PRELIMINARY PHYTOCHEMICAL SCREENING AND EVALUATION OF MUSCLE RELAXANT ACTIVITY OF ETHANOLIC EXTRACT OF RHIZOME OF ZINGIBEROFFICINALE


Juri Das, Dipankar Saha

Abstract


Objective: The aim of this study to investigate the Preliminary Phytochemical Screening and evaluation of Muscle relaxant activity of ethanolic extract of Zingiberofficinale (EEZO) belonging to the family Zingiberaceae using Swiss albino mice in comparison with that of standard drug (Lorazepam).

Methods: The phytochemical screening of the EEZO was done as per the standard methods. Then the extract was evaluated for its muscle relaxant activity compared with Control which is Normal saline (0.9% NaCl solution) at a dose of 10 ml/kg and standard drug Lorazepam at a dose of 10 mg/kg p. o by using Rota-rod apparatus. Twenty mice were taken of either sex and are divided into four groups and each group contains five animals. The first group was considered as control, the second group considered as standard and the third and fourth group received extracts (EEZO) at a dose of 100 mg/kg and 200 mg/kg p. o respectively. All the preparations were administered orally.

Results: The Preliminary Phytochemical screening of Ethanolic plant extract of Zingiberofficinale showed the presence of alkaloids, Carbohydrates, phlobotannins, flavonoids, glycosides, saponins, tannin and terpenoids and absence of steroids. The two doses of EEZO i.e. 100 mg/kg (7.8±0.421 sec) and 200 mg/kg (3.07±0.385) significantly reduced the fall of time in the Rota-rod apparatus as compared to control (48.67±1.112) with p value<0.0001. The result is quite satisfying when compared with the standard drug i.e. 10 mg/kg p. o. (6.2±0.331).

Conclusion: The result of the given study demonstrated that the ethanolic extract of Zingiber officinale is having Dose Dependent Muscle Relaxant activity.


Keywords


Zingiberofficinale, Rota-rod apparatus, Lorazepam, Muscle relaxant activity

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References


Jafarikukhdan SH, Hosseini A, Armand R. The application of medicinal plants in traditional and modern medicine: a review of thymus vulgaris. Int J Clin Med 2015;6:635-42.

Ernst E. The efficacy of herbal medicine-an overview. Fund Clin Pharmacol 2005;19:405-9.

Desousa A. Herbal medicines and anxiety disorders: an overview. 2013;1:18-23.

Zingiber officinale information from NPGS/GRIN”, University of Maryland Medical Centre; 2006.

Shakya SR. Medicinal uses of ginger improves growth and enhances immunity in aquaculture. Int J Chem Stud 2015;3:83-6.

Ernst E, Pittler MH. Efficacy of ginger for nausea and vomiting: a systematic review of randomized clinical trials. Br J Anesthesia 2000;84:367-71.

Bailey DN, Briggs JR. An unrecognized drug of abuse. Am J Clin Pathol 2002;117:396-400.

Shrestha BM. Muscle relaxant in 21st century. Kathmandu University Med J 2003;1:60-4.

Wallis TC. Textbook of Pharmacognosy, CBS Publishers and Distributors, New Delhi; 1985. p. 571-6.

Cartmell SM, Gelgor L, Mitchell D. A revised rota-rod procedure for measuring the effect of antinociceptive drugs on motor function in the rat. J Pharmacol Meth 1991;26:149–59.




About this article

Title

PRELIMINARY PHYTOCHEMICAL SCREENING AND EVALUATION OF MUSCLE RELAXANT ACTIVITY OF ETHANOLIC EXTRACT OF RHIZOME OF ZINGIBEROFFICINALE

Keywords

Zingiberofficinale, Rota-rod apparatus, Lorazepam, Muscle relaxant activity

DOI

10.22159/ijcpr.2017v9i5.22129

Date

21-09-2017

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 9, Issue 5 (Aug-Sep), 2017 Page: 13-16

Online ISSN

0975-7066

Statistics

31 Views | 33 Downloads

Authors & Affiliations

Juri Das
Girijananda Chowdhury Institute of Pharmaceutical Science, Azara, Ghy 781017
India

Dipankar Saha
Girijananda Chowdhury Institute of Pharmaceutical Science, Azara, Ghy 781017
India


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