• Anjali Bisht Department of Biotechnology, Govind Ballabh Pant Engineering College, Pauri Garhwal, Uttarakhand, India 246194
  • Manisha Thapliyal Department of Biotechnology, Govind Ballabh Pant Engineering College, Pauri Garhwal, Uttarakhand, India 246194
  • Ajeet Singh Department of Biotechnology, Govind Ballabh Pant Engineering College, Pauri Garhwal, Uttarakhand, India 246194


Objective: To screen and isolate the antibacterial proteins/peptides from seeds of millets.

Methods: Present study was designed to investigate antibacterial activity of proteins/peptides extracted from seeds of different varieties of millet (Finger Millet (PRM I, PRM II), Barnyard millet and Porso millet). The extracted proteins/peptides were checked for antibacterial activity against Bacillus subtilis (MTCC 441), Pseudomonas aeruginosa (MTCC 424), Escherichia coli (MTCC 733) and Salmonella entrica (MTCC 739) bacterial pathogens.

Results: Results indicated that finger millet variety (PRM I) have showed the highest antibacterial activity against P. aeruginosa and S. entrica with a zone of inhibition 22.6±1.18 and 22.3±0.98 respectively. The seed extract of Porso millet has shown the least activity with the gram-positive bacteria with a zone of inhibition 15.6±0.27 mm. The finger millet variety (PRM I) with a molecular weight of 35kDa showed the highest antibacterial activity against Pseudomonas aeruginosa with a zone of inhibition 19 mm.

Conclusion: Millet, a widely growing cereal crop in semi-arid regions, has been known for its medicinal importance to cure fungal and bacterial infections. Results obtained in the present study suggest that antibacterial peptides may be used as potential alternatives for the development of novel antibacterial agents.

Keywords: Millets, Antimicrobial peptides (Amps), Antibacterial Proteins/peptides, Antibacterial activity, SDS-Page


1. Montesinos E. Antimicrobial peptides and plant disease control. FEMS Microbiol Lett 2007;270:1–11.
2. Terras FRG, Schoofs HME, De Bolle MFG, Van FL, Rees SB, Vanderleyden J, et al. Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds. J Biol Chem 1992b;267:15301-9.
3. Kamala G, Vutukuru SS, Rani JU, Meghanath P, Pasha C. Screening of small peptides from various germinating seeds having antimicrobial activity. J Pharm Biol Sci 2016;11:2278-3008.
4. Roberts WK, Selitrennikoff CP. Isolation and partial characterization of two antifungal proteins from barley. Biochim Biophys Acta 1986;880:161-70.
5. Huynh QH, Hironaka CM, Levine EB, Smith CE, Borgmeyer JR, Shah DM. Antifungal proteins from plants. Purification, molecular cloning and antifungal properties of chitinases from maize seeds. J Biol Chem 1992;267:6635-40.
6. Vigers AJ, Roberts WK, Selitrennikoff CP. A new family of plant antifungal proteins. Mol Plant Microbe Interact 1991;4:315-23.
7. Terras FRG, Torrekens S, Leuven FV, Osborn RW, Vartderleyden J, Cammue BPA, et al. A new family of basic cysteine-rich antifungal proteins from Brassicaceae species. FEBS Lett 1993;316:233-40.
8. Terras FRG, Eggermont K, Kovaleva V, Raikhel NV, Osborn RW, Kester A, et al. Small cysteine-rich antifungal proteins from radish: their role in host defense. Plant Cell 1995;7:573-88.
9. Manners DJ, Marshall JJ. Some properties of a α-1,3-glucanase from rye. Phytochemistry (Oxf). 1973;12:547-53.
10. Leah R, Tommerup H, Svendsen I, Mundy J. Biochemical and molecular characterization of three barley seed proteins with antifungal properties. J Biochem 1991;266:1564-73.
11. Fernandez de Caleya R, Gonzalez-Pascual B, Garcia-Olmedo F, Carbonero P. Susceptibility of phytopathogenic bacteria to wheat puro-thionins in vitro. Appl Micro 1972;23:998-1000.
12. Terras FRG, Goderis IJ, Leuven FV, Vanderleyden J, Camimue BPA, Broekaert WF. In vitro antifungal activity of a radish (Raphanus sativus L.) seed protein homologous to nonspecific lipid transfer proteins. Plant Physiol 1992a;100:1055-8.
13. Singh N, Meenu G, Sekhar A, Abraham J. Evaluation of antimicrobial and anticancer properties of finger millet (Eleusine coracana) and pearl millet (Pennisetum glaucum) extracts. Pharma Innovation J 2015;3:82-6.
14. Shahidi F, Chandrasekara A. Millet grain phenolics and their role in disease risk reduction and health promotion: a review. J Funct Foods 2013;5:570–81.
15. Mateen A, Tanveer Z, Janardhan K, Gupta VC. Screening and purification of antimicrobial proteins/peptides from some of the medicinal plants seeds. Int J Pharm Biol Sci 2015;6:774–8.
16. Sripriya G, Chandrasekaran K, Murty VS, Chandra TS. ESR spectroscopic studies on free radical quenching action of finger millet (Eleusine coracana L. Gaertn). Food Chem 1996;57:537–40.
17. Uchoa AF, Souza PAS, Zarate RML, Filho GE, Campos FAP. Isolation and characterization of a reserve protein from the seeds of Opuntia ficus-indica (Cactaceae). Braz J Med Biol Res 1998;31:757-61.
18. Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54.
19. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680-5.
20. Reeves DS. Antibiotic assays. In: Hawkey PM, Lewis DA. Eds. Medical Bacteriology, A Practical Approach. IRL Press: Oxford; 1989. p. 195–221.
21. Malaguti M, Dinelli G, Leoncini E, Bregola V, Bosi S, Cicero AFG, Hrelia S. Bioactive peptides in cereals and legumes: agronomical, biochemical and clinical aspects: a review. Int J Mol Sci 2014;15:21120-35.
22. Mahmood A, Raja GK, Mahmood T, Gulfraz M, Khanum A. Isolation and characterization of antimicrobial activity conferring component(s) from seeds of bitter gourd (Momordica charantia). J Med Plants Res 2012;6:566-73.
23. Parkash A, Ng TB, Tso WW. Purification and characterization of charantin, a napin like ribosome-inactivating peptide from bitter gourd (M. charantia) seeds. J Pept Res 2002;59:197-202.
24. Sheng Q, Yao H, Xu H, Ling X, He T. Isolation of plant insulin from Momordica charantia seeds by gel filtration and RP-HPLC, Braz. J Med Biol Res 2004;27:414-6.
670 Views | 2332 Downloads
How to Cite
Bisht, A., M. Thapliyal, and A. Singh. “SCREENING AND ISOLATION OF ANTIBACTERIAL PROTEINS/PEPTIDES FROM SEEDS OF MILLETS”. International Journal of Current Pharmaceutical Research, Vol. 8, no. 3, July 2016, pp. 96-99,
Original Article(s)