• EVY FENNY VERONICA Faculty of Pharmacy, Sanata Dharma University, Yogyakarta, Indonesia
  • RINI DWIASTUTI Faculty of Pharmacy, Sanata Dharma University, Yogyakarta, Indonesia



Leucaena leaves, Gel, Wound healing, Gelling agent, Humectant


Objective: The aim of this research is to formulate and evaluate the wound healing gel of Leucaena leucocephala leaves extract.

Methods: In this research, the extract of Leucaena leaves was formulated into wound healing gel by using a variation of the concentration of Carbopol as a gelling agent and Propylene glycol as a humectant. Afterward, the gel's physical properties (pH, viscosity, spreadability), stability, and sterility were tested. The wound healing activity was evaluated by making excision wounds on the Wistar Albino rat's back, and then the gels were applied to the wound every day. The wound's size was measured and counted as the percentage of wound closure.

Results: The result showed that Formula 4 (contains 1.5% of Carbopol and 12% of Propylene glycol) has the best physical characteristics and wound healing activity. Formula 4 showed 100% wound closure on the 11th day of the treatment, while the negative control only reached 49.12%. The statistical parameter with the p-value<0.05 stated that they are significantly different.

Conclusion: This research demonstrated that gel with Leucaena leaf extract has good physical characteristics, and it can significantly improve the wound healing process.


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Lei J, Sun L, Li P, Zhu C, Lin Z. The wound dressings and their applications in wound healing and management. Heal Sci J. 2019;13;3(662):1-8.

Dhivya S, Padma VV, Santhini E. Wound dressings-a review. Biomedicine. 2015;5(4):24-8.

Sorg H, Tilkorn DJ, Hager S, Hauser J, Mirastschijski U. Skin wound healing: an update on the current knowledge and concepts. Eur Surg Res. 2017;58(1-2):81-94. doi: 10.1159/000454919, PMID 27974711.

Vowden K, Vowden P. Wound dressings: principles and practice. Surgery (Oxford). 2014;32(9):462-7. doi: 10.1016/j.mpsur.2014.07.001.

FDA. FDA executive summary: classification of wound dressings combined with drugs. In: Meeting of the General and Plastic Surgery Devices Advisory Panel 2016. p. 1-83.

Saghazadeh S, Rinoldi C, Schot M, Kashaf SS, Sharifi F, Jalilian E, Nuutila K, Giatsidis G, Mostafalu P, Derakhshandeh H, Yue K, Swieszkowski W, Memic A, Tamayol A, Khademhosseini A. Drug delivery systems and materials for wound healing applications. Adv Drug Deliv Rev. 2018;127(Mar 1):138-66. doi: 10.1016/j.addr.2018.04.008, PMID 29626550.

Sarabahi S. Recent advances in topical wound care. Indian J Plast Surg. 2012;45(2):379-87. doi: 10.4103/0970-0358.101321, PMID 23162238.

Amirthalingam S, Yi KS, Ching LT, Mun NY. Topical antibacterials and global challenges on resistance development. Trop J Pharm Res. 2015;14(5):919-24. doi: 10.4314/tjpr.v14i5.24.

Sartinah A, Astuti P. Wahyuono S. Isolasi dan Identifikasi Senyawa Antibakteri dari Daun Petai Cina (Leucaena leucocephala (Lam.) de Wit.). Maj Obat TraDIS. 2010;15(3).

Zayed MZ, Samling B. Phytochemical constituents of the leaves of Leucaena leucocephala from Malaysia. Int J Pharm Pharm Sci. 2016;8(12):174-9. doi: 10.22159/ijpps.2016v8i12.11582.

Chatchanayuenyong R, Sujayanont P. Evaluation of the anti-proliferation and anti-migration effects of Leucaena leucocephala and Dolichandrone serrulata ethanolic extracts against human cervical cancer cell line. Phcog Mag. 2020;16(68). doi: 10.4103/pm.pm_327_19.

Eritriana RE, Rosiana AH, Tantri Y, Ekayanti E, Lamtoro EED. (Leucaena leucocephala L.) sebagai Alternatif Penyembuhan Luka Abrasi. J Penelit Kesehat Suara Forikes. 2019;10(2):290-4.

Budiman A, Muharam MAC, Maulida AC, Aulifa DL. Formulation of gel from Gynura segetum extract and its activity on burn wound healing. Int J App Pharm. 2021;13(2):269-71. doi: 10.22159/ijap.2021v13i2.40438.

Widyaningsih W, Salamah N, Maulida QF. Gel formulation of ethanol extract of mangosteen peel (Garcinia mangostana L.) as a medication for burns in Wistar rats. Indones J Heal J 2016;8(2):110-7.

Morsy MA, Abdel-Latif RG, Nair AB, Venugopala KN, Ahmed AF, Elsewedy HS, Shehata TM. Preparation and evaluation of atorvastatin-loaded nanoemulgel on wound-healing efficacy. Pharmaceutics. 2019;11(11):1-15. doi: 10.3390/pharmaceutics11110609, PMID 31766305.

Ermawati DE, Ramadhani CI. Formulation of anti-acne gel of moringa oleifera, l. ethanolic extract and antibacterial test on staphylococcus epidermidis. Majalah Farmaseutik 2020;16(2):154-62. doi: 10.22146/farmaseutik.v16i2.50319.

Sanders ER. Aseptic laboratory techniques: plating methods. J Vis Exp. 2012;63(63):e3064. doi: 10.3791/3064, PMID 22617405.

Lodhi S, Vadnere GP. Relevance and perspectives of experimental wound models in wound healing research. Asian J Pharm Clin Res. 2017;10(7):68-75.

Liu H, Lin S, Xiao D, Zheng X, Gu Y, Guo S. Evaluation of the wound healing potential of Resina Draconis (Dracaena cochinchinensis) in animal models. Evid Based Complement Alternat Med. 2013;2013:709865. doi: 10.1155/2013/709865, PMID 23762154.

Sabat PK, Pradhan SP, Patro R. Evaluation of excisional and incisional wound healing activity of electrohomeopathic drug (spagyric essence) green electricity in rats. Int J Pharm Pharm Sci. 2020;12(10):72-5. doi: 10.22159/ijpps.2020v12i10.38674.

Fitrian A. Efek angiogenesis gel ekstrak daun lamtoro (Leucaena leucocephala) pada Luka Insisi tikus. JBP. 2018;20(1):22. doi: 10.20473/jbp.v20i1.2018.22-32.

Deivasigamani R. Phytochemical analysis of Leucaena leucocephala on various extracts. J Phytopharmacol. 2018;7(6):480-2. doi: 10.31254/phyto.2018.7605.

Lantis JC, Paredes JA. Topical wound care treatment and indications for their use. New York: international springer publishing. Agriculturists; 2018. p. 281-304.

R Varges P, M Costa C, S Fonseca B, F Naccache M, De Souza Mendes P. Rheological characterization of carbopol® dispersions in water and in water/glycerol solutions. Fluids. 2019;4(1):1-20. doi: 10.3390/fluids4010003.

Purnamawati S, Indrastuti N, Danarti R, Saefudin T. The role of moisturizers in addressing various kinds of dermatitis: a review. Clin Med Res. 2017;15(3-4):75-87. doi: 10.3121/cmr.2017.1363, PMID 29229630.

Jones EM, Cochrane CA, Percival SL. The effect of pH on the extracellular matrix and biofilms. Adv Wound Care. 2015;4(7):431-9. doi: 10.1089/wound.2014.0538, PMID 26155386.

Baron JM, Glatz M, Proksch E. Optimal support of wound healing: new insights. Dermatology. 2020;236(6):593-600. doi: 10.1159/000505291, PMID 31955162.

Safitri FI, Nawangsari D, Febrina D. Overview: application of carbopol 940. In: Gel international conference on health and medical sciences; 2020. p. 80-4.

USP. Topical and transdermal drug products. The U State Pharmacopeial/Natl Formul Pharmacopeial Forum; 2009. p. 750-64.

World Health Organization (WHO). Topical semi-solid dosage forms. Int Pharmacopoeia; 2019. p. 2-4.

Sieniawska E, Baj T. Tannins. In: Pharmacognosy: fundamentals, applications and strategy. Elsevier Inc; 2017. p. 199-232.

Zarina Z, Ghazali CMR, Sam ST. Characterization analysis for leaves of Leucaena leucocephala by using phytochemical screening assay. AIP Conf Proc; 2017. p. 1885.

Aslam MS, Ahmad MS, Riaz H, Raza SA, Hussain S, Qureshi OS. Role of flavonoids as wound healing agent. Phytochemicals-Source Antioxid Role Dis Prev Integr; 2018. p. 95-102.

Majewska I, Gendaszewska Darmach E. Proangiogenic activity of plant extracts in accelerating wound healing - a new face of old phytomedicines. Acta Biochim Pol. 2011;58(4):449-60. doi: 10.18388/abp.2011_2210, PMID 22030557.



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