SCREENING AND ISOLATION OF ANTIBACTERIAL PROTEINS/PEPTIDES FROM SEEDS OF MILLETS
Objective: To screen and isolate the antibacterial proteins/peptides from seeds of millets.
Methods: Present study was designed to investigate antibacterial activity of proteins/peptides extracted from seeds of different varieties of millet (Finger Millet (PRM I, PRM II), Barnyard millet and Porso millet). The extracted proteins/peptides were checked for antibacterial activity against Bacillus subtilis (MTCC 441), Pseudomonas aeruginosa (MTCC 424), Escherichia coli (MTCC 733) and Salmonella entrica (MTCC 739) bacterial pathogens.
Results: Results indicated that finger millet variety (PRM I) have showed the highest antibacterial activity against P. aeruginosa and S. entrica with a zone of inhibition 22.6Â±1.18 and 22.3Â±0.98 respectively. The seed extract of Porso millet has shown the least activity with the gram-positive bacteria with a zone of inhibition 15.6Â±0.27 mm. The finger millet variety (PRM I) with a molecular weight of 35kDa showed the highest antibacterial activity against Pseudomonas aeruginosa with a zone of inhibition 19 mm.
Conclusion: Millet, a widely growing cereal crop in semi-arid regions, has been known for its medicinal importance to cure fungal and bacterial infections. Results obtained in the present study suggest that antibacterial peptides may be used as potential alternatives for the development of novel antibacterial agents.Keywords: Millets, Antimicrobial peptides (Amps), Antibacterial Proteins/peptides, Antibacterial activity, SDS-Page
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