PRELIMINARY PHYTOCHEMICAL SCREENING AND EVALUATION OF ANTI-DIARRHOEAL ACTIVITY OF ETHANOLIC EXTRACT OF LEAVES OF CLERODENDRUM INFORTUNATUM
Keywords:Anti-diarrheal, Clerodendrum infortunatum, Castor oil, Loperamide
Objective: To determine preliminary phytochemical Screening and evaluation of Antidiarrheal activity of ethanolic extract of Leaves Clerodendrum infortunatum.
Methods: The ethanolic extract of leaves of Clerodendrum infortunatum was prepared and analyzed for phytochemical constituents using standard method and evaluate scientifically the anti-diarrheal effect using leaves of Clerodendrum infortunatum by castor oil-inducing method. Animals were divided into four groups. Group-I was treated with distilled water 2 ml/kg body weight. Group-II was treated with loperamide 1 mg/kg body weight, Group-III and Group-IV were treated with ethanolic extract of leaves Clerodendrum infortunatum 200 mg/kg and 500 mg/kg body weight respectively.
Results: Various phytochemical analysis revealed the presence of sterols, terpenoids, alkaloids, carbohydrates, tannins, glycoside, saponins, proteins and amino acids. The ethanolic extract of Clerodendrum infortunatum leaves given by oral route to mice at doses of 200 mg/kg and 500 mg/kg significantly showed antidiarrheal activity against castor oil induced diarrhea as compared with control group and standard.
Conclusion: The present study concluded that ethanolic extract of leaves of Clerodendrum infortunatum contain the high presence of phytochemical components and it showed that the leaves of Clerodendrum infortunatum having significant antidiarrheal activity. All doses of the plant extracts showed a significant delay in castor oil-induced diarrhea and this justifies the use of this plant as a herbal remedy against diarrhea.
Pathak K, Das JR. Herbal medicine-a rational approach in health care system. Int J Herbal Med 2013;1:86-9.
Mukherjee PK, Das J, Balasubramanian R, Saha K, Pal M, Saha BP. Anti-diarrhoeal evaluation of Nelumbo nucifera rhizome extract. Indian J Pharmacol 1995;22:262â€“4.
Guerrant RL, Van Gilder T, Steiner TS, Thielman MN, Slutsker L. Practice Guidelines for the management of infectious diarrhoea. J Infect Dis 2001;32:331-51.
Yu LL, Liao JF, Chen CF. Anti-diarrhoeal effect of water extract of evodiae fructus in mice. J Ethnopharmacol 2000;8:39-45.
Abdullahi AL, Agho MO, Amos S, Gamaniel KS, Watanabe C. Antidiarrhoeal activity of the aqueous extract of Terminalia avicennoides roots. Phytother Res 2001;15:431-4.
Valentine NN, Achidi EA, Gonsu HK, Lyonga EE, Mathew DE, Krisztian B, et al. Epidemiology of rotavirus diarrhoea in children under 5 y in Northern Cameroon. Pan Afr Med J 2012;11:73-5.
Longanga OA, Vercruysse A, Foriers A. Contribution to the ethnobotanical, phytochemical and pharmacological studies of the traditionally used medicinal plant in the treatment of dysentery and diarrhoea in Lomela area, Democratic Republic of Congo (DRC). J Ethnopharmacol 2000;5:411-3.
Atta AH, Mouneir SM. Antidiarrhoeal activity of some Egyptian medicinal plant extracts. J Ethnopharmocol 2004;92:303-9.
Sheell R, Kumari N, Kumar J. Preliminary phytochemical screening of methanolic extract of clerodendron infortunatum. IOSR J Appl Chem 2010;7:10-3.
Das S, Bhattacharya S, Biswas M, Kar BN, Kumar S, Haldar PK. Acute and subchronic toxicity study of clerodendrum infortunatum Leaf in adult male albino mice. Am Eur J Sci Res 2011;6:188-91.
Omodamiro OD, Ibeh RC. Evaluation of antidiarrhoeal activities of leaf and fruit of Psidium guajava L. (Myrtaceae) in the experimental animal model. Peak J Med Plant Res 2014;2:58-62.