• MANJUSHA A. BHANGE Department of Pharmaceutics, Datta Meghe College of Pharmacy, Datta Meghe Institute of Higher Education and Research, (Deemed to be University), Sawangi (Meghe), Wardha, Maharashtra, India 442001
  • ANIL PETHE Department of Pharmaceutics, Datta Meghe College of Pharmacy, Datta Meghe Institute of Higher Education and Research, (Deemed to be University), Sawangi (Meghe), Wardha, Maharashtra, India 442001
  • ANKITA HADKE Department of Pharmaceutics, Datta Meghe College of Pharmacy, Datta Meghe Institute of Higher Education and Research, (Deemed to be University), Sawangi (Meghe), Wardha, Maharashtra, India 442001



Ferulic acid, Conjugation, Complex formation, Solvent evaporation method, Nanoparticle, Drug targeted system, Bioavailability


Objective: The objective of the study was to design and formulate ferulic acid phytosomes converted in to functionalised soft nanoparticles by using solvent evaporation method to increase resistance time, improve bioavailability, and half-life of ferulic acid.

Methods: Ferulic acid is a BCS-II drug,  which has low solubility and high permeability. The functionalised soft nanoparticles was prepared by the solvent evaporation method followed by the particle size and zeta potential, FTIR, PXRD, SEM. It indicates good result for the complexation rate. PXRD showed good powder diffraction results with having good flow property. Particle size and zeta potential had a good result of -12.05±120 improved by the cationic polymer. The complex was evaluated by the study of Drug loading, Entrapment efficiency, Histopathological study and mucoadhesive property for the final formulation of the microspheres system. Also formulation were evaluated for the In-vitro drug dissolution study for rate of extent of drug release. Ex-vivo drug diffusion study by using goat nasal mucosa using pH 6.6 for evaluating rate of extent of drug diffusion through nasal mucosa.

Results: The results of the characterization studies indicated the designing of functionalised phytosomal soft nanoparticles. The functionalised phytosomal soft nanoparticles (FPSN) particle size and zeta potential had a good result of -12.05±120. The FTIR spectra of complex showed characteristic peak at 3652.8 cm-1 (OH-stretching) which indicate that the shifting and interaction between the drug and phospholipids SPC 3. PXRD, SEM, in-vitro dissolution showed good powder diffraction results with having good flow property. The complex is evaluated by the study of Drug loading. Also formulation were evaluated for the In vitro drug   dissolution study for rate of extent of drug release. The result ofabove studies was Drug loading increased at 44.42 %. ex-vivo permeation study FALC-MS showed characteristic in the drug diffusion at 80.04 % which indicate that the drug had increases its aqueous solubility and also change with the structural morphology.

Conclusion: It can be concluded that the ferulic acid phytosomal soft nanoparticles enhance the solubility of the ferulic acid and increased the bioavailability and retention time to target the liver cancer.

Keywords: Ferulic Acid,  Conjugation, Complex Formation, Solvent evaporation technique, Nanoparticle, Drug targeted systems, Bioavailability.


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H Oya Alpar 1, S Somavarapu, K N Atuah, V W Bramwell. Biodegradable mucoadhesive particulates for nasal and pulmonary antigen and DNA delivery. DOI: 10.1016/j.addr.2004.09.004.

Cetin Tas 1, Cansel Kose Ozkan, Ayhan Savaser, Yalcin Ozkan, Umut Tasdemir, Hikmet Altunay. Nasal absorption of metoclopramide from different Carbopol 981 based formulations: In vitro, ex vivo and in vivo evaluation. DOI: 10.1016/j.ejpb.2006.05.017.

Chandna Amit, Batra Deepa, Kakar Satindar , Singh Ramandeep. A review on target drug delivery: Magnetic microspheres. DOI:10.1016/S2221-6189(13)60125-0.

Ksy Hemant, Raizaday* Abhay, Sivadasu Praveen, Uniyal Swati, Kumar S. Hemanth. Cancer nanotechnology: nanoparticulate drug delivery for the treatement of cancer. Int j pharm pharm sci, vol 7, issue 3, 40-46. ISSN-0975-1491.

Chandan A, Batra D, Kakar R. Singh A. Review on target drug delivery: magnetic microspheres. 10.1016/S2221-6189(13)60125-0.

O. Cota-Arriola, M. Plascencia-Jatomea, J. Lizardi-Mendoza, R. M. Robles-Sánchez, J. M. Ezquerra-Brauer, J. Ruíz-García, Preparation Of Chitosan Matrices With Ferulic Acid: Physicochemical Characterization And Relationship On The Growth Of Aspergillus Parasiticus. Doi.Org/10.1080/19476337.2016.1213317.

Senthil Kumar Raju1* Anandakumar Karunakaran2, Shridharshini Kumar1 , Praveen Sekar1 , Maruthamuthu Murugesan1 , Mohanapriya Karthikeyan1. Biogenic Synthesis Of Copper Nanoparticles And Their Biological Applications: An Overview, Int J Pharm Pharm Sci, ISSN: 0975-1491 Vol 14, Issue 3, 2022.

Khan Y. Yunus, Vasanti Suvarna. Liposomes containing phytochemicals for cancer treatment-an update. Int j curr pharm res, vol 9, issue 1, 20-24.

Telange DR, Patil AT, Pethe AM, Fegade H, Anand S, Dave VS. Formulation and characterization of an apigenin-phospholipid phytosome (APLC) for improved solubility, in vivo bioavailability, and antioxidant potential. Eur J Pharm Sci. doi: 10.1016/j.ejps.2016.12.009.

V. Sivapriya, S. Ponnarmadha, N. Abdul Azeez, V. Sudarshan A. Deepa. Novel Nanocarriers For Ethnopharmacological Formulations, Int J App Pharm, Vol 10, Issue 4, 2018, 26-30. DOI: 10.22159/ijap.2018v10i4.26081.

Gupta S, Samanta M K, Raichur A M. Dual-drug delivery system based on in situ gel-forming nanosuspension of forskolin to enhance antiglaucoma efficacy. AAPS PharmSciTech. Mar;11(1):322-35. doi: 10.1208/s12249-010-9388-x.

Hasçiçek C, Gönül N, Erk N. Mucoadhesive microspheres containing gentamicin sulfate for nasal administration: preparation and in vitro characterization. Farmaco. 2003 Jan;58(1):11-6. doi: 10.1016/S0014-827X(02)00004-6.

He P, Davis SS, Illum L. Sustained release chitosan microspheres prepared by novel spray drying methods. J Micro encapsul. 1999 May-Jun;16(3):343-55. doi: 10.1080/026520499289068. PMID: 10340219.

Hermens, W.A.J.J., Merkus, F.W.H.M. The Influence of Drugs on Nasal Ciliary Movement. Pharm Res 4, 445–449 (1987).

Illum Lisbeth, Is nose-to-brain transport of drugs in man a reality?, Journal of Pharmacy and Pharmacology, Volume 56, Issue 1, January 2004, Pages 3–17,

Illum L. Nasal drug delivery--possibilities, problems and solutions. J Control Release. 2003 Feb 21;87(1-3):187-98. doi: 10.1016/s0168-3659(02)00363-2. PMID: 12618035.

Kakar Satinder , Batra Deepa, Singh Ramandeep, Nautiyal Ujjwal, Magnetic microspheres as magical novel drug delivery system: A review, Journal of Acute Disease, doi:10.1016/S2221-6189(13)60087-6.

G Karthivashan, MJ Masarudin, AU Kura, F Abas, S Fakurazi. Optimization, formulation, and characterization of multiflavonoids-loaded flavanosome by bulk or sequential technique. Int J Nanomedicine. 2016 Jul 27;11:3417-34. doi: 10.2147/IJN.S112045.

Koyani V, Dedaka P, Matholiya P. Microspheres for intransal deliver system: as review. J of informa health care. ISSN: 2249-1759 & E-ISSN: 2249-1767, Volume 2, Issue 1, 2014.

ME Meredith, TS Salameh, WA Banks. Intranasal delivery of proteins and peptides in treatment of neurodegenerative diseases. AAPS J. 2015; 17(4):1-8. doi:10.1208/s12248-015-9719-7.

Mistry A, Glud SZ, Jorgen K, Randel J, Howard KA, Stolnik S, Illum L. Effect of physicochemical properties on intranasal nanoparticle transit into murine olfactory epithelium. J. Drug Target. 2009; 17(7): 543-552. doi: 10.1080/10611860903055470.

Miyake MM, M.D, Bleier BS. The blood brain barrier and nasal drug delivery to the central nervous system. 2015; 29(2): 124-127. doi: 10.2500/ajra.2015.29.4149.

Pristis E, Dhommat R, Jain A, Swami Challa VG, Shaheen M, Khan W. Intranasal delivery of nanoparticle encapsulated tarenflurbil: A potential brain targeting strategy for Alzheimer’s diseases. Eur. J. Pharm. Sci. 2016 May; 1-36.doi:10.1016/j.ejps.2016.05.012.

Y. Murata, E. Miyamoto, S. Kawashima. Additive effect of clondroitin sulphate and chitosan on drug release from calcium induced alginate gel beads. J. Control. Release. 1996; 38(2-3):101-108. doi: 10.1016/0168-3659(95)00098-4.

Patel MR, Patel RB, Bhatt KK. Patel BG, Gaikwad RV. Paliperidone microemulsion for nose-to-brain target drug delivery system: pharmacodynamic and pharmacokinetics evaluation. Drug Deliv. 2014 May 28; 23(1): 346-354.

Patil SB, Sawant KK. Chitosan microspheres as a delivery system for nasal insufflations. Colloids Surf. B. 2011; 84: 384-389. doi:10.1016/j.colsurfb.2011.01.030.

Jeevana Jyothi B*, Mary Ragalatha P. Development And In Vitro Evaluation of Phytosomes of Naringin, Asian J Pharm Clin Res. DOI:10.22159/ajpcr.2019.v12i9.34798. Vol 12, Issue 9, 2019.

Robinson N, Garabowski P, Rehman I. Alzheimer disease pathogenesis: Is there a role of folate. Mech. Ageing Dev. 2017 Oct 5;86-94. doi: 10.1016/j.mad.2017.10.001.

Shah S, Qaqish R, Patel V, Amiji M. Evaluation of the factors influencing stomach specific delivery of antibacterial agents for helicobactor pylori infection. J. Pharm. Pharmacol. 1999 Jun; 51(6): 667-672. doi:10.1211/0022357991772952.

Shingaki T, Inoue D, Furubayashi T, Sakane T. Transnasal delivery of methotrexate to brain tumors in rats: a new strategy for brain tumor chemotherapy. Mol. Pharm. 2010 Aug; 7(5): 452. doi:10.1021/mp900275s.

Trombino S, Serini S, Fiorella N, Celleno L, Ando S. Antioxidant effect of ferulic acid in isolated membrane and intact cells: synergistic interaction with α-Tocopherol, β-carotene and ascorbic acid. J Agric Food Chem. 2004 Mar 20; 52(8): 2411–2420. doi.10.1021/jf0303924.



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