ISOLATION OF ANTIBACTERIAL PROTEIN/PEPTIDE FROM FICUS GLOMERATA LEAF


Manisha Thapliyal, Anjali Bisht, Ajeet Singh

Abstract


Objective: To isolate the antibacterial proteins/peptides from Ficus glomerata leaf.

Methods: Present study was designed to investigate antibacterial activity of proteins/peptides isolated from Ficus glomerata leaf. The isolated proteins/peptides were further checked for antibacterial activity against, Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli and Salmonella entrica bacterial pathogens.

Results: The results indicates that a 35kDa of protein were identified and exhibit good antibacterial activity against bacterial pathogen among all strains, Salmonella entrica and Pseudomonas aeruginosa exhibit good results with a clear zone of inhibition.

Conclusion: Ficus glomerata is popular for its medicinal properties against therapeutic potential. In the present study a novel protein with broad spectrum antibacterial activity. Microbes cause severe damage to plants which results in a large economic loss so; this protein can be use as an active agent in agriculture for plant protection and also in the development of novel therapeutic agents.

Keywords


Ficus glomerata, SDS-PAGE, Antimicrobial protein (AMPs), Antibacterial protein/peptide

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About this article

Title

ISOLATION OF ANTIBACTERIAL PROTEIN/PEPTIDE FROM FICUS GLOMERATA LEAF

Keywords

Ficus glomerata, SDS-PAGE, Antimicrobial protein (AMPs), Antibacterial protein/peptide

DOI

10.22159/ijcpr.2016v8i4.15271

Date

18-10-2016

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 8, Issue 4, 2016 Page: 24-27

Online ISSN

0975-7066

Statistics

109 Views | 265 Downloads

Authors & Affiliations

Manisha Thapliyal
Department of Biotechnology, G. B Pant Engineering College, Pauri Garhwal

Anjali Bisht
Department of Biotechnology, G. B Pant Engineering College, Pauri Garhwal

Ajeet Singh
Department of Biotechnology, G. B Pant Engineering College, Pauri Garhwal


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