QUALITATIVE AND QUANTITATIVE PHYTOCHEMICALANALYSIS AND DPPH RADICAL SCAVENGING ACTIVITY OF DIFFERENT EXTRACTSOF FLOWER OF BAUHINIA ACUMINATA LINN
Objective: Bauhinia acuminate L (Fabaceae) is a species of flowering shrub native to tropical south eastern Asia. The flowerof B. acuminataare used against various skin diseases,worms, tumours and diabetes. The Indian vaiydas recommended as a remedy to treat severe biliousness.
The qualitative and quantitative estimation of phytochemical constituents in various extracts and the antioxidant potential of flower of Bauhinia acuminata were evaluated.
Methods: All the analysis was done based on standard protocols.
Results: The phytochemical screening reveals the presence of various primary and secondary metabolites like sugar, carbohydrate, amino acid, fat, quinone, steroids, phenol, saponin, alkaloids, and acid content. In quantitative analysis the important secondary metabolites such as alkaloids and phenolic compounds were estimated in all the extracts.
The ethanol and chloroformextract produced significant antioxidant properties in a dose-dependent manner. DPPH free radical scavenging assay of ethanol and chloroform extracts of flower exhibited IC 50 values of 24.44Â±1.201Î¼g/ml and 196.68Â±0.456Î¼g/ml respectively. At 1000 Î¼g/ml concentration both extracts shows maximum radical scavenging activity (98.97% and 85.67 %).Conclusion: The findings of this study indicate that this plant is medicinal with prominent antioxidant property.
2. Essawi T, Srour M. Screening of some Palestinian medicinal plants forantibacterial activity. J Ethnopharmacol 2000;70:343-9.
3. Kirikar KR, Basu BD. Indian medicinal plants. Vol. 1. Dehar Dun, India: International books Distributers; 1980.
4. Timothy Johnson. CRC ethnobotany desk reference, CRC Press: LLC Boca Taton; 1999. p. 110.
5. Khare CP. Indian medicinal plants: an illustrated dictionary. Springer-Verlag Berlin; 2007. p. 85.
6. Reyadâ€“Ulâ€“Ferdous M, Azam MG, Hossain MD. Phytochemical screening, in vitro membrane stabilizing activities and thrombolytic activities of Lophopetalumjavanicum. Int J Pharm Sci Res 2014;5:350â€“0.
7. Fordin DG. History and concept of big plant genera. Taxon 2004;53:753â€“76.
8. Singh S, Singh SK, Yadav AA. Review on cassia speices; pharmacological, traditional and medicinal aspects in various countries. Am J Phytomed Clin Ther 2013;1:291â€“312.
9. Mohammad FK, Rabeya IS, Ridwan BR, Mohammad AR. Evaluation of cytotoxicity of beta vulgaris and membrane stabilizing activities. Biomed Pharmacol J 2014;17:99-101.
10. Gamble JS. Flora of presidency of Madras. Aldard and son publishing company Ltd; 2004.
11. Taleb-Contini SH, Salvador MJ, Balanco JMF, Albuquerque S, De Oliveria DCR. Antiprotozoal effect of crude extracts and flavonoids isolated from Chromolaena hirsute (Asteraceae). Phyto Res 2004;18:250-4.
12. Patil UH, Gaikwad DK. Phytochemical evaluation and bactericidal potential of Terminaliaarjuna stem bark. Int J Pharm Sci Res 2010;2:614-9.
13. Harbone JB. Phytochemical methods, a guide to modern techniques of plant analysis. India: Springer pvt Hd; 1998.
14. Malick EP, Singh MB. Plant enzymology and Hittoenzymology, Kalyani Publishers, New Delhi; 1980.
15. Fadhil S, Monsef H. Spectrophotometric determination of total alkaloids in Peganumharmala L. using Bromocresol green. Res J Phytochem 2007;1:79-82.
16. Braca A, Sortino C, Politi M. Anti-oxidant activity of flavonoids from Licanialicaniaeflora. J Ethnopharmacol 2002;79:379-81.
17. Bors W, Saran M, Elstner EF. Screening for plant anti-oxidants. In: Linskens HF, Jackson JF. eds. Modern Methods of Plant Analysis-Plant Toxin Analysis-New Series, Berlin: Springer; 1992;13:277-95.
18. Ambasta SP, Ramachandran K, Kashyapa K, Chand R. Useful plants of India. Publication and information directorate. Council of Scientific and Industrial Research, New Delhi; 1986. p. 4337.
19. Michalak A. Phenolic compounds and their antioxidant activity in plant growing under heavy metal stress. Polish J Environ Studies 2006;15:523-30.
20. Rahaman Onike. Phytochemical Screening Tests and Medicinal Values of Plants Active Properties; 2010.
21. Ayitey Smith E, Addae Mensah I. Phytochemical, nutritional and medical properties of some leafy vegetables consumed by Edo people of Nigeria. W Afr J Pharmacol Drug Res 1977;4:7-8.
22. Bonjean K, De Pauw-Gillet MC, Defresne MP, Colson P, Houssier C, Dassonneville L, et al. The DNA intercalating alkaloid cryptolepine interferes with topoisomerase II and inhibits primarily DNA synthesis in B16 melanoma cells. J Ethnopharmocol 1998;69:241-6.
23. Andlauer W, Furst P. Antioxidative power of phytochemicals with special reference to cereals. Cereal Foods World 1998;43:356-9.
24. Jayaprakasha GK, Selvi T, Sakariah KK. Antibacterial and antioxidant activities of grape (Vitisvinifera) seed extract. Food Res Int 2003;36:117-22.