ISOLATION AND IDENTIFICATION OF PHYTOPATHOGENIC FUNGI FROM INFECTED PLANT PARTS


R. Thilagam, G. Kalaivani, N. Hemalatha

Abstract


Objective: The present study deals with the isolation and identification of phytopathogenic fungi. The fungal isolates were Alternaria spp (Tomato early blight), Fusarium oxysporum (Fusarium wilt), Fusarium solani (daming off and root rot), Aspergillus flavus (Ear rot) and Collectotricumspp (Anthracnose).

Methods: They were isolated from infected plant parts and were identified on the basis of colony morphology andlacto phenol cotton blue (LPCB) stains were used to identifymicroscopic examination of spore structures. Pure cultures of the isolates were sub cultured and transferred onto differential media; potato dextrose agar, malt extract agar, czapek yeast extract agar and czapek dox agar for species identification using macro morphological characteristicsThe morphological characteristics of these fungal elements showed various kinds of spores have been identified up to genus/species level.

Results: This study proves rapid and less expensive techniques to validate a primary alarm of contamination.

Conclusion: The fivefungus which were isolated from different plant parts were very effective in distruction of the plant and found that the producionwere reduced due to the infection. This rapid and less expensive techniques to validate a primary alarm of contamination.

Keywords


Phytofungal pathogens, Infected plant part, Microscopic examination, Fusarium, Collectotricum, Alternaria, Aspergillus

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About this article

Title

ISOLATION AND IDENTIFICATION OF PHYTOPATHOGENIC FUNGI FROM INFECTED PLANT PARTS

Keywords

Phytofungal pathogens, Infected plant part, Microscopic examination, Fusarium, Collectotricum, Alternaria, Aspergillus

DOI

10.22159/ijcpr.2018v10i1.24404

Date

15-01-2018

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 10, Issue 1 (Jan-Feb), 2018 Page: 26-28

Online ISSN

0975-7066

Statistics

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Authors & Affiliations

R. Thilagam
Applied Microbiology Laboratory, Department of Microbiology, Periyar University, Salem 636011, Tamilnadu
India

G. Kalaivani
Applied Microbiology Laboratory, Department of Microbiology, Periyar University, Salem 636011, Tamilnadu
India

N. Hemalatha
Applied Microbiology Laboratory, Department of Microbiology, Periyar University, Salem 636011, Tamilnadu
India


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