QUALITATIVE AND QUANTITATIVE PHYTOCHEMICALANALYSIS AND DPPH RADICAL SCAVENGING ACTIVITY OF DIFFERENT EXTRACTSOF FLOWER OF BAUHINIA ACUMINATA LINN
Keywords:B acuminata, Photochemicals, Antioxidant, DPPH radical scavenging
Objective: Bauhinia acuminate L (Fabaceae) is a species of flowering shrub native to tropical south eastern Asia. The flowerof B. acuminataare used against various skin diseases,worms, tumours and diabetes. The Indian vaiydas recommended as a remedy to treat severe biliousness.
The qualitative and quantitative estimation of phytochemical constituents in various extracts and the antioxidant potential of flower of Bauhinia acuminata were evaluated.
Methods: All the analysis was done based on standard protocols.
Results: The phytochemical screening reveals the presence of various primary and secondary metabolites like sugar, carbohydrate, amino acid, fat, quinone, steroids, phenol, saponin, alkaloids, and acid content. In quantitative analysis the important secondary metabolites such as alkaloids and phenolic compounds were estimated in all the extracts.
The ethanol and chloroformextract produced significant antioxidant properties in a dose-dependent manner. DPPH free radical scavenging assay of ethanol and chloroform extracts of flower exhibited IC 50 values of 24.44Â±1.201Î¼g/ml and 196.68Â±0.456Î¼g/ml respectively. At 1000 Î¼g/ml concentration both extracts shows maximum radical scavenging activity (98.97% and 85.67 %).Conclusion: The findings of this study indicate that this plant is medicinal with prominent antioxidant property.
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