ALLAMANDA CATHARTICA LINN.: EXTRACTION AND PHARMACEUTICAL EVALUATION OF VARIOUS EXTRACTS OF LEAVES AND FLOWERS


Meenakshi Fartyal

Abstract


Objective: Extraction and evaluation of the antifungal activity of extracts from leaves and flowers of Allamanda cathartica (Apocynaceae) Linn.

Methods: Leaves and flowers of A. cathartica were collected, dried and extracted by using well-established methods for alkaloids, flavonoids, steroids and crude extracts in polar and non-polar solvents. Extracts were screened for antifungal activity using ‘Disc Diffusion Assay’ against Candida albicans (Yeast), Aspergillus flavus and Tricophyton mentagrophyte (fungi). Inhibition zone (IZ), Activity index (AI), Minimum inhibitory concentration (MIC), Minimum fungicidal concentration (MFC) and Total activity (TA) were studied. Mean and standard deviation has also been calculated.

Results: C. albicans found to be the most susceptible organism while T. mentagrophyte found to be resistant. Alkaloid of leaf (IZ= 22 mm, AI= 0.65±0.02, MIC= 0.156 mg/ml, MFC= 0.078 mg/ml, TA= 278.85 ml/g) showed the best activity against C. albicans. Pet ether extract (IZ= 12 mm, AI= 0.40±0.02, MIC= 0.312 mg/ml, MFC= 0.156 mg/ml, TA= 57.24 ml/g) and water extract (IZ= 13 mm, AI= 0.43±0.02, MIC= 0.312 mg/ml, MFC= 0.156 mg/ml, TA= 49.68 ml/g) of flower also showed very good activities against C. albicans. The range of MIC and MFC found to be 1.25-0.156 mg/ml and 0.625-0.078 mg/ml, respectively.

Conclusion: Results indicate the good antifungal potency of extracts against tested microorganisms. Hence, may be explored for the formation of new antifungal drugs.

Keywords


Antifungal activity, Alkaloid, Flavonoid, Steroid, Polar and non-polar solvents, Disc diffusion assay, Minimum inhibitory concentration, Minimum fungicidal concentration, Total activity

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About this article

Title

ALLAMANDA CATHARTICA LINN.: EXTRACTION AND PHARMACEUTICAL EVALUATION OF VARIOUS EXTRACTS OF LEAVES AND FLOWERS

Keywords

Antifungal activity, Alkaloid, Flavonoid, Steroid, Polar and non-polar solvents, Disc diffusion assay, Minimum inhibitory concentration, Minimum fungicidal concentration, Total activity

DOI

10.22159/ijcpr.2016v8i4.15272

Date

18-10-2016

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 8, Issue 4, 2016 Page: 28-32

Online ISSN

0975-7066

Statistics

39 Views | 82 Downloads

Authors & Affiliations

Meenakshi Fartyal
Laboratory of Plant Tissue Culture and Secondary Metabolites, Department of Botany, University of Rajasthan, Jaipur, India
India


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