• Manoharan Mohana Department of Biochemistry, Biotechnology, and Bioinformatics, Avinashilingam Institute for Home Science and Higher Education for Women University, Coimbatore 641043, Tamil Nadu, India
  • Palghat Raghunathan Padma Department of Biochemistry, Biotechnology, and Bioinformatics, Avinashilingam Institute for Home Science and Higher Education for Women University, Coimbatore 641043, Tamil Nadu, India


Objective: Bacopa monnieri is a herb which has been used in Indian traditional medicine for a long period. Bacosides, the major constituents of Bacopa monnieri, have antioxidant, anticancer and memory enhancing properties.

Methods: The bacoside fraction was isolated from the dried powder of the aerial parts of Bacopa monnieri. The free radical scavenging activity of the bacoside fraction was determined by different assays (DPPH, ABTS, SOâ—, NOâ—, OHâ— and H2O2) at concentrations ranging from 5 µg to 200 µg.

Results: The scavenging activity was increased in a dose-dependent manner from 5µg to 50 µg and a plateau in activity was observed from concentrations higher than 50 µg of the bacoside fraction.

Conclusion: Higher concentrations did not show further increase in scavenging activity. Various in vivo and in vitro studies are needed to assess the medicinal potential of the bacoside fraction

Keywords: Bacopa monnieri, bacoside fraction, and free radical scavenging activity.


1. Satyavati GV, Raina MK, Sharma M. Chemical investigation of Herpestis monniera Linn. (Brahmi). Indian J Pharmacol 1976;21:303-4.
2. Sivaprabha J, Sumathi S, Dharani B, Padma PR. Radical scavenging activity of leaves and rhizomes of Curcuma amada. Int J Pharm Res Dev 2011;3:167-74.
3. Zhou Y, Shen YH, Zhang C, Su J, Liu RH, Zhang WD. Triterpene Saponins from Bacopa monnieri and their antidepressant effects in two mice models. J Nat Prod 2007;70:652–5.
4. Calabrese C, Gregory WL, Leo M, Kraemer D, Bone K, Oken, B. Effects of a Standardized Bacopa monnieri extract on cognitive performance, anxiety, and depression in the elderly: a randomized, double-blind, placebo-controlled trial. J Alternative Complementary Med 2008;14:707–13.
5. Gohil KJ, Patel JA. A review on Bacopa monniera: current research and future prospects. Int J Green Pharm 2011;4. Doi:10.4103/0973-8258.62156. [Article in Press]
6. Chatterji N, Rastogi RP, Dhar ML. Chemical examination of Bacopa monniera Wettst: parti-isolation of chemical constituents. India J Chem 1963;1:212-5.
7. Rastogi S, Kulshreshtha DK. Bacoside A2–a triterpenoid saponin from Bacopa monniera. Indian J Chem 1998;38:353-6.
8. Rastogi S, Pal R, Kulshreshtha DK. Bacoside A3–a triterpenoid saponin from Bacopa monniera. Phytochemicals 1994;36:133–7.
9. Hou CC, Lin SJ, Cheng JT, Hsu FL. Bacopaside III, Bacopasaponin G, and Bacopasides A, B, and C from Bacopa monniera. J Nat Prod 2002;65:1759–63.
10. Chakravarty AK, Sarkar T, Nakane T, Kawahara N, Masuda K. New Phenylethanoid glycosides from Bacopa monniera. Chem Pharm Bull 2002;50:1616-8.
11. Chakravarty AK, Garai S, Masuda K, Nakane T, Kawahara N. Bacopasides III-IVP; three new triterpenoid glycosides from Bacopa monniera. Chem Pharm Bull 2003;51:215-7.
12. Zhou Y, Shen YH, Zhang C, Zhang WD. Chemical constituents of Bacopa monnieri. Chem Nat Compd 2007;43:355-7.
13. Jain P, Kulshereshtha DK. Bacoside A1, a minor saponin from Bacopa monniera. Phytochemicals 1993;33:449-51.
14. Croteau R, Kutchan TM, Lewis NG. Natural products (Secondary metabolites). In: Buchanan B, Gruissem W, Jones R. eds. Biochemistry and molecular biology of plants, Rockville, MD; American Society of Plant Physiologists; 2000. p. 1250-318.
15. Nui TP, Wattanathorn J, Muchimapura S, Un TT, Piyavhatkul N, Rangseekajee P, et al. Effects of 12-week bacopa monnieri consumption on attention, cognitive processing, working memory, and functions of both cholinergic and monoaminergic systems in healthy elderly volunteers. J Evidence-Based Complementary Altern Med 2012. Doi:10.1155/2012/606424. [Article in Press]
16. Saraf KM, Prabhakar S, Khanduja KL, Anand A. Bacopa monnieri attenuates scopolamine-induced impairment of spatial memory in mice. J Evidence-Based Complementary Altern Med 2011. Doi:10.1093/ecam/neq038. [Article in Press]
17. Mukherjee S, Dugad S, Bhandare R, Powar N, Jagtap S, Pawar PK, et al. Evaluation of comparative fee radical quenching potential of Brahmi (Bacopa monnieri) and Mandookparni (Centella asiatica). AYU 2013;32:258-64.
18. Radha P. Antioxidant responses evoked in vitro and in vivo by Bacopa monnieri leaf Extracts. A thesis submitted to Avinashilingam University for women, coimbatore in partial fulfillment of the requirement for the degree of Doctor of Philosophy in Biochemistry; 2010.
19. Kahol AP, Singh T, Tandon S, Gupta MM, Khanuja SPS. Process for the preparation of the extract rich in bacosides from the herb Bacopa monnieri. US Patent, US 6833143B1; 2004.
20. Mensor LL, Menezer FS, Leitae GC, Resi AS, Dossantos TC, Coube CS, et al. Scavenging of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother Res 2001;15:127-30.
21. Shirwaikar A, Ram HNA, Mohapatra P. Antioxidant and antiulcer activity of aqueous extract of the polyherbal formulation. Indian J Exp Biol 2006;44:474-80.
22. Winterbourn CC, Hawkins RW, Brain M, Carell RW. The estimation of red cell superoxide dismutase activity. J Lab Clin Med 1975;8:337-41.
23. Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite, and (15N) nitrate in a biological fluid. Anal Biochem 1982;126:131-8.
24. Elizabeth K, Rao HNA. Oxygen radical scavenging activity of curcumin. Int J Pharm 1990;58:237-40.
25. Ruch RJ, Cheng S, Klaunig JE. Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 1989;10:1003-8.
26. Chen Y, Miao Y, Huang L, Li J, Sun H, Zhao Y, et al. Antioxidant activities of saponins extracted from Radix trichosanthis: an in vivo and in vitro evaluation. BMC Complementary Altern Med 2014;14:86-93.
27. Ayaz M, Junaid M, Ahmed J, Ullah F, Sadiq A, Ahmad S. Phenolic contents, antioxidant and anticholinesterase potentials of crude extract, subsequent fractions, and crude saponins from Polygonum hydropiper L. BMC Complementary Altern Med 2014;14:145-53.
28. Sivaprabha J, Sumathi S, Dharani B, Padma PR. Radical scavenging activity of leaves and rhizomes of Curcuma amada. Int J Pharm Res Dev 2011;3:167-74.
29. Ramesh BN, Girish TK, Raghavendra RH, Naidu KA, Rao UJSP, Rao KS. Comparative study on antioxidant and anti-inflammatory activities of Caesalpinia crista and Centella asiatica leaf extracts. J Pharm Bioallied Sci 2014;6:86-91.
30. Zarena AS, Gopal S, Vineeth R. Antioxidant, antibacterial and cytoprotective activity of agathi leaf protein. J Anal Methods Chem 2014. Doi:10.1155/2014/989543. [Article in Press]
31. Murugan S, Selvaraj S, Viswanathan PK. In vitro antioxidant activities of Solanum surattense leaf extract. Asian Pac J Trop Biomed 2013;3:28-34.
32. Narasimhan MK, Pavithra SK, Krishnan V, Chandrasekaran M. In vitro analysis of antioxidant, antimicrobial and antiproliferative activity of Enteromorpha antenna, Enteromorpha linza and Gracilaria corticata extracts. Jundishapur J Natl Pharm Prod 2013;8:151-9.
33. Nartey ET, Ofosuhene M, Kudzi W, Agbale CM. Antioxidant and gastric cytoprotective prostaglandins properties of Cassia sieberiana roots bark extract as an anti-ulcerogenic agent. BMC Complementary Altern Med 2012;12:65-74.
34. Oboh G, Ademosun AO. Characterization of the antioxidant properties of phenolic extracts from some citrus peels. J Food Sci Technol 2012;49:729-36.
35. Gul MZ, Bhakshu LM, Ahmad F, Kondapi AK, Qureshi IA, Ghazi1 IA. Evaluation of Abelmoschus moschatus extracts for antioxidant, free radical scavenging, antimicrobial and antiproliferative activities using in vitro assays. BMC Complementary Altern Med 2011;11:64-75.
36. Chan CF, Huang WY, Guo HY, Wang BR. Potent antioxidative and UVB protective effect of water extract of Eclipta prostrata L. Sci World J 2014. Doi:10.1155/2014/759039. [Article in Press]
37. Chipiti T, Ibrahim MA, Koorbanally NA. In vitro antioxidant activities of leaf and root extracts of Albizia antunesiana harms. Acta Pol Pharm 2013;70:1035-43.
38. Biswas M, Haldar PK, Ghosh AK. Antioxidant and free-radical-scavenging effects of fruits of Dregea volubilis. J Nat Sci Biol Med 2010;1:29–34.
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How to Cite
Mohana, M., and P. Padma. “FREE RADICAL SCAVENGING ACTIVITY OF THE BACOSIDE FRACTION FROM BACOPA MONNIERI”. International Journal of Current Pharmaceutical Research, Vol. 8, no. 2, Apr. 2016, pp. 61-64,
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