NERIUM OLEANDER LINN. IN VITRO ALPHA AMYLASE INHIBITORY POTENTIAL OF STEM AND ROOT EXTRACTS


Meenakshi Fartyal

Abstract


Objective: Extraction and evaluation of the antidiabetic activity of extracts from stem and roots of Nerium oleander (Apocynaceae) Linn.

Methods: Stem and roots of N. oleander were collected, dried and extracted by using well-established methods for alkaloids, flavonoids, steroids and crude extracts in polar and non-polar solvents. Evaluation of their antidiabetic activity was done with salivary alpha-amylase and starch as a substrate using chromogenic DNSA (2,4-Di nitro Salicylic Acid) method and Starch-iodine method. All experiments were performed in 3 different sets each in triplicates. The data are expressed as mean±SEM (standard error of the mean).

Results: The highest inhibition for stem was found in its free flavonoid extract at the concentration of 1.5 mg/ml, with percent inhibition 48.35±1.36 % and an IC50 value of 1.774 g/ml while in case of root, highest inhibition was obtained at 1.5 mg/ml of pet ether extract, with % inhibition 52±0.40 % and IC50 value 1.583 g/ml and at 1.5 mg/ml of methanol extract, with % inhibition 42.12±1.12 % and an IC50 value 1.729 g/ml. 8 (5 of stem and 3 of root) out of 14 tested extracts have shown good inhibitory potential. Extracts of the stem were found to be more potent than root extracts.

Conclusion: Though stem extracts were found to be a more potent hypoglycemic agent than root extracts, however, extracts of both parts have good antidiabetic potential and both might be fruitful in managing the postprandial hyperglycemia.


Keywords


Antidiabetic activity, Alkaloid, Flavonoid, Steroid, Petroleum ether, Methanol, Water, Salivary alpha-amylase, DNSA, Starch

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About this article

Title

NERIUM OLEANDER LINN. IN VITRO ALPHA AMYLASE INHIBITORY POTENTIAL OF STEM AND ROOT EXTRACTS

Keywords

Antidiabetic activity, Alkaloid, Flavonoid, Steroid, Petroleum ether, Methanol, Water, Salivary alpha-amylase, DNSA, Starch

DOI

10.22159/ijcpr.2017v9i2.17378

Date

01-03-2017

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 9, Issue 2, 2017 Page: 37-41

Online ISSN

0975-7066

Statistics

94 Views | 64 Downloads

Authors & Affiliations

Meenakshi Fartyal
Laboratory of Plant Tissue Culture and Secondary Metabolites, Department of Botany, University of Rajasthan, Jaipur, India


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