/ijap/article/view/39463
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