SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION AMIKACIN IN PURE AND PHARMACEUTICAL DOSAGE FORM


Surya Teja G., Gurupadayya B. M., Venkata Sairam K.

Abstract


Objective: The aim of the study was to develop an easy, sensible and rapid method for the estimation of amikacin in both pure and marketed formulation using the spectrophotometric method.

Methods: Due to lack of chromophoric group in the amikacin, it was derivatized with 0.1 mmol chloranillic acid reagent. For the estimation of amikacin, Shimadzu UV-1700 model spectrophotometer with UV probe software was used. The method was based simple on charge transfer complexation of the drug with a p-chloranilic acid reagent to give a purple coloured product which was measured at 524 nm against blank solution.

Results: The derivatised product of amikacin was detected at a wavelength of 524 nm. Linearity was observed with the concentration range of 20-100 µg/ml with a regression coefficient of 0.9803. Results of all the parameters were within the acceptance criteria with % RSD less than 2.

Conclusion: The spectroscopic method was validated as per ICH guidelines and was found to be applicable for routine quantitative analysis of amikacin in marketed formulations also. The results of linearity, precision, accuracy LOD and LOQ were within the specified limits. The method is highly sensitive, robust, reproducible and specific.

Keywords


Spectrophotometric, Amikacin, Pharmaceutical dosage

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References


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About this article

Title

SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION AMIKACIN IN PURE AND PHARMACEUTICAL DOSAGE FORM

Keywords

Spectrophotometric, Amikacin, Pharmaceutical dosage

DOI

10.22159/ijcpr.2018v10i1.24703

Date

15-01-2018

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 10, Issue 1 (Jan-Feb), 2018 Page: 38-42

Online ISSN

0975-7066

Statistics

13 Views | 2 Downloads

Authors & Affiliations

Surya Teja G.
Department of Pharmaceutical Chemistry, JSS College of Pharmacy, Jagadguru Sri Shivarathreeshwara University, Mysore 570015, Karnataka, India

Gurupadayya B. M.
Department of Pharmaceutical Chemistry, JSS College of Pharmacy, Jagadguru Sri Shivarathreeshwara University, Mysore 570015, Karnataka, India
India

Venkata Sairam K.
Department of Pharmaceutical Chemistry, JSS College of Pharmacy, Jagadguru Sri Shivarathreeshwara University, Mysore 570015, Karnataka, India


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