ANTI-INFLAMMATORY PROPERTIES OF METHANOLIC BARK EXTRACTS OF TERMINALIA BROWNII IN WISTAR ALBINO RATS
Objective: This study investigated the anti-inflammatory properties of methanolic bark extract of Terminalia brownii in Wistar albino rats (Rattus novegicus).
Methods: The experimental animals were divided into six groups of five rats each; normal control, negative control, positive control and three experimental groups. Carrageenan was used to induce inflammation. Diclofenac was used as the reference drug, and the three experimental groups were treated with the extract at the dose levels of 50, 100 and 150 mg/kg bw. The extract was screened for the presence or absence of selected phytochemical secondary metabolites using standard procedures.
Results: The methanolic bark extracts of T. brownii significantly [p Ë‚ 0.05] reduced the carrageenan-induced paw edema by between 1.57%-20.41% while diclofenac reduced it by between 11.12%-25.33%. Phytochemical screening of the extract indicated the presence of alkaloids, flavonoids, phenols, saponins, steroids and terpenoids.
Conclusion: The present study revealed that T. brownii bark extract is a potential candidate for the development of a novel anti-inflammatory formulation.Keywords: Terminalia brownii, Methanolic bark extracts, Anti-inflammatory activity
2. Kamboj VP. Herbal medicine. Curr Sci 2000;78:35-9.
3. Kigen GK, Ronoh HK, Kipkore WK, Rotich JK. Current trends of traditional herbal medicine practice in Kenya: a review. Afr J Pharmacol Ther 2013;2:32-7.
4. Stankov SV. Definition of inflammation causes of inflammation and possible anti-inflammatory strategies. Open Inflammation J 2012;5:1-9.
5. Punchard NA, Whelan CJ, Adcock I. The journal of inflammation. J Inflammation 2004;1:1.
6. Maina GS, Kelvin JK, Maina MB, Muriithi NJ, Kiambi MJ, Umar A, et al. Antinociceptive properties of dichloromethane: methanolic leaf and root bark extracts of Carissa edulis in rats. J Phytopharmacol 2015;4:106-12.
7. Warden SJ. Prophylactic use of NSAIDs by athletes: a risk/benefit assessment. Phys Sports Med 2010;38:132-8.
8. Shukla S, Mehta A. In vivo anti-inflammatory, analgesic and antipyretic activities of a medicinal plant, Caesalpinia bonducella F. Pakistan J Pharm Sci 2015;28(4, Suppl):1517-21.
9. Amaral MIG, Silva MR, Aquino-Neto PF, Teixeira-Neto BA, Moura CT, Melo FL, et al. Biological and Pharmaceutical Bulletin; 2007;30:12-7.
10. Modi C, Mody S, Patel G, Dudhatra G, Avinash K, Madhari A. Toxicopathological overview of analgestic and anti-inflammatory drugs in animals. J Appl Pham Sci 2012;2:149-57.
11. Mwangi BM, Gitahi SM, Njagi JM, Mworia JK, Aliyu U, Njoroge WA, et al. Anti-inflammatory properties of dichloromethane: methanolic leaf extracts of Caesalpinia volkensii and Maytenus obscura in animal models. Int J Curr Pharm Res 2015;7:83-7.
12. Bordgers R, Nascimento MVM, de Carvalho AAV, Valadares MC, de Paula JR, Costa E, et al. Antinociceptive and anti-inflammatory activities of the ethanolic extract from synadenium umbellatum pax. [Euphorbiaceae] leaves and its fractions. J Evidence-Based Complementary Altern Med 2013. Doi.org/10.1155/2013/715650. [Article in Press]
13. Mbwambo ZH, Moshi MJ, Masimba PJ, Kapingu MC, Nondo RS. Antimicrobial activity and brine shrimp toxicity of extracts of Terminalia brownii roots and stem. BMC Complementary Altern Med 2007;7:1.
14. Machumi F, Midiwo JO, Jacob MR, Khan SI, Tekwani BL, Zhang J, et al. Phytochemical, antimicrobial and antiplasmodial investigations of Terminalia brownii. Nat Prod Commun 2013;8:761-4.
15. Khan H, Saeed M, Gilani AH, Muhammad N, Haq IU, Ashraf N. Antipyretic and anticonvulsant activity of Polygonatum verticillatum: comparison of rhizomes and aerial parts. Phytother Res 2013;27:468-71.
16. Vogel HG. Drug discovery and evaluation: pharmacological assays. New York: Springer science and Business Media; 2002.
17. Mwonjoria JK, Kariuki HN, Waweru FN. The antinociceptive and antipyretic effects of Solanum incanum [Linneaus] in animal models. Int J Phytopharmacol 2011;2:22-6.
18. Winter CA, Risley EA, Nuss GW. Carrageenan-induced edema in hind paw of the rat as an assay for anti-inflammatory drugs. Exp Biol Med 1962;111:544â€“7.
19. Bamgbose SOA, Noamesi BK. Studies on cryptolepine inhibition of carrageenan-induced oedema. J Planta Med 198;42:392-6.
20. Harbone JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. London, UK: Chapman and Hal Publishers; 1998.
21. Kotake CK. Practical Pharmacognosy. 4th ed. New Delhi: Vallabh Prakashan publishers; 1994.
22. Chatterjee A, Sen B, Das S, Chatterjee TK. Anti-inflammatory and analgesic activity of methanolic extract of medicinal plant Rhodiola rosea rhizomes. Int J Pharmacol Res Rev 2015;4:1-8.
23. Raghavendra V, Tanga FY, DeLeo JA. Complete Freund's adjuvant-induced peripheral inflammation evokes glial activation and proinflammatory cytokine expression in the CNS. Eur J Neurosci 2004;20:467-73.
24. Van Wauwe JP, Goossens JG. Arabinogalactan-and dextran-induced ear inflammation in mice: differential inhibition by H1-antihistamines, 5-HT-serotonin antagonists, and lipoxygenase blockers. Agents Actions 1989;28:78-82.
25. Ibironke G, Ajiboye K. Studies on the anti-inflammatory and analgesic properties of Chenopodium Ambrosiodes Leaf extracts in rats. Int J Pharmacol 2007;3:111-5.
26. Necas J, Bartosikova L. Carrageenan: a review. Vet Med 2013;58:187-205.
27. Erdemoglu N, Turan NN, Akkol EK, Sener B, Abacioglu N. Estimation of anti-inflammatory, antinociceptive and antioxidant activities on Arctium minus [Hill] Bernh. ssp. minus. J Ethnopharmacol 2009;121:318-23.
28. Thomazzi SM, Silva CB, Silveira DCR, Vasconcellos CLC, Lira AF, Cambui EVF. Antinociceptive and anti-inflammatory activities of Bowdichia virgilioides [sucupira]. J Ethnopharmacol 2010; 127:451-6.
29. Kapewangolo P, Omolo JJ, Bruwer R, Fonteh P, Meyer D. Antioxidant and anti-inflammatory activity of Ocimum labiatum extract and isolated labdane diterpenoid. J Inflammation 2015;12:4.
30. Nivsarkar M, Mukherjee M, Patel M, Padh H Bapu. C. Launaea nudicaulis leaf juice exhibits anti-inflammatory action in acute and chronic inflammation models in rats. Indian Drugs 2009;39:290.
31. Mradu G, Dailya B, Arup M. Studies of anti-inflammatory, antipyretic and analgesic effects of aqueous extract of the traditional herbal drug of rodents. Int Res J Pharm 2013;4:113-20.
32. Arul V, Miyazaki S, Dhananjayan R. Studies on the anti-inflammatory, antipyretic and analgesic properties of the leaves of Aegle marmelos Corr. J Ethnopharmacol 2005;96:159-63.
33. Matthew S, Jain AK, James M, Matthew C, Bhowmik D. Analgesic and anti-inflammatory activity of Kalanchoe pinnata [lam.] pers. J Med Plants Studies 2013;1:24-8.
34. Mitchell JA, Akarasereenont P, Thiemermann C, Flower RJ, Vane JR. Selectivity of nonsteroidal anti-inflammatory drugs as inhibitors of constitutive and inducible cyclooxygenase. Proc Natl Acad Sci 1993;90:11693-7.
35. Safari Z, Kamau JK, Nthiga PM, Ngugi MP, Orinda G, Njagi EM. Antipyretic, anti-inflammatory and antinociceptive activities of aqueous bark extract of Acacia Nilotica [L.] delile in albino mice. J Pain Management Med 2016;2:113.
36. Safari Z, Ngugi MP, Orinda G, Njagi EM. Anti-pyretic, anti-inflammatory and analgesic activities of aqueous stem extract of Cynanchum Viminale [L.] in albino mice. Open Access J Med Aromat Plants 2016;5:236.
37. Falodun A, Okunrobo LO, Uzoamaka N. Phytochemical screening and anti-inflammatory evaluation of methanolic and aqueous extracts of Euphobia heterophylla Linn [Euphorbiaceae]. Afr J Biotechnol 2006;5:529.
38. Hossain E, Mandal SC, Gupta JK. Phytochemical screening and in vivo antipyretic activity of the methanol leaf extract of Bombax malabaricum [Bombacaceae]. Trop J Pharm Res 2006;10:55-60.
39. Tapas AR, Sakarkar DM, Kakde RB. Flavonoids as nutraceuticals: a review. Trop J Pharm Res 2008;7:1089-99.
40. Mencarelli A, Renga B, Palladino G, Distrutti E, Fiorucci S. The plant sterol attenuates inflammation and immune dysfunction in murine models of inflammatory bowel disease. Biochem Pharmacol 2009;78:1214-23.
41. Salminen A, Lehtonen M, Suuronen T, Kaarniranta K, Huuskonen J. Terpenoids: natural inhibitors of NF-ÎºB signaling with anti-inflammatory and anticancer potential. Cell Mol Life Sci 2008;65:2979-99.