ASSESSMENT OF EFFECT OF SHODHANA ON PHYTOCHEMICAL AND CHROMATOGRAPHICAL PROFILE OF DIFFERENT LEVELS OF CLASSICAL PROCESSED DANTI (BALIOSPERMUM MONTANUM WILLD.) ROOT
Objective: Ayurveda recommends the use of Danti root after Shodhana (Processing/Purification) where the powder Pippali (Piperlongum Linn.) fruit, honey and Kusha (Desmostachya bippinata Stapf.) leaves are being used. But the additive effect of all these drugs on Danti root are yet to be explored scientifically. Principal component analysis (PCA), a multivariate data analysis technique targeting to assess the discrimination effect of psychic nut, for evaluating the additive effect, can be used to assess the effect of Shodhana on preliminary physicochemical, phytochemical parameters upon four levels of Danti (Baliospermum montanum Willd.) root.
Methods: Roots of raw Danti, after proper botanical authentication, were subjected for classically recommended Shodhana procedure and four groups of Danti root like raw Danti (RD), Classical processed Danti root (CPDR), Kusha processed Danti root (KPDR), water processed Danti root (WPDR) were obtained at various levels of Danti Shodhana. Methanolic macerated extracts of all four Danti root groups were subjected for preliminary physicochemical, phytochemical and chromatographic screening. The obtained data were analyzed with the help of the Un-scrambler Camo Software for multivariate data analysis.
Results: The methanolic and water extractive value of CPDR group is more than remaining sections holding lower ash value and high-intensity colour reaction during phytochemical screenings of steroid, flavonoid etc.
Conclusion: Analysis of PCA technique suggests a similar trend in between RD and KPDR group while CPDR and WPDR on a different in score plot.
2. Charaka Samhita, Ayurveda-Deepika. Commentary of Chakrapanidatta by Kashinatha Shastry and Gorakhanatha Chaturvedi (Ed.), Chaukhamba Surbharati Prakashan, Varanasi, Vimanasthana 01/22; 2007. p. 680.
3. Acharya R. Shodhana: an ayurvedicde toxification technique and its impact on certain medicinal plants. J Ayurveda 2011;5:69-76.
4. Rachana Kamble, Sadhana Sathaye, Shah DP. Evaluation of the antispasmodic activity of different Shodhit guggulu using different shodhan process. Int J Pharm Sci 2008;70:368-72.
5. Mitra S, Shukla VJ, Acharya R. Effect of shodhana (processing) on Kupeelu (Strychnos nux-vomica Linn.) with special reference to strychnine and brucine content. Int Quarterly J Res Ayurveda 2011:1;32:402.
6. Deore SL, Moon KV, Khadabadi SS, Deokate UA, Baviskar BA. Evaluation of toxicity of â€˜Vatsanabhaâ€™ (Aconitum ferox, Ranunculaceae) before and After Shodhana. J Young Pharm 2013;5:3-6.
7. Santosh Kumar Maurya, Ankit Seth, Damiki Laloo, Narendra Kumar Singh, Åšodhana. An ayurvedic process for detoxification and modification of therapeutic activities of poisonous medicinal plants. Anc Sci Life 2015;34:188â€“97.
8. Saxena HO, Brahmam M. The Flora of Orissa. Vol. II. Regional Research Laboratory, Orissa Forest Development Corporation Ltd. Bhubaneswar; 1990. p. 897.
9. Charaka Samhita, Ayurveda-Deepika. Commentary of chakrapanidatta by kashinatha shastry and gorakhanatha chaturvedi. Ed. Chaukhamba Surbharati Prakashan, Varanasi, Kalpasthana12/05; 2007. p. 936.
10. The Ayurvedic Pharmacopeia of India. edition 1st Govt. of India. Ministry of Health and Family welfare, Department of I. S. M. and H., New Delhi, Part-I, Volumeâ€“I; 2004. p. 143.
11. Official Methods of Analysis. 15th Edn. Association of Official Analytical Chemists. Washington DC. USA; 1990.
12. Harbourne JB. Phytochemical methods: a guide to modern techniques of plant analysis. Ghapman and hall, London; 1998. p. 4-120.
13. Kasture AV, Wadodkar SG, Mahadik KR, More HN. Pharmaceutical analysis, Industrial Methods. Vol. II, Nirali Prakashan; 2008.
14. William H. Edi. Official methods of analysis, Association of Official Agricultural chemists Washington. 4th Ed; 1960.
15. Baxi AJ, Shukla VJ, Bhatt UB. Methods of qualitative testing of some Ayurvedic formulations. Jamnagar: Gujarat Ayurved University 2001;5:512.
16. Cook NC, Samman S. Flavonoids-chemistry, metabolism, cardioprotective effects, and dietary sources. J Nutr Biochem 1996;7:66-76.
17. Gemperline P. Editor. Practical Guide to Chemometrics. 2nd edition. Chapter 4. CRS Press. Taylor and Francis group; 2006. p. 342.
18. Glenn J Myatt, Wayne P Johnson. Making sense of data II-A practical guide to data visualization, advanced data mining methods, and applications, John Wiley and Sons, Inc., Hoboken, New Jersey; 2009. p. 126-7.
19. Anonymous. The Ayurvedic Pharmacopoeia of India. Vol. 03. 1st Ed. New Delhi: Govt. of India, Ministry of Health and Family Welfare; 1999. p. 41-2.