PHYTOCHEMICAL SCREENING, ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF ACTIVE COMPOUNDS FROM HEMIDESMUS INDICUS
Keywords:Hemidesmus indicus, Phytochemical screening, Fingerprinting, HPLC, Antioxidant activity, Antibacterial activity
Objective: Ayurveda and Herbal medicine are two important forms of alternative medicine that are widely available in India. This work is mainly concerned with the identification of the compounds by HPLC analysis, phytochemical analysis, and antioxidant and antibacterial properties of Indian medicinal plant Hemidesmus indicus root extracts.
Methods: In the present work the compounds of Hemidesmus indicus were identified from ethanolic extract, by HPLC. Different extracts (Hexane, Ethylacetate, and Ethanol) of its root were used for the determination of the antioxidant activity by using DPPH method. These extracts of Hemidesmus indicus were also checked for antibacterial activity using disc diffusion method against pathogenic bacteria such as Bacillus megaterium, staphylococcus aureus, pseudomonas aeruginosa and Klebsila pneumonia.
Results: In the present work two (2-hydroxy-4-methoxy benzaldehyde and 2-hydroxy-4-methoxybenzoic acid) compounds of Hemidesmus indicus were identified from ethanolic extract, by HPLC technique which were used as medicine for antioxidant and antibacterial activity. The phytochemical analysis of H. indicus root extracts showed the presence of phenols, glycosides, flavonoids and steroids. All root extracts has very well anti-oxidant and antibacterial activity
Conclusion: In vitro study indicates that these plant extracts is a significant source of natural antioxidant, which might be helpful in preventing the progress of various oxidative stresses and presence of alkaloids and polyphenols in higher concentration than the other phytochemicals suggests that these compound can be responsible for the antibacterial activity.
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